Silk fibroin/octacalcium phosphate composite bone scaffold material and preparation method

A technology of silk fibroin and octacalcium phosphate, applied in the field of materials, to achieve good degradation performance and mechanical properties, increase strength, and enhance osteogenic performance

Active Publication Date: 2016-03-02
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, in the prior art, there is no porous scaffold prepared by silk

Method used

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  • Silk fibroin/octacalcium phosphate composite bone scaffold material and preparation method
  • Silk fibroin/octacalcium phosphate composite bone scaffold material and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] 1. Dissolve silk fibroin in 50 mL of calcium nitrate 40 mM solution to prepare a solution with a silk fibroin concentration of 0.1 wt%.

[0029] 2. Prepare 50 mL of sodium dihydrogen phosphate solution with a concentration of 40 mM.

[0030] 3. Slowly add the silk fibroin-containing calcium nitrate solution prepared in step 1) to the sodium dihydrogen phosphate solution prepared in step 2) under stirring conditions at 68°C, and continue stirring for 1 hour to finally obtain a white precipitate things. After the white precipitate was centrifuged and washed three times, it was placed at -80°C for 1 day, and after 2 days of freeze-drying, the silk fibroin / octacalcium phosphate complex was obtained. Its infrared spectrum is as follows: figure 1 shown;

[0031] 4. Grind the silk fibroin / octacalcium phosphate compound obtained in step 3) into powder with a mortar, weigh 20 mg of powder, mix it with 1 mL of silk fibroin solution with a concentration of 6 wt%, and inject it i...

Embodiment 2

[0034] 1. Dissolve silk fibroin in 50 mL of calcium nitrate 80 mM solution to prepare a solution with a silk fibroin concentration of 0.5 wt%.

[0035] 2. Prepare 50 mL of sodium dihydrogen phosphate solution with a concentration of 80 mM.

[0036] 3. Slowly add the silk fibroin-containing calcium nitrate solution prepared in step 1) to the sodium dihydrogen phosphate solution prepared in step 2) under stirring conditions at 70°C, and continue to stir for 1.5 hours to finally obtain a white precipitate things. The white precipitate was centrifuged and washed three times, placed at -80°C for 1 day, and freeze-dried for 2 days to obtain the silk fibroin / octacalcium phosphate complex;

[0037] 4. Grind the silk fibroin / octacalcium phosphate compound obtained in step 3) into powder with a mortar, weigh 50 mg of powder, mix it with 1 mL of silk fibroin solution with a concentration of 10 wt%, and inject it into a cylindrical mold. Placed at -80°C for 1 day and freeze-dried for 2 ...

Embodiment 3

[0040] 1. Dissolve silk fibroin in 120 mL of calcium nitrate 40 mM solution to prepare a solution with a silk fibroin concentration of 2 wt%.

[0041] 2. Prepare 120 mL of sodium dihydrogen phosphate solution with a concentration of 40 mM.

[0042] 3. Under the condition of heating and stirring at 68°C, slowly add the calcium nitrate solution containing silk fibroin prepared in step 1) to the sodium dihydrogen phosphate solution prepared in step 2), and continue stirring for 1.5 hours, and finally a white precipitate is obtained things. The white precipitate was centrifuged and washed three times, placed at -80°C for 1 day, and freeze-dried for 2 days to obtain the silk fibroin / octacalcium phosphate complex;

[0043] 4. Grind the silk fibroin / octacalcium phosphate compound obtained in step 3) into powder with a mortar, weigh 100 mg of powder, mix it with 1 mL of silk fibroin solution with a concentration of 10 wt%, and inject it into a cylindrical mold. Placed at -80°C for 1...

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Abstract

The invention relates to the technical field of materials, and discloses a silk fibroin/octacalcium phosphate composite bone scaffold material. The silk fibroin/octacalcium phosphate composite bone scaffold material is prepared from silk fibroin and octacalcium phosphate, wherein the mass ratio of silk fibroin to octacalcium phosphate is 10:1-10. A preparation method includes the steps that a sodium dihydrogen phosphate solution and a calcium nitrate solution containing silk fibroin are prepared, wherein the concentration of the sodium dihydrogen phosphate solution is equal to that of the calcium nitrate solution; the two solutions are mixed with heating and stirring to obtain white precipitate, and a fibroin/octacalcium phosphate composite is obtained after centrifugal washing and freeze-drying; the fibroin/octacalcium phosphate composite and a high-concentration silk fibroin solution are evenly mixed, the mixture is injected into a mould, and a porous scaffold is obtained after freeze-drying; the silk fibroin/octacalcium phosphate composite bone scaffold material is obtained after aftertreatment. The prepared silk fibroin/octacalcium phosphate composite bone scaffold material has good biocompatibility, degradability, mechanical performance and osteogenesis performance, is easy to prepare and can be applied to the field of bone tissue engineering.

Description

technical field [0001] The invention relates to the technical field of materials, in particular to a silk fibroin / octacalcium phosphate composite bone scaffold material and a preparation method thereof. Background technique [0002] From the perspective of cells, scaffolds and active factors, bone tissue engineering technology relies on biomaterials to induce bone tissue regeneration and in situ bone repair. However, the repair effect of bone tissue engineering scaffold materials is still different from that of autologous bone. From the perspective of bionics, the preparation of artificial bone graft materials that simulate the composition and structure of natural bone is an important research content of bone repair materials. Phosphate ceramic materials can form a strong bone bond with bone, and have good biocompatibility and bioactivity, so they are ideal substitute materials for bone tissue in clinical practice. Hydroxyapatite is the most abundant inorganic component in...

Claims

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Application Information

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IPC IPC(8): A61L27/56A61L27/46A61L27/22A61L27/50
CPCA61L27/365A61L27/3691A61L27/46A61L27/50A61L27/56A61L2400/18A61L2430/02C08L89/00
Inventor 韩凤选李斌龚嘉伟过倩萍朱彩虹杨惠林
Owner SUZHOU UNIV
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