Analytical method for organic carbon bioactivity involving molecular detection. Provides an analytical method for the real-time quantitative analysis of organic carbon bioactivity against marine bacterial bioactivity. 1) preparing the organic carbon into an aqueous solution to obtain an organic carbon stock solution; 2) filtering and sterilizing the organic carbon stock solution obtained in step 1). 3) Inoculate the bacteria to be analyzed into medium M1 for pre-culture, so that the turbidity of the bacteria reaches OD600=1, centrifuge the bacteria liquid, remove the supernatant, collect the bacteria, add NaCl to the bacteria, and suspend Centrifuge again, suspend the cells with NaCl to obtain the pre-culture solution of the cells; 4) put the stock solution M2 into a culture bottle, then add sterilized distilled water, organic carbon stock solution, and the pre-culture solution of the cells, and prepare the culture bottle Cover it, put it into a micro-activity calorimeter for cultivation, record the heat change in the cultivation process, and analyze the bioactivity of organic carbon. High sensitivity, real-time detection, easy operation, good repeatability and high accuracy.