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Application of chlorogenic acid in activation of immune related signaling pathway of organism and research method of chlorogenic acid

An immune-related, signaling pathway technology, applied in the field of biomedicine, can solve the problems of unclear molecular mechanism of action, limiting the accurate grasp and application of biological factors in signaling pathways, etc., to improve reliability, avoid false positive results, and avoid false positives. positive effect

Inactive Publication Date: 2015-12-02
SICHUAN JIUZHANG BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, a large amount of evidence has shown that chlorogenic acid has anti-cancer function. However, the molecular mechanism of chlorogenic acid’s anti-cancer function is still unclear, which limits people’s signals related to its anti-cancer mechanism from the molecular and genetic levels. Accurate grasp and application of pathways, precise targets, and specific biological factors

Method used

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  • Application of chlorogenic acid in activation of immune related signaling pathway of organism and research method of chlorogenic acid
  • Application of chlorogenic acid in activation of immune related signaling pathway of organism and research method of chlorogenic acid
  • Application of chlorogenic acid in activation of immune related signaling pathway of organism and research method of chlorogenic acid

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] 1. Establishment of animal models

[0039] 1.1 Establishment of breast cancer transplanted BALB / C mouse model

[0040] The tumor strain used in this experiment was EMT-6 (mouse breast cancer cells) cells.

[0041]1.2 Recovery, culture and inoculation of EMT-6 cells

[0042] Take the frozen cell line out of the ultra-low temperature freezer at -152°C, thaw in a water bath at 40°C, centrifuge and wash with basal medium, suspend the cells with complete medium, transfer to a cell culture bottle, and place it in a 5% CO2 cell culture incubator Culture at 37°C and change the medium every 2-3 days. After the cells were grown to cover the cell bottle, they were digested with 0.25% trypsin and subcultured to the number of cells required for tumor inoculation.

[0043] After the EMT-6 mouse breast cancer cells have grown to the required number, digest with 0.25% trypsin, blow and centrifuge the culture medium, collect the cells and dilute with PBS, adjust the concentration to ...

Embodiment 2

[0093] Embodiment 2: Prepare freeze-dried powder injection with chlorogenic acid

[0094] 1. Extraction of chlorogenic acid:

[0095] The chlorogenic acid crude drug used in this example is obtained by extracting and purifying Eucommia leaves, with a purity of 99.44%.

[0096] 2. Preparation of chlorogenic acid freeze-dried powder injection

[0097] 2.1 Prescription:

[0098]

[0099] 2.2 Preparation method:

[0100] Add the above prescription into water for injection, stir until completely dissolved, adjust the pH, and use a 0.22μm sterile microporous filter membrane

[0101] After fine filtration, a total of 2000 2ml powder injections were made according to the routine operation of sterile freeze-dried powder injection, each containing 50 mg of chlorogenic acid.

Embodiment 3

[0102] Embodiment 3: prepare pill with chlorogenic acid

[0103] 1. Extraction of chlorogenic acid

[0104] The chlorogenic acid used in this example is extracted and purified from the leaves of Eucommia ulmoides, with a purity of 98.79%.

[0105] 2. Preparation of Chlorogenic Acid Pellets

[0106] 2.1 Prescription

[0107]

[0108]

[0109] 2.2 Preparation method:

[0110] Take an appropriate amount of povidone K30, prepare a solution with absolute ethanol, then take the prescribed amount of chlorogenic acid and starch, mix them uniformly by equal dilution, add the ethanol solution of povidone K30, and stir well to make The soft material was obtained, and 1000 chlorogenic acid pills were prepared by rubbing pills, each pill containing 1 mg of chlorogenic acid.

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Abstract

The invention discloses an application of a chlorogenic acid in activation of an immune related signaling pathway of an organism and a research method of the chlorogenic acid, belongs to the field of biological medicines, and provides a new application of the chlorogenic acid for regulating and controlling the organism immunoreaction from gene and molecular level, namely an application of the chlorogenic acid in activation of the immune related signaling pathway of the organism. A molecular contact between the immune related signaling pathway and the anti-cancer function of the chlorogenic acid is established. A method for accurately regulating and controlling the immune related signaling pathway of the organism through the chlorogenic acid is provided. Through the application, the chlorogenic acid can be further used for guiding treatment of tumors, and can also be used for building related research models for the specific action principle of the immune related signaling pathway or other related applications.

Description

technical field [0001] The invention belongs to the field of biomedicine, and specifically relates to the use of chlorogenic acid in activating immune-related signal pathways of a body and a research method thereof. technical background [0002] Chlorogenic acid (chlorogenic acid CGA), also known as coffee tannic acid, is a phenolic acid composed of caffeic acid (caffeic acid CA) and quinic acid (quinic acid QA), and its chemical name is 3-o-caffeoylquinic acid (3-o- caffeoylquinic acid (CGA). Chlorogenic acid is a phenylpropanoid synthesized by plants through the intermediate product of the pentose phosphate pathway during the process of aerobic respiration. Chlorogenic acid has been widely used in many fields such as food, health care products, cosmetics and pharmaceuticals. Because it widely exists in various common vegetables and fruits, it has a variety of biological activities, such as: cardiovascular protection, anti-oxidation, anti-ultraviolet and anti-radiation ef...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/216A61K48/00A61P37/04A61P35/00
Inventor 张洁朱丽娜
Owner SICHUAN JIUZHANG BIO TECH CO LTD
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