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Bacillus amyloliquefaciens LX-J1 and application thereof

A technology for dissolving starch spores and LX-J1, which is applied in the field of microorganisms and can solve problems such as easy residues and damage to the ecological environment.

Inactive Publication Date: 2016-02-10
领先生物农业股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition to optimizing seeds and crop rotation, the current prevention and control measures for peanut blight are all chemical pesticides, which not only damage the ecological environment but also tend to remain

Method used

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  • Bacillus amyloliquefaciens LX-J1 and application thereof
  • Bacillus amyloliquefaciens LX-J1 and application thereof
  • Bacillus amyloliquefaciens LX-J1 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0096] Embodiment 1: the cultivation of bacillus amyloliquefaciens LX-J1

[0097] The implementation steps of this embodiment are as follows:

[0098] A. Incline cultivation

[0099] Prepare slant medium: dissolve 5g beef extract, 2.5g sodium chloride, 5g peptone and 20g agar in water, then add water to a total volume of 1000ml, then use 0.8N hydrochloric acid or sodium hydroxide aqueous solution to adjust the pH value of the obtained solution to pH7.0.

[0100] Use an inoculation loop to pick a ring of Bacillus amyloliquefaciens LX-J1 stored in a glycerol tube to inoculate on the slant medium slant, and then place it in a constant temperature incubator and culture it on the slant for 48 hours at a temperature of 28°C;

[0101] B. Seed cultivation

[0102] Preparation of liquid seed culture medium: 7g beef extract, 3g sodium chloride and 5g peptone were dissolved in water, then supplemented with water to a total volume of 1000ml, then using 0.8N hydrochloric acid or sodium hy...

Embodiment 2

[0107] Embodiment 2: the cultivation of Bacillus amyloliquefaciens LX-J1

[0108] The implementation steps of this embodiment are as follows:

[0109] A. Incline cultivation

[0110] Prepare slant medium: dissolve 7g of beef extract, 3.4g of sodium chloride, 10g of peptone and 20g of agar in water, add water to a total volume of 1000ml, and then use 0.1N hydrochloric acid or potassium hydroxide aqueous solution to adjust the pH value of the obtained solution to pH7.1.

[0111] Use an inoculation loop to pick a ring of Bacillus amyloliquefaciens LX-J1 stored in a glycerol tube and inoculate it on the slant medium slant, and then place it in a constant temperature incubator and culture it on the slant for 40 hours at a temperature of 29°C;

[0112] B. Seed cultivation

[0113] Preparation of liquid seed medium: Dissolve 8g of beef extract, 2.4g of sodium chloride and 10g of peptone in water, then add water to a total volume of 1000ml, then use 0.1N hydrochloric acid or potass...

Embodiment 3

[0118] Embodiment 3: the cultivation of Bacillus amyloliquefaciens LX-J1

[0119] The implementation steps of this embodiment are as follows:

[0120] A. Incline cultivation

[0121] Prepare slant medium: Dissolve 8g of beef extract, 2.0g of sodium chloride, 7g of peptone and 20g of agar in water, add water to a total volume of 1000ml, and then use 2.0N phosphoric acid or ammonia solution to adjust the pH of the resulting solution to pH7 .2.

[0122] Use an inoculation loop to pick a ring of Bacillus amyloliquefaciens LX-J1 stored in a glycerol tube to inoculate on the slant medium slant, and then place it in a constant temperature incubator and culture it on the slant for 44 hours at a temperature of 30°C;

[0123] B. Seed cultivation

[0124] Preparation of liquid seed medium: Dissolve 5g of beef extract, 3.6g of sodium chloride and 7g of peptone in water, add water to a total volume of 1000ml, and then use 2.0N phosphoric acid or ammonia solution to adjust the pH of the ...

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Abstract

The invention relates to Bacillus amyloliquefaciens LX-J1 and application thereof. A preparation of the Bacillus amyloliquefaciens LX-J1 is prepared from a fermentation solution, copper sulfate, a phosphorus compound, a potassium compound and xanthan gum. Compared with the prior art, the liquid preparation of Bacillus amyloliquefaciens LX-J1 disclosed by the invention has the advantages that the inhibition ratio for rhizoctonia cerealis is up to 87.5%, the inhibition ratio for rhizoctonia solani is up to 78.82%, the inhibition ratio for pythium ultinum trow is up to 87%, the inhibition ratio for sclerotium rolfsii is up to 80.67%, the inhibition ratio for potato black scurf is up to 78.57%, and the inhibition ratio for corticium rolfsii saccardo can be up to more than 83%.

Description

【Technical field】 [0001] The invention relates to the technical field of microorganisms. More specifically, the present invention relates to a strain of Bacillus amyloliquefaciens LX-J1, and also relates to the application of said Bacillus amyloliquefaciens LX-J1. 【Background technique】 [0002] Peanut is a leguminous crop, also known as "groundnut" or "longevity fruit", with an oil content of up to 50%. It is one of the main oil crops. Peanut canker sore is one of the important diseases that occur on peanuts. After being infected by the roots, pods and stem bases, it is initially brown and soft rot. Flowers often form at the base of the stem near the ground and on the soil surface nearby. White silk with rapeseed-like sclerotia, stems and leaves turn yellow, gradually wither, peanut pods rot. The pathogen begins to germinate under high temperature and high humidity conditions, infecting peanuts, sandy soil, continuous heavy cropping, excessive density and no ventilation,...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/07
Inventor 杨娜李丽艳杜迎辉徐志文
Owner 领先生物农业股份有限公司
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