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A collagen-based cartilage scaffold

A collagen and cartilage technology, applied in medical science, prostheses, tissue regeneration, etc., can solve the problems of back layer separation, poor biocompatibility, cartilage ossification, etc., to achieve increased mechanical properties, complete double-layer structure, The effect of tight connection

Active Publication Date: 2018-08-21
ZHEJIANG XINGYUE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Most of the current applications are polymer materials or collagen sponges and other scaffolds to transfer chondrocytes to repair cartilage defects, but medical polymer materials have poor biocompatibility and their degradation products are destructive to tissues, while single-layer collagen sponges have poor mechanical properties and The repaired cartilage defect may lead to cartilage ossification. The current preparation method of multi-layer structure has the defect of unstable structure and shape, which may easily cause separation between layers after implantation in the body.

Method used

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  • A collagen-based cartilage scaffold
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  • A collagen-based cartilage scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] (1) Spread the collagen solution with a concentration of 20mg / ml into a polytetrafluoroethylene grooved mold with a size of 100mm×100mm×5mm, and freeze at -20°C for 16 hours to form.

[0023] (2) Immerse the frozen collagen into a phosphate buffer solution (disodium hydrogen phosphate (Na 2 HPO 4 ) 2.436g / L and potassium dihydrogen phosphate (KH 2 PO 4 ) 0.8g / L, adjust the pH to 7.4) for 24 hours.

[0024] (3) Extrude the dehydrated collagen as a whole with gradient pressure (70-80Kg for 30 seconds, 100-110Kg for 30 seconds, 140-150Kg for 30 seconds, 200-210Kg for 60 seconds) to form a film, and then spread the concentration on the dense film. 5mg / ml collagen solution to 5mm thickness.

[0025] (4) The whole double-layer collagen was frozen at -30°C for 16 hours, and then dried in a freeze dryer for 48 hours.

[0026] (5) The freeze-dried bilayer collagen was cross-linked with EDC-NHS (EDC and NHS concentrations were 20 mg / ml and 5 mg / ml, respectively) for 3 hours....

Embodiment 2

[0030] (1) Spread the collagen solution with a concentration of 5mg / ml into a polytetrafluoroethylene grooved mold with a size of 100mm×100mm×3mm, and freeze at -80°C for 12 hours to form.

[0031] (2) Immerse the frozen collagen in citric acid-disodium hydrogen phosphate buffer solution containing 50g / L NaCl concentration (containing citric acid 1.92g / L and disodium hydrogen phosphate 25.8g / L, adjust the pH to 8.0) 12 hours.

[0032] (3) Extrude the dehydrated collagen as a whole with gradient pressure (50-70Kg for 30 seconds, 100-110Kg for 30 seconds, 150-170Kg for 30 seconds, 200-250Kg for 30 seconds) to form a film, and spread the concentration on the dense film. 5mg / ml collagen solution to 3mm thickness.

[0033] (4) The whole double-layer collagen was frozen at -20°C for 8 hours, and then dried in a freeze dryer for 12 hours.

[0034] (5) The lyophilized bilayer collagen was cross-linked with glutaraldehyde at a concentration of 0.05% (w / v) for 8 hours.

[0035] (6) W...

Embodiment 3

[0038] (1) Spread the collagen solution with a concentration of 40mg / ml into a polytetrafluoroethylene grooved mold with a size of 100mm×100mm×8mm, and freeze at -30°C for 18 hours to form.

[0039] (2) The frozen collagen was immersed in Tris-hydrochloric acid buffer solution (containing Tris 6.057 g / L and 0.042 mol / L hydrochloric acid, pH adjusted to 7.0) containing KCl concentration 340 g / L for 24 hours.

[0040] (3) Extrude the whole dehydrated collagen with gradient pressure (80-100Kg for 30 seconds, 120-150Kg for 30 seconds, 180-200Kg for 30 seconds, 400-500Kg for 30 seconds) to form a film, and then spread it on the dense film with a concentration of 20mg / ml collagen solution to 8mm thickness.

[0041] (4) The whole double-layer collagen was frozen at -80°C for 20 hours, and then dried in a freeze dryer for 48 hours.

[0042] (5) The freeze-dried bilayer collagen was cross-linked with genipin at a concentration of 1 mmol / L for 6 hours.

[0043] (6) Wash the cross-link...

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Abstract

The invention discloses a collagen-based cartilage scaffold. The cartilage scaffold is prepared by the following steps: (a) freezing and molding a tiled collagen solution, soaking in a hypertonic buffer solution, dehydrating, and extruding to form a film; (b) laying a layer of the collagen solution on the surface of the film, and freeze-drying; (c) integrally crosslinking by using a crosslinking agent, washing, and freeze-drying to obtain the collagen-based cartilage scaffold. According to the collagen-based cartilage scaffold, natural transition and seamless connection between the compact layer and a porous loose layer can be realized, so that the two-layer structure can be complete, the connection between the two layers can be compact, the mechanical performance of the scaffold can be improved, and two layers of the scaffold can be effectively prevented from being separated after the scaffold is implanted into a human body.

Description

technical field [0001] The invention relates to medical biomaterial technology, in particular to a collagen-based cartilage scaffold. Background technique [0002] Articular cartilage injury is a common clinical disease, which is caused by sports trauma, traffic accidents and various arthritis diseases. Cartilage is difficult to repair once damaged. At present, the commonly used clinical methods for the treatment of cartilage defects include microfracture, autologous non-weight-bearing cartilage transplantation or allogeneic cartilage transplantation, and autologous chondrocyte transplantation techniques, but each has its own shortcomings. For example, microfracture usually forms fibrocartilage tissue. , the long-term repair effect is not ideal; autologous non-weight-bearing cartilage transplantation causes secondary damage, and allogeneic cartilage transplantation has the risk of immune rejection and disease transmission; autologous chondrocyte transplantation requires a s...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/40A61L27/56
CPCA61L27/24A61L27/56A61L2430/06C08L89/00
Inventor 徐国伟赵洪石马南
Owner ZHEJIANG XINGYUE BIOTECH
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