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Rapid screening method for magnesium-rich rice

A screening method and rice technology, applied in the fields of agronomy, rice, and breeding, can solve the problems of not being suitable for large-scale screening of rice breeding, tedious sample pretreatment procedures, and high measurement costs, and achieve an effective single-plant screening method, sensitive color development and The effect of stable and accurate cost

Inactive Publication Date: 2016-01-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current determination methods of mineral content in rice generally require expensive instruments and cumbersome sample pretreatment procedures; not only the determination cost is high, but also the magnesium content of rice cannot be determined quickly and efficiently, so it is not suitable for the rice breeding process. bulk screening

Method used

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  • Rapid screening method for magnesium-rich rice
  • Rapid screening method for magnesium-rich rice
  • Rapid screening method for magnesium-rich rice

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1: Take Shengtai No. 1 mature rice variety and Zhenong 35 mature rice line as the materials to be tested, and Jiayu 293 variety as the control; after the rice to be tested and the standard rice are dehulled, the brown rice of the two is obtained respectively; Get the rice brown rice to be tested and the standard rice brown rice that are basically the same size and carry out the following operations respectively:

[0043] 1. Put 4 grains of brown rice into a 25mL glass test tube, then add 1mL of NaOH solution with a mass concentration of 4%, gelatinize in a constant temperature incubator at 35°C for 6h, take it out and vibrate on an oscillator for 2min, the oscillation frequency is 300~350r / min.

[0044] 2. Add 0.5 mL of 2M hydrochloric acid to the resultant in step 1 for neutralization.

[0045] 3. in the gain of step 2, adding mass concentration is 0.5mL of 20% triethanolamine solution, mass concentration is 0.5mL of polyvinyl alcohol solution 0.5mL, mass conc...

Embodiment 2

[0057] Example 2: Verification test of the color contrast of the reactants between rice varieties (lines) with different magnesium contents and the control Jiayu 293 brown rice.

[0058] Check according to the method described in Example 1, the dyeing comparison results of magnesium content in brown rice (Shengtai No. 1, Zhuyunnuo, Jiayu 293, Zhenong 35, Zhenong 37) of different rice varieties (lines) are as follows: Figure 4 shown. The results showed that the color of Shengtai 1 / Zhuyunnuo, Jiayu 293, Zhenong 37 / Zhenong 35 deepened successively. In the color chart, the color of the reactant of Shengtai No. 1 and the reactant of Zhuyunnuo are light reddish brown, the color of the reactant of Jiayu 293 is reddish brown, the color of the reactant of Zhenong 35 and the reactant of Zhenong 37 are dark red brown.

[0059] The results showed that the color of the brown rice reactant was directly proportional to the magnesium content of the rice flour, and the darker the color, the...

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Abstract

The invention discloses a rapid screening method for magnesium-rich rice. The method includes performing the following steps on to-be-detected brown rice and standard brown rice respectively: conducting brown rice gelatinization; adding hydrochloric acid into the gelatinization product to perform neutralization; then adding a triethanolamine solution, a polyvinyl alcohol solution, a hydroxylamine hydrochloride solution, a Titan yellow staining solution and a sodium hydroxide solution to a constant volume, performing oscillation and shaking the mixture evenly, and then carrying out standing for 8-12min; taking the supernatant so as to obtain the brown rice reactant of the to-be-detected rice and the brown rice reactant of the standard rice; and performing color comparing: when the color of the to-be-detected brown rice reactant is darker than that of the brown rice reactant of the standard rice, the magnesium content of the to-be-detected brown rice is higher than that of standard rice, and the darker the color is, the higher the magnesium content is; otherwise, when the color of the to-be-detected brown rice reactant is lighter than that of the brown rice reactant of the standard rice, the magnesium content of the to-be-detected brown rice is lower than that of standard rice, and the lighter the color is, the lower the magnesium content is.

Description

technical field [0001] The invention relates to a rapid screening method for the magnesium content of brown rice, and mainly relates to the fields of agronomy, breeding and rice. Background technique [0002] Rice is the second largest food crop in the world, and it is also the main food crop in my country. Its nutritional content is directly related to the health of consumers. However, the content of some important mineral elements necessary for the human body in many rice varieties currently promoted in production is obviously insufficient. Magnesium is an essential element to maintain the life activities of the human body, and has the functions of regulating nerve and muscle activity, enhancing endurance and so on. In addition, magnesium is also the "nemesis" of high blood pressure, high cholesterol, and high blood sugar, and helps prevent and treat stroke, coronary heart disease, and diabetes. Magnesium deficiency in the body can cause spasm of the arteries that supply ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/29G01N1/28
Inventor 石春海张世玺金哓丽
Owner ZHEJIANG UNIV
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