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Nanometer photo-thermal therapy reagent and preparing method thereof

A thermal treatment and nano-light technology, which can be used in pharmaceutical formulations, medical preparations with non-active ingredients, and medical preparations containing active ingredients, etc. It can solve the problems of limited application, low enrichment, and unstable near-infrared dyes. , to achieve long-term stability

Active Publication Date: 2016-02-17
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although near-infrared dyes have the potential to be applied in photothermal therapy, pure near-infrared dyes still have some defects: first, most near-infrared dyes are unstable in aqueous phase and exhibit concentration-dependent aggregation; Under the irradiation of infrared light, the photobleaching effect of dye molecules makes its photothermal stability unguaranteed; while some dye molecules are less enriched in tumor sites during photothermal therapy, resulting in the effect of tumor photothermal ablation. Greatly reduced
However, due to the charge characteristics of the surface of polyaniline, it is positively charged in most cases, which will significantly affect its biocompatibility, which limits its further application in the human body.

Method used

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  • Nanometer photo-thermal therapy reagent and preparing method thereof
  • Nanometer photo-thermal therapy reagent and preparing method thereof
  • Nanometer photo-thermal therapy reagent and preparing method thereof

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preparation example Construction

[0024] The embodiment of the present invention also provides a preparation method of the above-mentioned nano-photothermal therapy reagent, which includes the following steps:

[0025] S01: Dissolve monomethoxy polyethylene glycol-polylactic acid-glycolic acid block copolymer (mPEG-PLGA) and / or polyethylene glycol-polylactic acid-glycolic acid block copolymer (PEG-PLGA) in In an organic solvent, a polymer solution is obtained;

[0026] S02: adding a porphyrin compound capable of reacting with hydroxyl groups on the polymer to the polymer solution; or adding a catalyst, stirring and reacting to obtain a modified porphyrin polymer;

[0027] S03: preparing the modified porphyrin polymer into nanoparticles; preferably, the size of the nanoparticles is 50-1000 nm.

[0028] In the above-mentioned preparation method:

[0029] In step S01, the organic solvent can be an organic solvent capable of dissolving mPEG-PLGA or PEG-PLGA; preferably dichloromethane, chloroform, N,N-dimethylfo...

Embodiment 1

[0040] A nano-photothermal therapy agent, which is a nanoparticle formed by a polymer covalently bonded to a monomethoxy polyethylene glycol-polylactic acid-glycolic acid block copolymer (mPEG-PLGA) and a porphyrin compound .

[0041] The preparation method of the nano photothermal therapy reagent comprises:

[0042] 1) Dissolving 100nmol of mPEG-PLGA in 10mL of anhydrous dichloromethane to obtain a polymer solution;

[0043] 2) Add 50nmol of protoporphyrin, 50nmol of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide, 25nmol of 4-(dimethylamino)pyridine and 50 μL of N,N-diisopropylethylamine was reacted at room temperature for 48 hours under the protection of argon; the resulting product was filtered, dialyzed for 24 hours, and freeze-dried to obtain mPEG-PLGA-porphyrin;

[0044] 3) Dissolve the obtained mPEG-PLGA-porphyrin in dichloromethane, the concentration of mPEG-PLGA-porphyrin is 20mg / mL, sonicate for 15min until completely dissolved; take 0.2mL deionized water and 1.0mL ...

Embodiment 2

[0046] A nano-photothermal therapy agent, which is a nanoparticle formed by a polymer covalently bonded to a monomethoxy polyethylene glycol-polylactic acid-glycolic acid block copolymer (mPEG-PLGA) and a porphyrin compound .

[0047] The preparation method of the nano photothermal therapy reagent comprises:

[0048] 100 nmol of mPEG-PLGA, 100 nmol of chlorin e6, 50 nmol of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide, 25 nmol of 4-(dimethylamino)pyridine and 50 μL of triethylamine in 10 mL of anhydrous Mix in dichloromethane, react at room temperature for 48 hours under the protection of argon, dialyze the product for 48 hours, and freeze-dry to obtain mPEG-PLGA-chlorin e6.

[0049] Dissolve 7.5 mg of mPEG-PLGA-chlorin e6 in 1.5 mL of acetone and sonicate for 15 min until completely dissolved. The above solution was added dropwise into 10 mL of deionized water at a rate of 30 μL / min under magnetic stirring. Stirring was continued for 30 min to solidify the nanoparticles. ...

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Abstract

The invention provides a nanometer photo-thermal therapy reagent and a preparing method thereof. The nanometer photo-thermal therapy reagent is nano-particles formed by a polymer obtained through covalent bond connection of mPEG-PLGA and / or PEG-PLGA and porphyrins, and preferably, the size of the nano-particles is 50-1000 nm. The preparing method comprises the steps of preparing the polymer for modifying porphyrins, and then preparing the nano-particles. The nanometer photo-thermal therapy reagent has certain application potential in photo-thermal therapy reagents.

Description

technical field [0001] The invention belongs to the field of nano-biomedicine, and in particular relates to a nano-photothermal therapy reagent and a preparation method thereof. Background technique [0002] Hyperthermia is a method that uses heat to treat tumors. Hyperthermia for cancer has many advantages over traditional cancer treatments. Most hyperthermia methods are non-invasive, cause little damage to the patient, are simple and easy to operate, and are suitable for some internal tumors in important organs that are not suitable for surgery. It utilizes the absorption of near-infrared light by a special photothermotherapy agent, and converts the absorbed light energy into heat to increase the temperature of the tumor site, thereby inducing apoptosis or producing a direct lethal effect on cells. Compared with traditional surgical treatment methods, systemic toxicity is greatly reduced. Therefore, photothermal therapy is regarded as one of the technologies with great p...

Claims

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Application Information

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IPC IPC(8): A61K41/00A61K47/48A61K9/14A61P35/00
Inventor 吴雁聂广军苏世帅邵磊厚
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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