Natural protein/polycaprolactone nanofiber electrospun membrane, and preparation and application thereof

A natural protein and polycaprolactone technology, applied in the field of biomedicine, can solve the problem that the scaffold cannot accurately simulate the myocardial matrix environment, and achieve good biomechanical properties, promote cell adhesion, and the effect of easy material

Inactive Publication Date: 2016-01-13
RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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  • Abstract
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  • Claims
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Problems solved by technology

However, since the myocardium is a nano-scale lamellar structure, traditio

Method used

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  • Natural protein/polycaprolactone nanofiber electrospun membrane, and preparation and application thereof
  • Natural protein/polycaprolactone nanofiber electrospun membrane, and preparation and application thereof
  • Natural protein/polycaprolactone nanofiber electrospun membrane, and preparation and application thereof

Examples

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Example Embodiment

[0036] Example 1

[0037] Using electrospun membranes containing natural protein components and synthetic degradable materials to construct a myocardial patch to repair infarcted myocardium:

[0038] 1. Natural protein / polycaprolactone nanofiber electrospun membrane and its construction:

[0039] A natural protein / polycaprolactone nanofiber electrospun membrane is prepared by mixing natural protein and polycaprolactone to prepare a spinning solution and electrospun. The natural protein is made of natural elastin and natural collagen combination. The natural protein / polycaprolactone nanofiber electrospun membrane is prepared by combining natural elastin (Elastin) (Shanghai Runyi Chemical Technology Co., Ltd., 9007-58-3, China) and natural collagen (Collagen I). ) (sigma, C9879, U.S.) was mixed into a natural protein mixture of 1:9, 2:8, and 3:7 by mass ratio. The natural protein mixture is mixed with the biodegradable polyester material polycaprolactone (PCL, (sigma, 440744-5G, Uni...

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Abstract

The invention provides a natural protein/polycaprolactone nanofiber electrospun membrane, and preparation and application thereof. The natural protein/polycaprolactone nanofiber electrospun membrane is obtained by mixing natural protein and polycaprolactone to prepare a spinning solution and performing electrospinning, and the natural protein comprises natural elastin and natural collagen. The natural protein containing cardiac muscle tissue extracellular matrix components is mixed with an artificially synthesized degradable material, and the nanofiber membrane can be prepared quickly and effectively through an electrostatic spinning technically. The natural protein/polycaprolactone nanofiber electrospun membrane has the advantages that preparation is simple, materials are easy to obtain, and preparation cost is low. The natural protein/polycaprolactone nanofiber electrospun membrane not only has a good biomechanical property, but also can fully simulate cardiac muscle tissue extracellular matrix environment. Biological simulation can be carried out in the aspects of the structure and the ingredient, and an effect of treating myocardial infarction by stem cell transplantation is improved. Therefore, the natural protein/polycaprolactone nanofiber electrospun membrane can serve as an extracellular culture medium to provide an extracellular matrix microenvironment for promoting cell adhesion, survival and multiplication.

Description

technical field [0001] The invention relates to the preparation of an electrospun membrane containing natural protein / PCL nanofiber components and its basic research and application in cell culture and tissue engineering, belonging to biomedicine, tissue engineering, cell biology, stem cell biology, biomaterials and Cardiovascular surgery field. Background technique [0002] Ischemic heart disease, as one of the main health problems in our country, has brought a heavy economic burden to our country and other countries in the world. Although the current drugs, interventional and surgical treatment of ischemic heart disease can effectively improve myocardial blood supply and save dying myocardium, they have no repair effect on non-functional myocardium that has caused death. Severe cardiac arrhythmias, heart failure, and cardiac arrest can result from massive myocardial death. For the treatment of advanced heart disease, the more effective method is heart transplantation. H...

Claims

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Application Information

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IPC IPC(8): A61L27/44A61L27/26A61L27/18D01D1/02D04H1/728
Inventor 陈瑶薛松刘洋谢静
Owner RENJI HOSPITAL AFFILIATED TO SHANGHAI JIAO TONG UNIV SCHOOL OF MEDICINE
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