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Process for efficiently extracting l-valine from fermentation broth

A technology of valine and fermentation broth, which is applied in the direction of fermentation, microorganism-based methods, and the preparation of organic compounds, etc. It can solve the problems that cannot meet the requirements of amino acid production technology, high impurities in L-valine, and large production wastewater. problems, to achieve the effect of increased light transmittance and purity, improved fermentation efficiency, and low cost

Active Publication Date: 2018-09-14
XINJIANG FUFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The precipitation method is based on the specific combination of the precipitant and L-valine to form a precipitate, and then separate and extract. This method has the advantages of high yield and easy operation, but there is a technical problem of high environmental pollution; less, but the membrane filtration method can only remove a large amount of inorganic salt ions and macromolecular impurities, and the amino acids similar to L-valine in the fermentation broth cannot be eradicated, and the obtained L-valine has high impurities; The ion exchange method is to adjust the pH of the fermented liquid from which the bacteria have been removed to be acidic, use a strong acidic cation exchange resin to separate L-valine and impurity amino acids, and finally separate L-valine by elution with ammonia water
If the purity of L-valine is improved, it is necessary to adopt multi-stage ion exchange columns for serial extraction, which will lead to a large amount of production wastewater in the production process, and the extraction yield by ion exchange method is also low
At present, a single extraction method can no longer meet the requirements of amino acid production technology, and a more economical and effective combination method is sought to solve this technical problem

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] A process for efficiently extracting L-valine from a fermented liquid, comprising the steps of:

[0028] 1) According to routine cultivation of three strains to obtain seed liquid, the concentration of the seed liquid of the three strains is 1 × 10 8 Individual / ml, mix Brevibacterium lactofermentum ATCC13869 seed liquid, Corynebacterium glutamicum ATCC21670 seed liquid and Bacillus subtilis ATCC21616 seed liquid according to the volume ratio of 2:1:1, and then insert the fermentation culture according to the inoculum size of 5%. The fermented medium was continuously fermented for 60 hours to obtain a fermented liquid; during the fermenting process, the temperature was controlled at 30°C, the pH was controlled at 6.6, and the glucose concentration was not lower than 30g / L;

[0029] The components of the fermentation medium are: glucose 50g / L, corn steep liquor 30g / L, soybean meal 10g / L, lactose 10g / L, ammonium sulfate 6g / L, potassium dihydrogen phosphate 0.1g / L, magnesiu...

Embodiment 2

[0039] Embodiment 1 fermentation efficiency of the present invention:

[0040] Using liquid chromatography to detect the valine content in the fermentation broth, the average L-valine content is 8.3%; using a single Brevibacterium lactofermentum or Corynebacterium glutamicum fermentation is 4.9% or 3.2%; the fermentation efficiency is greatly improved Improvement; the combined fermentation of Brevibacterium lactofermentum and Corynebacterium glutamicum was 7.1%.

[0041] Extraction efficiency:

[0042] The extraction yield of L-valine in the present invention is about 68%, and the purity reaches 99% through HPLC detection, reaching the pharmaceutical grade standard; while the extraction yield of L-valine in the traditional process is about 60%, and the purity is only 95% through HPLC detection. %about.

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Abstract

The invention belongs to the field of amino acid extraction, and relates to a process for efficiently extracting L-valine from fermentation liquor. The process includes steps of 1), carrying out fermentation; 2), carrying filtering by the aid of micro-filtration membranes; 3), carrying evaporation concentration; 4), carrying out crystallization; 5), carrying dissolving; 6), exchanging ions; 7), carrying out filtering by the aid of ultra-filtration membranes; 8), carrying vacuum concentration; 9), carrying centrifugal drying. Compared with the traditional process, the process has the advantages that the process is implemented by the aid of organic micro-filtration membrane and organic ultra-filtration membrane systems, and the organic membranes are good in flexibility, high in separation selectivity, easy to shape and low in cost as compared with inorganic membranes; consumption of steam and concentrate water can be reduced by the four-effect concentration process as compared with single-effect concentration evaporators, accordingly, the production cost can be reduced, the production efficiency can be improved, and the like.

Description

technical field [0001] The invention relates to a process for efficiently extracting L-valine from fermentation liquid, belonging to the field of amino acid extraction process. Background technique [0002] L-valine (L-val), scientific name 2-amino-3-methylbutyric acid, is one of the eight essential amino acids for the human body. It is collectively called branched-chain amino acids together with L-leucine and L-isoleucine. It is widely used in medicine, seasoning, nutritional additives, feed additives, etc. [0003] At present, the extraction methods of L-valine include precipitation method, whole membrane method and ion exchange method. The precipitation method is based on the specific combination of the precipitant and L-valine to form a precipitate, and then separate and extract. This method has the advantages of high yield and easy operation, but there is a technical problem of high environmental pollution; less, but the membrane filtration method can only remove a la...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P39/00C12P13/08C07C229/08C07C227/40C12R1/125C12R1/15C12R1/13
Inventor 唐永强杨瑞丽沈伟伟李学朋张修军
Owner XINJIANG FUFENG BIOTECH