A method for mixed fermentation of Lactobacillus acidophilus and Acetobacter
A technology of Lactobacillus acidophilus and Acetobacter, which is applied in the field of microbial fermentation, can solve the problems of separate fermentation of Lactobacillus acidophilus and Acetobacter, and achieve the effects of maintaining high activity, high equipment utilization rate, and increasing content
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Embodiment 1
[0030] A method for mixed fermentation of Lactobacillus acidophilus and Acetobacter acetobacter, the specific steps are as follows:
[0031] S1. Lactobacillus seed preparation: Inoculate Lactobacillus acidophilus in MRS basal liquid medium (recipe in Table 1), culture at 36°C for 22 hours, and use as seeds for later use.
[0032] S2. Acetobacter seed preparation: Acetobacter was inoculated in Acetobacter basal liquid medium (recipe is shown in Table 2), cultured on a shaker at 28°C for 22 hours, and used as seeds for later use.
[0033] S3. Mixed fermentation of Lactobacillus and Acetobacter: Add 2.8% glucose, 1.8% soluble starch and 0.4% fructooligosaccharide to the MRS basal medium in Table 1, and inoculate the Lactobacillus seeds in step S1 according to the inoculum size of 4.8%, 36 After anaerobic fermentation at ℃ for 15 hours, 3.3% absolute ethanol was added to the fermentation broth, and the Acetobacter seeds of step S2 were inoculated with an inoculum amount of 8%, ana...
Embodiment 2
[0039] A method for mixed fermentation of Lactobacillus acidophilus and Acetobacter acetobacter, the specific steps are as follows:
[0040] S1. Lactobacillus seed preparation: inoculate Lactobacillus acidophilus in MRS basal liquid medium (recipe in Table 3), culture at 37°C for 23 hours, and use as seeds for later use.
[0041] S2. Acetobacter seed preparation: inoculate Acetobacter in Acetobacter basal liquid medium (recipe in Table 4), culture on a shaker at 29°C for 23 hours, and use as seeds for later use.
[0042] S3. Mixed fermentation of Lactobacillus and Acetobacter: Add 2.9% glucose, 1.9% soluble starch and 0.5% fructooligosaccharide to the MRS basal medium in Table 3, and inoculate the Lactobacillus seeds in step S1 according to the inoculation amount of 4.9%, 37 After anaerobic fermentation at ℃ for 16 hours, 3.4% absolute ethanol was added to the fermentation broth, and the Acetobacter seeds of step S2 were inoculated with an inoculum amount of 9%, anaerobic mixe...
Embodiment 3
[0048] A method for mixed fermentation of Lactobacillus acidophilus and Acetobacter acetobacter, the specific steps are as follows:
[0049] S1. Lactobacillus seed preparation: Lactobacillus acidophilus was inoculated in MRS basal liquid medium (see Table 5 for the formula), cultured at 38°C for 24 hours, and used as seeds for later use.
[0050] S2. Acetobacter seed preparation: Inoculate Acetobacter in Acetobacter basal liquid medium (recipe in Table 6), culture on a shaker at 30°C for 24 hours, and use as seeds for later use.
[0051] S3. Mixed fermentation of Lactobacillus and Acetobacter: Add 3.0% glucose, 2.0% soluble starch and 0.6% fructooligosaccharide to the MRS basal medium in Table 5, and inoculate the Lactobacillus seeds in step S1 at an inoculum size of 5.0%, 38 After anaerobic fermentation at ℃ for 17 hours, 3.5% absolute ethanol was added to the fermentation liquid, and the Acetobacter seeds of step S2 were inoculated with 10% inoculum amount, mixed anaerobic f...
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