Method for preparing collagen through high-pressure enzymolysis

A collagen and high-pressure enzyme technology, applied in the field of bioengineering, can solve the problems of low collagen production rate, reduced activity, and environmental pollution, and achieve the effects of shortening the enzymatic hydrolysis time, increasing the extraction rate, and avoiding pollution

Inactive Publication Date: 2015-12-02
杨德庆 +1
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  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, the methods for extracting and preparing collagen from animal skin, bone, and scale raw materials mainly include high-temperature cooking + enzymatic hydrolysis, alkali treatment + enzymatic hydrolysis, acid treatment + enzymatic hydrolysis, etc. Alkali treatment not only has high energy consumption, low efficiency, and pollutes the environment, but also changes the characteristics of natural collagen and reduces its activity.
However, if the raw material is directly enzymatically hydrolyzed without pretreatment, the yield of collagen is low and the efficiency is lower, and the raw material is easily spoiled during the extraction process, so it is necessary to apply antiseptic chemicals during the enzymatic hydrolysis process, which will affect Product quality and usage issues

Method used

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  • Method for preparing collagen through high-pressure enzymolysis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] (1) Pretreatment of raw materials: remove foreign matter from pigskin, dry, crush, and pass through a 30-mesh sieve;

[0014] (2) High-pressure enzymatic hydrolysis treatment: Take 10g of pigskin powder, disperse it in the bromelain solution at a ratio of 1:1 by weight of material to liquid (the amount of bromelain added is 7000U / g), add buffer solution to adjust to pH=4 , under the conditions of temperature 70°C and pressure 700Mpa for 0.1h;

[0015] (3) Separation treatment: the enzymatic hydrolysis product was subjected to solid-liquid separation to obtain collagen liquid; the collagen liquid was concentrated and dried to obtain 2.8 g of collagen powder.

Embodiment 2

[0017] (1) Raw material pretreatment: remove foreign matter from the chicken bone, dry it, crush it, and pass it through a 400-mesh sieve;

[0018] (2) High-pressure enzymatic hydrolysis treatment: take 10 g of chicken bone powder, disperse it in the pepsin solution at a ratio of 1:10 by weight of material to liquid (the amount of pepsin added is 1000 U / g), add buffer solution to adjust the pH to 2.2, Enzymolysis for 3.5 hours at a temperature of 30°C and a pressure of 100Mpa;

[0019] (3) Separation treatment: the enzymatic hydrolysis product was subjected to solid-liquid separation to obtain collagen liquid; the collagen liquid was concentrated and dried to obtain 0.9 g of collagen powder.

Embodiment 3

[0021] (1) Raw material pretreatment: remove foreign matter from cattle and sheep bones, dry, crush, and pass through a 40-mesh sieve;

[0022] (2) High-pressure enzymatic hydrolysis treatment: Take 10g of beef and sheep bone powder, disperse it in the papain solution with a weight ratio of 1:7 (the amount of papain added is 4000U / g), add buffer solution to adjust the pH =5, enzymatic hydrolysis for 3.5 hours at a temperature of 50°C and a pressure of 300Mpa;

[0023] (3) Separation treatment: the enzymatic hydrolysis product was subjected to solid-liquid separation to obtain collagen liquid; the collagen liquid was concentrated and dried to obtain 1.4 g of collagen powder.

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Abstract

The invention discloses a method for preparing collagen through high-pressure enzymolysis, and belongs to the technical field of bioengineering. The method comprises the following steps: dispersing smashed animal skin, bone and scale and a feed liquid into an enzyme solution according to the proportion of 1:(1 to 15), adding a buffer solution, adjusting the pH to be 4-9, conducting enzymolysis at the temperature of 30-70 DEG C and the pressure of 100-700 Mpa, and conducting solid-liquid separation on the enzymolysis product so as to obtain a collagen liquid; conducting concentration and drying on the collagen liquid to obtain collagen powder. According to the extracted collagen, the property and the activity of natural collagen are kept to the maximum limit, and compared with the prior art, the collagen extraction rate is obviously improved, and the enzymolysis time is obviously shortened (as low as 0.1 h), so that the efficiency is improved, the energy consumption is reduced, pollution on the environment in the extraction process is also avoided, and the extraction method is efficient, economical and green.

Description

technical field [0001] The invention relates to a method for preparing collagen by using animal skin, bone and scale, in particular to a method for preparing collagen by high-pressure enzymatic hydrolysis, which belongs to the technical field of bioengineering. Background technique [0002] Collagen is the most abundant animal protein in mammals. It mainly exists in animal bones, tendons, muscle sheaths, ligaments, sarcolemma, cartilage and skin. It is an extremely important structural protein in connective tissue and plays a supporting role. The function of organs and protecting the body is also the most important functional protein that composes the intercellular substance. Collagen is also a biomaterial with excellent performance, which is widely used in medical, feed, food, cosmetics and other industrial fields. [0003] At present, the methods for extracting and preparing collagen from animal skin, bone, and scale raw materials mainly include high-temperature cooking +...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 肖建喜杨德庆
Owner 杨德庆
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