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Quick separation method of lotus seed oligosaccharide monomers with prebiotic effect

A separation method and technology of oligosaccharides, applied in the direction of oligosaccharides, chemical instruments and methods, sugar derivatives, etc., to achieve the effects of easy evaporation recovery, avoiding environmental pollution, and increasing separation selectivity

Active Publication Date: 2015-12-16
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As one of the active ingredients of lotus seeds, oligosaccharides are rarely reported at present, and there is a need for a large number of preparations to further study the structure-activity relationship.

Method used

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  • Quick separation method of lotus seed oligosaccharide monomers with prebiotic effect
  • Quick separation method of lotus seed oligosaccharide monomers with prebiotic effect
  • Quick separation method of lotus seed oligosaccharide monomers with prebiotic effect

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Effect test

Embodiment 1

[0045] A method for rapidly separating lotus seed oligosaccharide monomers with a prebiotic effect, which specifically comprises the following steps:

[0046] 1) Material selection and drying: select fresh lotus seeds with full granules and no damage, screen and remove the core, put them in a blast drying oven, and dry them at 40°C until the moisture content is 4%;

[0047] 2) Crushing and sieving: crush the dried lotus seeds in step 1, and pass through a 40-mesh sieve;

[0048] 3) Compounding with water: take the lotus seed powder obtained in step 2), add deionized water and mix well according to the material-to-liquid ratio of 1:5 for compounding;

[0049] 4) Water-washed starch: Pass the compounding solution obtained in step 3) through a 100-mesh sieve, and wash the filter residue with deionized water 4 times the weight of lotus seeds. After combining the filtrate, place it at 4°C for 8 hours, and centrifuge to remove the precipitate. The resulting supernatant is combined ...

Embodiment 2

[0059] A method for rapidly separating lotus seed oligosaccharide monomers with a prebiotic effect, which specifically comprises the following steps:

[0060] 1) Material selection and drying: select fresh lotus seeds with full granules and no damage, screen and remove the core, put them in a blast drying oven, and dry them at 45°C until the moisture content is 5.5%;

[0061] 2) Crushing and sieving: crush the dried lotus seeds in step 1, and pass through a 40-mesh sieve;

[0062] 3) Compounding with water: take the lotus seed powder obtained in step 2), add deionized water and mix well according to the material-to-liquid ratio of 1:5 for compounding;

[0063] 4) Water-washed starch: Pass the compounding solution obtained in step 3) through a 100-mesh sieve, and wash the filter residue with deionized water 5 times the weight of lotus seeds. After combining the filtrate, place it at 4°C for 8 hours, and centrifuge to remove the precipitate. The resulting supernatant is combine...

Embodiment 3

[0073] A method for rapidly separating lotus seed oligosaccharide monomers with a prebiotic effect, which specifically comprises the following steps:

[0074] 1) Material selection and drying: select fresh lotus seeds with full granules and no damage, screen and remove the core, put them in a blast drying oven, and dry them at 50°C until the moisture content is 7%;

[0075] 2) Crushing and sieving: crush the dried lotus seeds in step 1, and pass through a 40-mesh sieve;

[0076]3) Compounding with water: take the lotus seed powder obtained in step 2), add deionized water and mix well according to the material-to-liquid ratio of 1:5 for compounding;

[0077] 4) Water-washed starch: Pass the compounding liquid obtained in step 3) through a 100-mesh sieve, and wash the filter residue with deionized water 6 times the weight of lotus seeds. After combining the filtrate, place it at 4°C for 8 hours, and centrifuge to remove the precipitate. The resulting supernatant is combined wit...

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Abstract

The invention discloses a quick separation method of lotus seed oligosaccharide monomers with prebiotic effect, belonging to the technical field of extraction separation of plant active components. The method comprises the following steps: by using fresh lotus seed as the raw material, carrying out material selection and drying, pulverization and screening, compounding with water, starch water washing, constant-temperature reflux extraction, vacuum filtration, alcohol precipitation, centrifugal concentration, freeze-drying and MPLC (medium pressure liquid chromatography) separation and MPLC secondary separation to obtain the lotus seed oligosaccharide monomer powder with identical or different polymerization degrees, wherein the purity of each lotus seed oligosaccharide monomer can reach 95% or above. Compared with the high-performance liquid chromatography and high-speed counter-current chromatography, the MPLC for separation adopted by the invention has the advantages of high preparation quantity (the sample loading quantity can be milligram level to hectogram level) and short separation time, and the separating column filler can be automatically filled, thereby enhancing the separation selectivity. The method has the advantages of stable technical process and greatly higher separation efficiency, is simple to operate, and can be used for separation preparation of high-purity high-quantity lotus seed oligosaccharide monomers.

Description

technical field [0001] The invention belongs to the technical field of extraction and separation of plant active ingredients, and in particular relates to a rapid separation method of lotus seed oligosaccharide monomers with prebiotic effect. Background technique [0002] With the growth of the economic power of various countries in the world and the continuous improvement of the quality of human life, people's requirements for food functions are also constantly changing, and the demand for health food is also increasing. Among many health foods, oligosaccharides have attracted extensive attention of researchers at home and abroad due to their clear chemical structure and remarkable physiological effects. Functional oligosaccharides are low-level polymerized sugars formed by 2 to 10 monosaccharides linked by specific glycosidic bonds. Excellent physiological activities such as anti-caries and blood lipid lowering have attracted the attention of the world, and its production...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00C07H3/06C07H1/08
Inventor 卢旭郑宝东张怡黄灿灿林姗
Owner FUJIAN AGRI & FORESTRY UNIV
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