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Method for preparing fungus hyperbranched polysaccharide-xanthan gum hydrogel bracket

A technology of xanthan gum and polysaccharide, which is applied in the directions of pharmaceutical formulations, inactive medical preparations, pharmaceutical sciences, etc., can solve the problems of easy water solubility, easy damage, poor water resistance and mechanical properties, etc., and achieves low cost. , large amount of embedding, the effect of improving drug efficacy

Inactive Publication Date: 2015-12-23
WUHAN TEXTILE UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the problems of xanthan gum physical hydrogel are: easy to dissolve in water, poor water resistance and mechanical properties, easy to damage
The disadvantage of this method is that the cross-linking agent used is Ca 2+ , relying on the electrostatic force to form a cross-linked network, resulting in a physical hydrogel

Method used

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  • Method for preparing fungus hyperbranched polysaccharide-xanthan gum hydrogel bracket
  • Method for preparing fungus hyperbranched polysaccharide-xanthan gum hydrogel bracket
  • Method for preparing fungus hyperbranched polysaccharide-xanthan gum hydrogel bracket

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preparation example Construction

[0020] A method for preparing fungal hyperbranched polysaccharide-xanthan gum hydrogel scaffold, said preparation method comprising the following steps:

[0021]a. The dried sclerotia of tiger milk mushroom was crushed, and the fat was removed by Soxhlet extraction with ethyl acetate and acetone for 6 hours in sequence. Both ethyl acetate and acetone were chemically pure reagents. Then soak the sclerotium of tiger milk mushroom after fat removal in physiological saline at a temperature of 80°C for 2 hours, and centrifuge; the residue is soaked at a high pressure of 120°C for 30 minutes, wherein every 100g of tiger milk mushroom sclerotia is soaked in 1L of physiological saline, Centrifuge at 8000 rpm for 20 minutes to obtain the extract, cool the extract and then centrifuge and collect the residue; the residue is centrifugally washed with deionized water and freeze-dried to obtain the highly branched polysaccharide of tiger milk mushroom, which can also be dried by other method...

Embodiment 1

[0027] Crush 500g of dried tiger milk mushroom sclerotia, perform Soxhlet extraction with ethyl acetate and acetone for 6 hours to remove fat, then soak the fat-free tiger milk mushroom sclerotia in 5L of normal saline at 80°C for 2 hours, centrifuge , the residue was soaked in 5L of normal saline at a high pressure of 120°C for 30 minutes, centrifuged to obtain an extract, the extract was cooled and then centrifuged to collect the residue, the residue was centrifugally washed with deionized water and freeze-dried to obtain the hyperbranched polysaccharide of tiger milk mushroom. Dissolve 0.5g of hyperbranched polysaccharides from tiger milk mushroom in 25mL of NaOH aqueous solution with pH=12, stir magnetically for 2 hours to prepare hyperbranched polysaccharide solution of tiger milk mushroom with a concentration of 2% w / v, add 0.125g xanthan gum Into the above-mentioned tiger milk mushroom hyperbranched polysaccharide solution with a concentration of 2%w / v, continue magnetic...

Embodiment 2

[0029] Crush 500g of dried tiger milk mushroom sclerotia, perform Soxhlet extraction with ethyl acetate and acetone for 6 hours to remove fat, then soak the fat-free tiger milk mushroom sclerotia in 5L of normal saline at 80°C for 2 hours, centrifuge , the residue was soaked in 5L normal saline at a high pressure of 120°C for 30 minutes, centrifuged to obtain an extract, the extract was cooled and then centrifuged to collect the residue, the residue was centrifugally washed with deionized water and freeze-dried to obtain highly branched tiger milk mushroom polysaccharide. Dissolve 0.5g of hyperbranched polysaccharides from tiger milk mushroom in 25mL of NaOH aqueous solution with pH=13, stir magnetically for 2 hours to prepare hyperbranched polysaccharide solution of tiger milk mushroom with a concentration of 2% w / v, add 0.25g xanthan gum Into the above-mentioned concentration of the tiger milk mushroom hyperbranched polysaccharide solution of 2%w / v, continue magnetic stirring...

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Abstract

The invention discloses a method for preparing a fungus hyperbranched polysaccharide-xanthan gum hydrogel bracket, and belongs to the technical field of natural macromolecular materials. The preparation method comprises the following steps: dissolving pleurotus tuber-regium hyperbranched polysaccharide and xanthan gum into a NaOH aqueous solution according to different mass ratios, generating crosslinking reaction by an obtained pleurotus tuber-regium hyperbranched polysaccharide and xanthan gum solution and a sodium trimetaphosphate aqueous solution under the temperature of 37 DEG C for 10 minutes to 48 hours, and obtaining the fungus hyperbranched polysaccharide-xanthan gum hydrogel bracket. The method disclosed by the invention is easy and convenient to operate; the adopted raw materials are abundant in source; an adopted crosslinking agent is water-soluble and nontoxic; furthermore, the prepared bracket material has medicament controlled release performance, and is high in mechanical property and biocompatibility. The fungus hyperbranched polysaccharide-xanthan gum hydrogel bracket can be used for preparing an artificial tissue bracket and a food or nutritional substance and medicament controlled release carrier.

Description

technical field [0001] The invention relates to a method for preparing fungal hyperbranched polysaccharide-xanthan gum hydrogel scaffold. Belonging to the technical field of natural polymer materials, the fungal highly branched polysaccharide-xanthan gum hydrogel three-dimensional penetrating porous scaffold can be widely used in industries such as tissue engineering materials, biomedical materials, and drug controllable release. Background technique [0002] Polymer hydrogel has a certain degree of elasticity and a three-dimensional through-porous structure, which is suitable for simulating the extracellular matrix, providing a three-dimensional microenvironment suitable for cell growth and biophysical signals between cells and the extracellular matrix, and maintaining the normal phenotype and physiology of cells. Function. Polysaccharides are biological macromolecules that widely exist in nature. They participate in various life activities of cells and produce a variety o...

Claims

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Application Information

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IPC IPC(8): A61L27/52A61L27/56A61L27/54A61L27/20A61L27/12A61K47/36
Inventor 陶咏真张如权徐卫林王哲柏自奎周应山
Owner WUHAN TEXTILE UNIV
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