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Germplasm preservation method for African daisy tissue culture and propagation

A preservation method and germplasm technology, applied in the field of agricultural science, can solve the problems of high cost and large area for germplasm preservation, and achieve the effects of reducing labor force, improving the survival rate of transplanting, and reducing the cost of seed preservation.

Active Publication Date: 2015-12-02
江苏凯信环保材料有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies of conventional gerbera germplasm preservation methods, provide a gerbera germplasm preservation technology with longer preservation period, lower preservation cost and reduced variation, and solve the problem of large area occupied by germplasm preservation, The problem of high cost greatly saves the cost of raw materials, labor and storage space for cultivation, and is easy to operate, stable and reliable, and highly repeatable. It is very suitable for large-scale preservation of gerbera germplasm

Method used

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  • Germplasm preservation method for African daisy tissue culture and propagation
  • Germplasm preservation method for African daisy tissue culture and propagation
  • Germplasm preservation method for African daisy tissue culture and propagation

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Embodiment

[0039] In June 2010, Gerbera germplasm Linglong was selected for germplasm preservation by tissue culture and propagation according to the technical route of the present invention. The detailed steps are as follows:

[0040] 1) Material selection and low temperature pretreatment of explants:

[0041] Select the whole plant of the gerbera variety Linglong that has grown to the budding stage, is robust and disease-free, and has the same germplasm as the parent. First, wash off the root soil and other surface debris with tap water, and then use a brush dipped in detergent to wash it. After one pass, rinse with ultrapure water, immerse the plant roots in 8mg / L KT solution, and pretreat at 2°C for 6 days;

[0042] 2) Vaccination:

[0043] After the low-temperature pretreatment, Linglong was cleaned with ultrapure water and transferred to an ultra-clean workbench, sterilized with 70% alcohol for 15 seconds, rinsed twice with sterile water, and then placed in a 0.1 % mercuric chlor...

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Abstract

The invention discloses a germplasm preservation method for African daisy tissue culture and propagation, which comprises the following technical links: (1) selecting explants and carrying out low-temperature pretreatment; (2) inoculating; (3) inducing adventitious buds and transferring; (4) growing slowly and preserving germplasm; (5) carrying out rejuvenation; and (6) rooting and transplanting. The germplasm preservation method for African daisy tissue culture and propagation, provided by the invention, can preserve African daisy germplasm for 4 years, solves the problems of large floor area, high cost and the like in germplasm preservation, is simple and convenient to operate, stable and reliable in technology and strong in repeatability, and is very suitable for preservation of large-scale African daisy germplasm, thereby being an excellent African daisy germplasm resource preservation method.

Description

technical field [0001] The invention relates to a germplasm preservation method for tissue culture propagation of gerbera, belonging to the field of agricultural science and technology. Background technique [0002] Gerbera ( Gerbera jamesonii Bolus), also known as gerbera and sunflower, is a perennial herb of the Compositae family. It is native to the Devasland region of South Africa, and then introduced to the UK. Now it is widely cultivated all over the world. Gerbera likes warm winters and cool summers. The flowers are large, delicate and upright, elegant and elegant, colorful but not coquettish, as beautiful as clouds, charming and elegant, refreshing and pleasing to the eye, giving people a sense of chic, soft, tranquil and serene. Gerbera is often used to cut flowers and place in vases. If it is paired with green foliage plants such as Kidney Tibetan and Asparagus, it will be even more fascinating. Gerbera is also very suitable for potted plants. It is a good product...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 不公告发明人
Owner 江苏凯信环保材料有限公司
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