Chondrocyte induction substrate for microfracture surgery and preparation method

Abstract

The invention provides a chondrocyte induction substrate for microfracture surgery and a preparation method. The chondrocyte induction substrate comprises gel and additives, the gel comprises fibrous protein, collagen, hyaluronic acid and thrombin, and the culture medium final comprises the additives of 233 micromoles-287 micromoles of vitamin C, 3.7 micromoles-8.9 micromoles of linoleic acid, 9 micromoles-17 micromoles of cholesterol, 6 nanomoles-15 nanomoles of dexamethasone, 43 micromoles-58 micromoles of acetylcysteine, 18 micrograms / mL-32 micrograms / mL of transferrin, 22 nanomoles-52 nanomoles of sodium selenite, 13 micromoles-24 micromoles of sodium pantothenate, 28 micromoles-43 micromoles of biotin, 7 micrograms / mL-18 micrograms / mL of insulin-like growth factors, 2 nanograms / mL-9 nanograms / mL of epidermal growth factors, 2 nanograms / mL-9 nanograms / mL of fibroblast growth factors, 2 nanograms / mL-9 nanograms / mL of platelet-derived growth factors, 30 nanograms / ml-70 nanograms / ml of transforming growth factors and 1% of insulin iron-selenium transducin.

Description

technical field [0001] The invention relates to the field of cartilage repair, in particular to a cartilage induction matrix for microfracture surgery and a preparation method. Background technique [0002] Articular cartilage damage, whether caused by trauma or pathology, cannot be repaired spontaneously, and usually leads to degenerative changes of cartilage, which in turn leads to osteoarthritis. At present, exogenous repair methods are mainly used, such as osteochondral transplantation and microfracture. In recent years, cartilage tissue engineering technology is considered to be the only way to repair large areas of cartilage damage, but the main method of cartilage tissue engineering is to cultivate seed cells in vitro to construct tissue engineered cartilage, which often requires two operations. Seed cells, tissue engineered cartilage transplantation for the second time, the in vitro construction period is as long as 2-3 weeks, the operation is difficult, the loss ra...

AI Technical Summary

Problems solved by technology

The advantages are one-time operation, minimally invasive, simple, economical, etc.; the disadvantages are that bone marrow cells are easily lost, large-scale damage cannot be repaired, and hyaline cartilage cannot be formed.

In fact, the cartilage defect repaired by microfracture surgery can only form fibrocartilage at the defect site due to the above reasons, and cannot restore the defect cartilage to its normal shape and function, which has a serious impact on the patient's quality of life

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