Biological preparation method of red nano selenium

A bio-preparation and nano-selenium technology, which is applied in the field of bio-preparation of red nano-selenium, can solve the problems of high cost and complicated process, and achieve the effect of low manufacturing cost, simple production process and reduced production cost

Active Publication Date: 2018-08-10
YICHUN UNIVERSITY
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Problems solved by technology

When preparing nano-selenium by chemical method, there is an equivalent relationship between the reducing agent and selenium, the process is complicated, and the cost is high
[0006] We found that chlorella can convert inorganic selenium (such as sodium selenite, etc.) into red elemental selenium, and use chlorella to reduce inorganic selenium to prepare nano-selenium, especially red nano-selenium or nano-selenium. to report

Method used

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  • Biological preparation method of red nano selenium
  • Biological preparation method of red nano selenium
  • Biological preparation method of red nano selenium

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Embodiment

[0017] Embodiment: each material described in the embodiment of the present invention can be obtained by commercially available methods;

[0018] Materials and methods used in the present invention:

[0019] The algal species used in the present invention is Chlorella pyrenoidosa purchased from the Institute of Hydrobiology, Chinese Academy of Sciences, numbered FACHB-9;

[0020] Equipment used: High-speed refrigerated centrifuge, pH meter, UV-Vis spectrophotometer, biological clean bench, autoclave, light incubator, and transmission electron microscope are all commercially available laboratory instruments;

[0021] Raw materials used: sodium selenite, sodium molybdate, concentrated sulfuric acid, perchloric acid, concentrated hydrochloric acid, ascorbic acid, hydrogen bromide, phenol, DAB (3,3'-diaminobenzidine), toluene, etc. are all analytically pure;

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Abstract

The invention aims to provide a biological preparation method of red nano selenium, which comprises the following steps: by using Chlorella as a biological material and a seleniferous inorganic salt as a selenium source, inoculating the Chlorella into the seleniferous salt culture medium, culturing under lighting conditions, and controlling the culture temperature at 22-28 DEG C to prepare the red nano selenium. In order to overcome the defects in the prior art, the Chlorella used as the culture material is cultured in the seleniferous salt BG-11 culture medium under certain light and temperature conditions to prepare the red nano selenium, thereby lowering the production cost of the nano selenium and developing new medicinal value and health care functions for pyrenoid Chlorella. The method is environment-friendly in the production process, and can not cause environmental pollution.

Description

Technical field: [0001] The invention relates to a preparation method of nano-selenium, in particular to a biological preparation method of red nano-selenium. technical background: [0002] Selenium is a trace element that has important biological effects on the human body. Selenium exists in a variety of selenoproteins in the form of selenocysteine. Selenium not only participates in the physiological and pathological processes of endemic diseases such as cataract, Keshan disease and Kaschin-Beck disease, but is also closely related to the occurrence of various tumors. [0003] The absorption and utilization efficiency of different selenium sources in the human body varies greatly. Inorganic selenium sources absorb quickly, have a low half-to-dead dose, and have a narrow safety range; organic selenium sources are relatively safe but absorb slowly, and the utilization rate of the body is low; general elemental selenium cannot be absorbed by living organisms. Red nano-seleni...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P3/00C12R1/89
Inventor 卢其能周琨徐长豪李润根
Owner YICHUN UNIVERSITY
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