A kind of arbuscular mycorrhizal fungal agent
A technology of arbuscular mycorrhizal fungi and bacterial agents, applied in the field of microbial bacterial agents, can solve the problems of high technical content, poor quality of bacterial agents, and restrictions on the application of AM fungi, and achieve high infection rates
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[0023] (1), germination:
[0024] Host plant selects white clover (scientific name: Trifolium repens L) and Ophiopogon japonicus (scientific name: Ophiopogon japonicus (Linn.f.) Ker‐Gawl.), the host plant seeds (white clover seed and Ophiopogon japonicus seed) in 5% sodium hypochlorite Soak in the solution for 15 minutes, take it out, blot it dry with absorbent paper, rinse it with sterile water ten times, place it on a sterile filter paper moistened with sterile water in a sterile petri dish, and incubate in dark at 28°C until the seeds germinate.
[0025] (2) Preparation of matrix and culture container:
[0026] After passing the construction river sand through a 1mm sieve, it is fully mixed with soil (ordinary farmland soil) at a mass ratio of 3:1, and used as a culture substrate for the growth of host plants and the proliferation of AM fungi;
[0027] Wipe the inside and outside of the culture container (paper cup or small flowerpot) with 75% alcohol three times, and then...
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