Method for raising salt-tolerant tomato plants

A cultivation method and tomato technology, applied in the field of agricultural science and technology, can solve the problems of slow recovery of grafted seedlings, underdeveloped roots, weak stress resistance, etc., and achieve the effects of good growth, increased salt tolerance, and enhanced stress resistance.

Inactive Publication Date: 2015-12-02
SHANXIAN COUNTY LYUFENG SEED IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the traditional grafting method is mostly grafting tomato with salt-tolerant tomato rootstock. Because the tomato itself is in the seedling stage and the roots are underdeveloped, the general grafted seedlings with tomato as the rootstock will have weak growth potential and slow seedling recovery speed. Slow, weak resistance and other disadvantages

Method used

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  • Method for raising salt-tolerant tomato plants

Examples

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Effect test

Embodiment 1

[0017] Include the following steps:

[0018] (1) Select the seeds with full grains, soak the seeds in clear water at 5-10°C for 15-60 minutes, put the seeds in 0.5% copper sulfate aqueous solution for 15-20 minutes, take them out and rinse them with sterile water, Under sterile conditions, connect to 1 / 2 MS medium to obtain sterile vaccines;

[0019] (2) When the seedling grows to 6-8cm, the hypocotyl is cut into sections with a length of 0.5-1cm, and inoculated in the callus induction medium, which contains 1mg of gibberellin, benzyl MS medium with 2 mg of adenine, 2 mg of vitamin C, 1 mg of vitamin E, and 2 mg of chitosan;

[0020] (3) Place the callus induction medium in step (2) in a breeding room with a ventilation rate of 20L / min, the temperature in the breeding room is 25-28°C, and the light intensity is 100-200μmol·m -2 ·s -1 , photoperiod: 16h light / 8h dark; subculture once every 20 days, and transfer the subcultured calli twice into the salt stress medium, the sal...

Embodiment 2

[0023] Include the following steps:

[0024] (1) Select the seeds with full grains, soak the seeds in clear water at 5-10°C for 15-60 minutes, put the seeds in 0.5% copper sulfate aqueous solution for 15-20 minutes, take them out and rinse them with sterile water, Under sterile conditions, connect to 1 / 2 MS medium to obtain sterile vaccines;

[0025] (2) When the seedlings grow to 6-8cm, cut their hypocotyls into sections with a length of 0.5-1cm, and inoculate them in callus induction medium, which contains 3 mg of gibberellin, benzyl MS medium with 5 mg of adenine, 5 mg of vitamin C, 3 mg of vitamin E, and 5 mg of chitosan;

[0026] (3) Place the callus induction medium in step (2) in a breeding room with a ventilation rate of 20L / min, the temperature in the breeding room is 25-28°C, and the light intensity is μmol·m -2 ·s -1 , photoperiod: 16h light / 8h dark; subculture once every 20 days, and transfer the subcultured calli twice into the salt stress medium, the salt stre...

Embodiment 3

[0029] Include the following steps:

[0030] (1) Select the seeds with full grains, soak the seeds in clear water at 5-10°C for 15-60 minutes, put the seeds in 0.5% copper sulfate aqueous solution for 15-20 minutes, take them out and rinse them with sterile water, Under sterile conditions, connect to 1 / 2 MS medium to obtain sterile vaccines;

[0031] (2) When the seedling grows to 6-8cm, cut its hypocotyl into sections with a length of 0.5-1cm, and inoculate it in a callus induction medium containing 2 mg of gibberellin, benzyl MS medium with 3 mg of adenine, 4 mg of vitamin C, 2 mg of vitamin E, and 4 mg of chitosan;

[0032] (3) Place the callus induction medium in step (2) in a breeding room with a ventilation rate of 20L / min, the temperature in the breeding room is 25-28°C, and the light intensity is 100-200μmol·m -2 ·s -1 , photoperiod: 16h light / 8h dark; subculture once every 20 days, and transfer the subcultured calli twice into the salt stress medium, the salt stres...

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Abstract

The invention relates to the field of agricultural sciences and technologies and particularly relates to a method for raising salt-tolerant tomato plants. The method for raising the salt-tolerant tomato plants comprises the steps: (1) picking full-seed seeds, cleaning the seeds, then, soaking the cleaned seeds with an aqueous solution of copper sulfate, and carrying out culturing in a sterile environment, so as to obtain sterile seedlings; (2) culturing hypocotyl of the sterile seedlings, so as to obtain calluses; (3) carrying out subculturing once every 20 days, transferring the calluses subjected to twice subculturing into a salt stress culture medium, carrying out subculturing for 3-6 times, then, inoculating survival calluses to a 1 / 2MS culture medium, and carrying out culturing so as to obtain re-grown seedlings; and (4) transplanting the re-grown seedlings to a solid culture medium, and selecting plants growing well as salt-tolerant plants. According to the method, the raised tomato plant variety is high in salt tolerance, rhizomes of the plants are thick and big, the yield of tomatoes is increased, the later-period management is convenient, the cost is reduced, and the economic benefit is increased.

Description

(1) Technical field [0001] The invention relates to the field of agricultural science and technology, in particular to a method for cultivating salt-tolerant tomatoes. (2) Background technology [0002] Secondary salinization of greenhouse soil is a common technical problem in protected cultivation at home and abroad. The threat of salt damage to tomato is becoming more and more serious, resulting in the decline of its yield and quality, which seriously affects the sustainable development of its facility production. Among the methods to improve the salt tolerance of tomato, the most effective method is grafting. However, the traditional grafting method is mostly grafting tomato with salt-tolerant tomato rootstock. Because the tomato itself is in the seedling stage and the roots are underdeveloped, the general grafted seedlings with tomato as the rootstock will have weak growth potential and slow seedling recovery speed. Slow, weak resistance and other shortcomings. (3) C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 任思党任辉刘爱芳
Owner SHANXIAN COUNTY LYUFENG SEED IND CO LTD
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