Product capable of maintaining beauty and keeping young and preparation method of product
A technology for beauty and beauty products, applied in the fields of biotechnology, medical cosmetology and cosmetics, can solve the problems of few cell extract processing steps, microbial pollution, limited beauty and beauty effects, etc., to avoid pollution and deterioration, high biological safety, and significant The effect of skin care beauty effect
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[0027] This embodiment provides an anti-aging product, which includes human stem cell active ingredients, and is obtained by the following method:
[0028] (1) Collection and acquisition of neonatal umbilical cord mesenchymal stem cells: rinse the neonatal umbilical cord tissue with normal saline containing penicillin, cut into small pieces and place them in cell culture dishes for culture;
[0029] (2) The cell culture dish is coated with polylysine, and used after natural drying;
[0030] (3) Use the primary culture medium to perform primary culture on the cells that will adhere to the wall, and replace the cell culture medium every three days; digest the cells, and use the passage medium to stop the digestion after the digestion, according to the ratio of 1:3 for the adherent area pass on;
[0031] (4) Collecting cell culture fluid: collecting the cell culture supernatant before passage to obtain cell culture fluid;
[0032] (5) The collected cell culture fluid is passed ...
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