Probiotics compound for crowds with blood types of A, B and O and method and application of probiotics compound
An ABO blood type, probiotic technology, applied in the field of biotechnology, to achieve the effect of preventing and curing intestinal diseases
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Embodiment 1
[0042] Example 1: Collection of Human Fecal Samples and Blood Samples
[0043] In order to exclude the influence of individual differences such as host dietary habits, medical history, and age, we recruited 171 male volunteers. All volunteers are 20 to 30 years old and live in Guangdong Province, China. In addition, they had a normal body weight (BMI=18.5-24.9 kg / m2), and those who participated in the survey had not taken antibiotics in the past month. Each participant completed a detailed medical, lifestyle and dietary questionnaire.
[0044] After human fecal samples were collected, they were frozen and stored at -80°C to extract fecal DNA, and the concentration and integrity of the extracted DNA were analyzed by ultramicro spectrophotometer nanodrop2000 and agarose gel electrophoresis. At the same time, medical staff collected 2mL of volunteer blood intravenously into EDTA.2K blood collection tubes, and accurately identified ABO blood types through positive and negative t...
Embodiment 2
[0046] Example 2: 16SrRNA gene sequencing and data processing filtering and OTU statistics:
[0047] Use the qualified samples to construct the library: dilute the qualified genomic DNA as a template, use primer 907R (CCGTCAATTCMTTTGAGTTT) and primer 517F (ACTCCTACGGGAGGCAGCAG) to amplify the V3-V5 region of the 16S rRNA gene, and screen the fragments with magnetic beads; finally, Qualified libraries were used for emPCR and sequencing. Use the data obtained after getting off the machine to carry out the next step of bioinformatics analysis.
[0048] The original data obtained by sequencing has a certain proportion of interference data. In order to make the results of information analysis more accurate and reliable, the original data is first spliced and filtered to obtain valid data. Then, OTUs clustering and species taxonomy analysis were performed based on the valid data, and the OTUs and species annotations were combined to obtain the basic analysis results of OTUs and t...
Embodiment 3
[0050] Example 3: Analysis of differences in intestinal flora of different blood types
[0051] By calculating the Alpha diversity of gut microbes in ABO blood type, we observed a significant decrease in the gut microbial diversity of individuals with blood type A. Table 1 shows that the shannon index is the smallest and the simpson index is the largest in blood type A blood samples, which shows that the species diversity of intestinal bacteria composition of blood type A people is the lowest (Shannon index is 2.59436, P<0.05; Simpson index is 0.16220, P<0.05, t test), B blood type people had the highest diversity of intestinal bacteria (Shannon index was 2.78766, P<0.05; Simpson index was 0.1307, P<0.05, t test). The human intestinal microbial flora is a huge and complex ecosystem, which accounts for about 80% of the total microecological bacteria in the whole body. The diversity of the intestinal flora is very important. There are a large number of diseases such as colorecta...
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