Trichoderma viride XJ-3 strain and method for preparing cotton stalk rotten organic fertilizer by using same

A technology of decomposing organic fertilizer and viride trichoderma, which is applied in the field of agricultural microorganism application, green wood enzyme and viride XJ-3 to prepare cotton stalk decomposing organic fertilizer, can solve the problems that have not been reported, increase soil fertility, etc., and achieve strong The effect of growth and reproduction, reducing pollution and increasing fertility

Inactive Publication Date: 2015-12-16
哈密信合棉业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there are many studies and reports on the use of cotton stalks to prepare medium and research on compound fertilizers for improving the soil quality of cotton planting lands, the separation and screening of cotton stalks from the soil of cotton planting sites has strong fermentation ability and can degrade lignin in cotton stalks. Bacteria with cellulose and other components, as well as using Trichoderma viride obtained by self-selection and domestication to ferment cotton stalks to prepare cotton stalk decomposed organic fertilizers are used to improve the soil of cotton planting places, increase soil fertility, and improve cotton seedlings. There is no report on the research that has a certain control effect on anthracnose and anthracnose, and is beneficial to the growth of cotton.

Method used

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  • Trichoderma viride XJ-3 strain and method for preparing cotton stalk rotten organic fertilizer by using same
  • Trichoderma viride XJ-3 strain and method for preparing cotton stalk rotten organic fertilizer by using same
  • Trichoderma viride XJ-3 strain and method for preparing cotton stalk rotten organic fertilizer by using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1: Isolation, screening and identification of Trichodermaviride XJ-3CGMCCNO.10989

[0037] 1. Isolation and screening of strains

[0038] (1) Separation

[0039] The Trichoderma viride used in the present invention is sampled and separated from the soil of the cotton planting field in the Aksu area of ​​southern Xinjiang, and the bacterial strains with certain degrading ability to the cotton stalks are preliminarily screened out according to the analysis of the degradation mechanism of the strains. Use the traditional plate culture method to isolate the microorganisms in the soil layer, purify the strains by the plate streak method, and use different culture temperatures, pH values, and medium as enrichment conditions to screen out a batch of well-growing microbial strains, and select one from them. The strain whose sequence number is XJ-3.

[0040] Separation steps: According to the gradient dilution method, weigh 10g of cotton soil samples in 90mL of sterile...

Embodiment 2

[0050] Example 2: Isolation, screening and identification of Trichodermaviride XJ-3CGMCCNO.10989

[0051] 1. PCR amplification of the rDNAITS segment of Trichoderma viride and its sequencing

[0052] Pick a small amount of single colonies of the XJ-3 strain, put them into an EP tube filled with 25 μL of sterile water, boil at 100°C for 8-10 minutes, and then quickly put them into the ice-water mixture for 5 minutes. Centrifuge at 10000r / min for 5min, store at 4°C, and take the supernatant when used.

[0053] Determination of rDNAITS segment gene sequence and construction of phylogenetic tree: extract the DNA of the bacterial strain according to conventional methods, dilute the universal primer with deionized water, and perform PCR amplification. The primer design is as follows:

[0054] ITS1(F): 5'-TCCGTAGGTGAACCTGCGG-3'

[0055] ITS4(R): 5'-TCCTCCGCTTATTGATATGC-3'

[0056] Determination of gene sequence of rDNAITS segment and construction of phylogenetic tree: Extract the ...

Embodiment 3

[0059] Embodiment three: Utilize Trichodermaviride (Trichodermaviride) XJ-3CGMCCNO.10989 to prepare cotton stalk decomposed organic fertilizer

[0060] Utilize Trichodermaviride (Trichodermaviride) XJ-3CGMCCNO.10989 to prepare the concrete preparation method steps of cotton stalk decomposed organic fertilizer as follows:

[0061] (1) Strain activation culture: prepare the potato dextrose agar medium of Trichodermaviride (Trichodermaviride) XJ-3CGMCCNO.10989 strain, carry out strict aseptic operation after sterilization, transfer from the inclined plane to the plate, and cultivate at 28°C for 5 sky.

[0062] (2) Primary culture: Pick a single colony from the potato dextrose agar solid medium in step (1) and transfer it to a 50mL Erlenmeyer flask containing potato dextrose liquid medium, aseptically operate at 28°C, 120r / min culture 24h-48h.

[0063] (3) Secondary expansion culture: Inoculate the above-mentioned primary culture strain into a 500mL Erlenmeyer flask filled with...

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Abstract

The invention discloses a Trichoderma viride XJ-3 strain and a method for preparing a cotton stalk rotten organic fertilizer by using the same. The method comprises the following steps: carrying out strain separation, screening, breeding and domestication on a cotton land soil sample, carrying out rDNA ITS sequence determination and system development analysis to determine Trichoderma viride XJ-3 CGMCC NO.10989, inoculating into the pulverized cotton stalk (the length is 3-5cm and the water content is 60-65%), adding 30% of urea, 20% of diammonium and a strain protective agent, preparing the strain solution, urea, diammonium and strain protective agent into a fermentation base material, spraying on the pulverized stalk, carrying out fermentation after cotton land tilling and winter irrigation, and standing over the fermentative degradation time of 3 months from autumn to next spring. The method has outstanding technical effects in the aspects of increasing the cotton yield, enhancing the soil fertility and the like.

Description

technical field [0001] The invention relates to the technical field of agricultural microorganism application, in particular, the invention relates to the technical field of preparing cotton stalk decomposed organic fertilizer by a self-selected and screened green wood enzyme and Trichoderma viride XJ-3. Background technique [0002] As an important component of soil and terrestrial carbon pools, soil organic carbon not only contributes to the sustainable development of agriculture, but also affects the capacity, release and turnover of soil carbon pools. Increasing soil organic carbon content, improving crop yield and water and nitrogen use efficiency are important goals for optimal management of farmland in arid regions. Biochar is a kind of organic carbon with high carbon content and strong stability, which can effectively increase soil carbon storage and improve soil fertility and productivity. [0003] my country is an important cotton producing area in the world, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N15/31C05F11/00C05F17/00C12R1/885
CPCY02W30/40
Inventor 朱金武林淑静
Owner 哈密信合棉业股份有限公司
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