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Method for determination of contents of oleanolic acid and ursolic acid in kiwi fruit oil through high performance liquid chromatography

A high-performance liquid chromatography and oleanolic acid technology, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of lack of specificity and inability to chemically synthesize, and achieve extraction time saving, good linear relationship, and high sensitivity Effect

Inactive Publication Date: 2016-02-03
查孝柱
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  • Application Information

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Problems solved by technology

[0004] At present, these two substances can only be extracted from plants and cannot be chemically synthesized; the detection methods include spectrophotometry, thin-layer scanning and high-performance liquid chromatography. , spectrophotometry usually measures the total amount of triterpenoids, and the method lacks specificity; while ordinary thin-layer chromatography is difficult to separate OA and UA; in comparison, HPLC is easier to implement the separation of the two, and ultraviolet detection Better reproducibility than evaporative light scattering detection

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  • Method for determination of contents of oleanolic acid and ursolic acid in kiwi fruit oil through high performance liquid chromatography
  • Method for determination of contents of oleanolic acid and ursolic acid in kiwi fruit oil through high performance liquid chromatography

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Embodiment 1

[0015] Embodiment 1: the method for the determination of oleanolic acid, ursolic acid content in kiwi fruit oil by high performance liquid chromatography may further comprise the steps:

[0016] (1) Preparation of Reference Substance Solution Accurately weigh 10.20 mg of oleanolic acid reference substance and 8.54 mg of ursolic acid reference substance, put them in 10ml measuring bottles respectively, add methanol to dissolve and dilute to the scale, shake well, and obtain Oleanolic Acid Fruit acid, ursolic acid reference substance stock solution.

[0017] (2) Treatment of the sample solution: Take about 0.5-1.5 g of the kiwi oil sample, accurately weigh it, put it in a stoppered Erlenmeyer flask, add 25 ml of methanol precisely, seal it tightly, weigh it, and extract it at 60-90 ° C for 1 -4h, sonicate for 5-10min, weigh again, make up the lost weight with methanol, shake well, filter, and take the filtrate to obtain the final product;

[0018] (3) After the filtrate is pass...

Embodiment 2

[0021] Embodiment 2: concrete experimental data of the present invention:

[0022] 1 Instruments and reagents

[0023] Instrument: 1100 high performance liquid chromatography (including G1311A pump, G1322 degasser, G1313AZ autosampler, G1313A column thermostat, G1315DAD; Agilent1100ChemStation); BP221D electronic balance (Sartorius company); KQ-250DB CNC ultrasonic cleaner ( Kunshan Ultrasonic Instrument Co., Ltd.); quartz sub-boiling high-purity water extractor (Jiangsu Jincheng Teaching Instrument Factory).

[0024] Reagents: Oleanolic acid and ursolic acid reference substances (110709-200505, 110742-200516, for content determination) are provided by China Institute for the Control of Pharmaceutical and Biological Products; Co., Ltd. Jusheng brand wild kiwi oil) and other reagents were of analytical grade.

[0025] Mobile phase preparation: The mobile phase was prepared according to the proportions of 265mL methanol, 35mL water, 0.05mL glacial acetic acid and 0.05mL trieth...

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Abstract

The invention discloses a method for determination of contents of oleanolic acid and ursolic acid in kiwi fruit oil through the high performance liquid chromatography. The method is mainly characterized in that the reversed phase high performance liquid chromatography is adopted, wherein chromatographic conditions are as follows: a, a C18 column with the parameters of 250*4.6mm and 5[mu]m is used as a chromatographic column; b, a mobile phase comprises 265% of methanol, 35% of water, 0.1% of glacial acetic acid and 0.05% of triethylamine and the mobile phase is prepared from 265mL of methanol, 35mL of water, 0.05mL of glacial acetic acid and 0.05mL of triethylamine; c, the detection wavelength of an ultraviolet detector is 210nm; d, the flow velocity is 0.6mL / min, the sampling volume is 20[mu]L and the column temperature is 20 DEG C; treatment of a sample solution comprises the following steps: approximately 1.0-2.5g of kiwi fruit oil sample is taken, precise weighing is carried out, the kiwi fruit oil sample is put into a conical flask with a plug, 25ml of methanol is added precisely, the conical flask is sealed with the plug, weighing is carried out, soxhlet extraction is carried out for 1-4 h at 60-90 DEG C, ultrasonic treatment is carried out for 5-10 min, weighing is carried out again, the weight loss is complemented through methanol, uniform shaking and filtering are carried out, and filtrate is taken continuously, so that the contents of oleanolic acid and ursolic acid in kiwi fruit oil are obtained. The quality is effectively controlled. The method disclosed by the invention is sensitive, efficient, accurate and stable, and can effectively control the contents of oleanolic acid and ursolic acid in the kiwi fruit oil.

Description

technical field [0001] The present invention relates to a method for measuring the content of oleanolic acid and ursolic acid in kiwi fruit oil by high performance liquid chromatography. It belongs to the technical field: 2. Biology and new medical technology (7) Modern agricultural technology Storage and transportation: technologies such as agricultural product quality and safety evaluation, rapid detection, and whole-process quality control. Background technique [0002] HPLC is a high-pressure, high-efficiency, and high-sensitivity separation technology that can be used to separate and analyze organic compounds with high boiling points, large relative molecular weights, and poor thermal stability. It is widely used in chemistry, chemical engineering, polymers, biology, food, medicine and other fields. [0003] Oleanolic acid and ursolic acid mainly exist in kiwi fruit oil and its oil foot, and are pentacyclic triterpenoids; they are similar in nature and difficult to sepa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 查孝柱
Owner 查孝柱