Compound traditional Chinese medicine composition and application thereof to resistance to prostate cancer

A prostate cancer and composition technology, applied in the field of medicine, can solve the problems of poor economic affordability of patients, limited treatment effect, high cost, etc., and achieve the effect of inhibiting proliferation, improving sensitivity, and treating advanced castration-resistant prostate cancer

Active Publication Date: 2015-12-30
EAST CHINA NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The latest drugs approved by the U.S. Food and Drug Administration (FDA) for the treatment of CRPC include Abiraterone (Abiraterone) and MDV3100 (Enzalutamide). The former inhibits the synthesis of androgen, and the latter is an antagonist of the androgen receptor. Both of these drugs target the androgen receptor signaling pathway, and have achieved significant curative effects in the treatment of castration-resistant prostate cancer. However, after the above-mentioned endocrine therapy and androgen receptor-targeted drug treatment, the patient still develops drugs. Resistance, the treatment effect is very limited, and the cost is expensive, and the patient's economic ability is poor
In addition, the side effects caused by radiotherapy and chemotherapy are also the bottleneck of clinical treatment.

Method used

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  • Compound traditional Chinese medicine composition and application thereof to resistance to prostate cancer
  • Compound traditional Chinese medicine composition and application thereof to resistance to prostate cancer
  • Compound traditional Chinese medicine composition and application thereof to resistance to prostate cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0104] Embodiment 1: the preparation of Chinese medicine compound composition of the present invention

[0105] The traditional Chinese medicine compound composition with anti-prostate cancer effect in this example is made from the following raw materials: 9 parts of Ailanthus sinensis bark, 30 parts of Hedyotis diffusa, 15 parts of Curcuma zedoary, 30 parts of Salvia miltiorrhiza, 15 parts of Prunella vulgaris, and 9 parts of licorice , adding 15 times the volume of the traditional Chinese medicine compound composition in distilled water and soaking for 3 hours; decocting in an automatic Chinese medicine decoction device, the number of decoctions is 2 times, and decocting for 1 hour each time; Open the cover on the automatic decoction device and evaporate to concentrate until the final volume is 70 ml; the final concentration of the mother liquor of Chinese medicine decoction obtained by concentration is about 1.5 g of crude drug / ml. Filter and sterilize the obtained concentr...

Embodiment 2

[0107] Example 2: Inhibition of the proliferative activity and clone formation of the compound Chinese medicine composition of the present invention on different prostate cancer cells and the induction of apoptosis on different prostate cancer cells

[0108] Technical method:

[0109] 1. Cell Culture

[0110] The cells (LNCaP, 22RV1, PC-3, PNT1A) used in this experiment were all purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences; the other cell line came from primary isolated prostate cancer cells derived from tumor tissue of prostate cancer patients in Shanghai Changhai Hospital ( PCa primary cells), the cells were cultured in a constant temperature incubator at 37 degrees Celsius (humidity 95%, CO 2 concentration 5%), the medium was RPMI-1640 (Gibco) containing 10% fetal bovine serum (Front).

[0111] 2. Determination of cell proliferation by SRB (sulfonyl rhodamine) method

[0112] Different prostate cancer cell lines in 5×10 3 Each / well density wa...

Embodiment 3

[0120] Example 3: Inhibition of the compound Chinese medicine composition of the present invention on the migration and invasion of prostate cancer cells

[0121] Technical method:

[0122] 1. Streak Migration Experiment

[0123] In order to study whether the Chinese medicine compound composition of the present invention inhibits the lateral migration of prostate cancer cells, PC-3 cells are seeded into 6-well plates, and the 2 Routine culture in the incubator for 24 hours until the cells grow to 100% full. Replace the serum-free medium and continue to culture for 12 hours. In the culture wells full of cells, scratch with a 200 microliter sterile pipette tip, wash the cells twice with PBS after scratching, absorb the floating cells, and add 1 ml of complete medium to each well. Add different concentrations of traditional Chinese medicine compound compositions to the cell culture wells, and place the culture plate in CO 2 Incubator, 37 degrees Celsius to continue routine cu...

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Abstract

The invention discloses a compound traditional Chinese medicine composition and application thereof to resistance to prostate cancer. The compound traditional Chinese medicine composition is mainly prepared from the following traditional Chinese medicine raw materials in parts by weight: 4-12 parts of bark of ailanthus altissima, 12-30 parts of spreading hedyotis herb, 3-15 arts of curcuma zedoary, 15-30 parts of salvia miltiorrhiza, 5-15 parts of selfheal and 4-12 parts of liquorice. According to the compound traditional Chinese medicine composition having a prostate cancer-resistant effect, a raw material composition formula is selected according to the traditional Chinese medicine theory and pathogenesis of prostate cancer, all components are scientific and reasonable in ratio, and pharmacological experiment and preclinical experiment results show that the compound traditional Chinese medicine composition provided by the invention has a good prostate cancer-resistant effect, not only can obviously restrain the proliferation and growth of the prostate cancer, but also can restrain migration, invasion and transfer of the prostate cancer, obviously improve the survival quality of tumor-bearing mice, and improve all immune indexes of the tumor-bearing mice, is convenient and rapid in administration route, has no obvious adverse effects, can be developed to be a new-generation prostate cancer-resistant drug and has high potential of becoming a good prostate cancer treatment prescription.

Description

technical field [0001] The invention relates to the technical field of medicine, in particular to a traditional Chinese medicine compound composition and its application in preparing anti-prostate cancer. Background technique [0002] Prostate cancer (PCa), as the most common malignant tumor of the male reproductive system, is a disease that occurs frequently in elderly men, and its incidence increases with age. The incidence of prostate cancer is unevenly distributed around the world. Every year, there are more than 900,000 new cases worldwide, of which more than 260,000 cases die due to the progression of prostate cancer. The incidence of prostate cancer in European and American countries is significantly higher than that in Asia and Africa. In the United States, prostate cancer accounts for the first incidence rate and the second death rate of male tumors in the United States. In my country, the incidence of prostate cancer has increased by 70% compared with the past, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/9066A61P35/00
CPCA61K36/9066
Inventor 易正芳胡美纯刘明耀
Owner EAST CHINA NORMAL UNIV
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