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Mycobacterium YC-RL4 and application thereof

A YC-RL4, mycobacteria technology, applied in bacteria, water/sludge/sewage treatment, biochemical equipment and methods, etc., can solve the problems of harsh environmental conditions of industrial wastewater, and achieve good economic value and application prospects. Effect

Active Publication Date: 2015-12-16
中国农业科学院研究生院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the research on the genetic mechanism of its degradation mainly focuses on the cloning and identification of the enzyme genes involved in the degradation from phthalic acid to protocatechol, and has not yet penetrated into the protein research.
Moreover, there is currently a lack of reports on the application in the actual environment, such as the harsh environmental conditions of industrial wastewater, high salt, extreme pH, rich types of pollutants, etc., all of which put forward higher requirements for the tolerance of microorganisms

Method used

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  • Mycobacterium YC-RL4 and application thereof
  • Mycobacterium YC-RL4 and application thereof
  • Mycobacterium YC-RL4 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Isolation and identification of embodiment 1 mycobacterium YC-RL4

[0031] 1. Isolation of strains

[0032] Activated sludge samples were collected from oil-contaminated farmland soil in Dongming County, Heze City, Shandong Province. Under aseptic conditions, 5g of activated sludge samples were inoculated into 20mL of inorganic salt ion medium containing 100mg / LDEHP, and cultured at 30°C and 180rpm. After every 7 days of culture, 1 mL was transferred to fresh inorganic salt medium, and transferred 3 times in a row.

[0033] Streak the acclimatized bacterial solution onto an inorganic salt medium plate containing 100mg / LDEHP, and culture in a 30°C incubator for 3 days. Pick a single colony on the plate and transfer it to inorganic salt culture with a concentration of 100mg / LDEHP for 7 days. Repeat 3 times until a purified strain is isolated, and the strain is named YC-RL4.

[0034] 2. Morphological characteristics of the strain

[0035] The bacterium is a Gram-posit...

Embodiment 2

[0042] Degradation performance test of embodiment 2 mycobacterium YC-RL4

[0043] 1. Mycobacterium YC-RL4 di(2-ethylhexyl) phthalate (DEHP), dicyclohexyl phthalate (DCHP), dimethyl phthalate (DMP), Degradation of di-n-butyl phthalate (DBP) and diethyl phthalate (DEP)

[0044] The degradation effect of mycobacterium YC-RL4 on DEHP, DCHP, DMP, DEP and DBP in inorganic salt medium was detected by gas chromatography (HPLC).

[0045] Inoculate strain YC-RL4 into liquid LB medium for activation, and cultivate to logarithmic growth phase OD 600 =0.8, according to the inoculation amount of 10% by volume, inoculate into the inorganic salt culture medium containing DEHP, DCHP, DMP, DEP and DBP each 100mg / L substrate respectively, as treatment group; , DMP, DEP and DBP each 100mg / L substrate inorganic salt medium was used as the control group, and the control group and the treatment group were respectively set up three times. The control group and the treatment group were cultured at ...

Embodiment 3

[0065] Example 3 Application of Mycobacterium YC-RL4 to the Treatment of Wastewater Containing Various Phthalates

[0066]In the present embodiment, the inorganic salt ion culture medium (not sterilized) containing 50g / LNaCl is used as simulated wastewater (hereinafter referred to as "wastewater"), and a 5.0L fermenter (BioFlo115, New Brunswick Scientific Co., NJ, USA) is utilized to carry out simulated wastewater deal with. Mycobacterium YC-RL4 was cultured in LB liquid medium to logarithmic phase (OD 600 =0.8, the cell concentration is about 1.6×10 8 CFU / mL), adding the prepared bacterial solution to the wastewater to a final concentration of 8 × 10 7 CFU / L, 1.6×10 8 CFU / L, 3.2×10 8 CFU / L, 4.8×10 8 CFU / L, 6.4×10 8 CFU / L and 8.0×10 8 CFU / L wastewater (each treatment is 2L wastewater), and DEHP, DCHP, DMP, DEP and DBP were added to the wastewater at the same time to each concentration of 100mg / L, as a treatment group; at the same time, the same concentration of pollutio...

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Abstract

The invention provides a mycobacterium YC-RL4 capable of degrading various phthalate acid substances. The preserving number of the mycobacterium YC-RL4 is CGMCC No.10993. Within five days, the bacterium can perform 100% degradation on 100 mg / L di-2-ethylhexyl phthalate (DEHP), dicyclohexyl phthalate (DCHP), dimethyl phthalate (DMP), di-n-butyl ortho-phthalate (DBP) and diethyl phthalate (DEP) contained in an inorganic salt culture medium. In addition, the bacterium is wide in range of tolerance to salt ion concentration, pH and temperature. The mycobacterium YC-RL4 can be applied to bio-remediation of phthalate substance environmental pollution, has good application potentials for treating high-salt high-alkali industrial production wastewater of pollutants and has good economical value and application prospects.

Description

technical field [0001] The present invention relates to the fields of microbiology and biodegradation, in particular to mycobacterium YC-RL4 and its application. Background technique [0002] Phthalate Acid Esters (PAEs for short), also known as phthalates, is a general term for esters formed by phthalic acid. Phthalates are mainly used in the preparation of polyvinyl chloride materials and act as plasticizers. As a plasticizer, the content of PAEs in plastic products accounts for about 20% to 30%, or even as high as 50%. Since the advent of polyvinyl chloride (PVC) in the 1950s, PAEs have been produced on a large scale. At present, it occupies more than 80% share of the global plasticizer market. In addition to being plasticizers, PAEs are also widely used as raw materials for the production of paints, adhesives, insect repellants, cosmetics, fragrances and lubricants. In Germany, the annual consumption of PAEs reaches 400,000 tons, and the global annual usage of PAEs i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/32C02F101/34
Inventor 闫艳春任磊贾阳王俊欢
Owner 中国农业科学院研究生院
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