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Surface-enhanced Raman scattering base with visible hot spots, preparation method and method for detecting molecules through base

A surface-enhanced Raman and Raman scattering technology, applied in Raman scattering, material excitation analysis, etc., can solve the problems of uneven distribution of hot spots, large relative average deviation, and inability to meet the requirements of high-sensitivity qualitative analysis and quantitative analysis. achieve the effect of improving sensitivity

Active Publication Date: 2016-01-20
HEFEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the existing research, the characteristics of most substrates are: the distribution of "hot spots" is uneven, and techniques such as blindly looking for "hot spots" during detection make the distribution of enhancement factor values ​​​​of SERS detection results extremely wide, and the relative average deviation is relatively large. , it cannot meet the requirements of high-sensitivity qualitative analysis and quantitative analysis

Method used

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  • Surface-enhanced Raman scattering base with visible hot spots, preparation method and method for detecting molecules through base
  • Surface-enhanced Raman scattering base with visible hot spots, preparation method and method for detecting molecules through base
  • Surface-enhanced Raman scattering base with visible hot spots, preparation method and method for detecting molecules through base

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Step 1, preparation of silver nanowires

[0027] Add 10mL of ethylene glycol into a 100mL three-neck flask, heat it to reflux at 160°C for 2h, and inject 0.1M silver nitrate solution and 0.222g of polyvinylpyrrolidone (dissolved in 5mL of ethylene glycol solvent respectively) into it simultaneously with two syringe pumps. Middle), the injection rate was 0.2mL / min. Finally, react at a constant temperature of 160°C for 60 minutes. The synthesized silver nanowire sol is washed twice with acetone and secondary water according to the volume ratio of 1:9. The obtained silver nanowires have a length of 10 μm and a diameter of about 110 nm. .

[0028] Step 2, preparation of gold nanoparticles:

[0029] Add 90mL of secondary water and 1mL of 1% chloroauric acid solution into a 500mL three-neck flask, stir magnetically, heat the oil bath to boiling, then quickly add 1mL of 1% sodium citrate solution as a reducing agent , the diameter of the obtained gold nanoparticles is about...

Embodiment 2

[0035] Use the SERS substrate prepared by the present invention to measure 4-ATP molecules, and obtain SERS spectra and regional SERSmapping images:

[0036] Place the SERS substrate synthesized in Example 1 under a Raman optical microscope, find the position where the silver nanowire surface is modified with gold nanoparticles, that is, the position where the SERS "hot spot" is generated, and pipette 1 μL with a concentration of 10 -8 M's 4-ATP solution, slowly add dropwise to the "hot spot" or near it. After drying for about 1 minute, select the excitation light with a wavelength of 633nm and a laser power of 2mW, integrate for 1 second, detect the signal, and obtain the SERS spectrum of the 4-ATP molecule.

[0037] figure 2 A is the Raman optical microscope image of the SERS substrate prepared by the present invention, where the box marked is the spot range of the Raman laser: (4×4) square microns; figure 2 B is a schematic diagram when the substrate prepared by the pre...

Embodiment 3

[0040] Utilize the SERS substrate prepared by the present invention to measure cysteine, adenosine triphosphate, methamphetamine and paraoxon:

[0041] With the method identical with above-mentioned embodiment 2, can obtain respectively that concentration is 10 -6 M cysteine, 10 -6 M adenosine triphosphate, 10 -7 M of methamphetamine and 10 -8 SERS spectrum of paraoxon in M.

[0042] image 3 Medium, A: 10 -6 Cysteine ​​for M, B: 10 -6 ATP of M, C: 10 -7 Methamphetamine for M, D: 10 -8 M paraoxon, the results show that whether it is common cysteine ​​and adenosine triphosphate molecules in the human body, or common methamphetamine molecules and pesticide paraoxon molecules, the SERS substrate prepared by the invention can achieve high-sensitivity SERS Detection effect.

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Abstract

The invention relates to a surface-enhanced Raman scattering base with visible hot spots, a preparation method and a method for detecting molecules through the base. The SERS base is composed of a silver nanowire arranged on a Raman scattering substrate and gold nanoparticles modified on the surface of the silver nanowire. On the base, plasma resonance is formed between the silver nanowire and the gold nanoparticles, a strong electromagnetic field is generated, and therefore the visible hot spots are formed. The SERS base is placed under a Raman optical microscope, and the positions of the gold nanoparticles modified on the surface of the silver nanowire are SERS hot spots; the molecules to be detected are slowly dropwise added to the positions, and the molecules to be detected effectively fall into the SERS hot spot range. According to the preparation method, the gold nanoparticles are modified on the surface of the silver nanowire through the capillary force and gravity action simply. The SERS hot pots of the prepared base are visible, the molecules to be detected can effectively fall into the hot spot range, and the sensitivity of a detection result can be directly improved.

Description

technical field [0001] The invention relates to a surface-enhanced Raman scattering technology and a related detection method, in particular to a surface-enhanced Raman scattering substrate with visible hotspots, a preparation method and a method for detecting molecules using the substrate. Background technique [0002] Since Martin Fleischmann and his collaborators discovered the surface-enhanced Raman scattering (SERS) phenomenon in 1974, and Jeanmaire and Van Duyne two scientists gave a theoretical explanation of this amazing discovery in 1977, SERS technology has been developed for nearly 40 years. SERS technology, because it provides rich fingerprint information of molecules, can distinguish the structural differences of isomers, can distinguish the different orientations of the same molecule to be tested on the SERS substrate, and realizes highly sensitive detection, so it has been widely studied and Application, is an advanced analytical detection method. [0003] Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65B22F9/24
Inventor 吴义平李盼杨良保
Owner HEFEI UNIV
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