Method for pre-treating veterinary drug residual quantity in detection of animal tissue

Abstract

The invention belongs to the field of analytical chemistry, and relates to a method for pre-treating veterinary drug residual quantity in detection of animal tissue. The method comprises the following steps: adding a proper amount of a buffer in the homogenized animal tissue for adjusting the pH value to weak acidic, adding enough amount of an enzymatic hydrolysis solution, then performing constant temperature water-bath or infrared auxiliary microwave processing enzymatic hydrolysis incubation, centrifuging the solution after incubation is carried out, washing by an organic solvent for deposition, centrifuging the materials and collecting the organic solvent for merging with a supernatant, performing rotary evaporation on the solution at room temperature to remove the solvent, using a few organic solvent for re-dissolving the residue to obtain a sample matrix as a functionalization composite material which can effectively enrich the microscale veterinary drug residue in the animal tissue, and conveniently performing subsequent detection. The method has the advantages of simple and easy operation, short time consuming, low cost, and low cost of equipment, and the method is suitable for all animal tissue.

Description

technical field [0001] The invention belongs to the field of pharmaceutical analysis and relates to a pretreatment method for detecting veterinary drug residues in animal tissues. The method can be used to eliminate the interference of complex endogenous substances in animal tissues and effectively enrich trace veterinary drug residues. Background technique [0002] With the development of science and technology and medicine, the harm of veterinary drug residues in animal source food to human body has been gradually recognized by people and has aroused social concern. According to reports, China and the European Union have banned the illegal addition of feed and issued regulations A series of related regulations. However, due to the complexity of the animal tissue itself, the serious matrix effect, and the lack of an effective enrichment method for extremely small amounts of veterinary drug residues in the specific detection, the research encountered many bottlenecks, and it...

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Problems solved by technology

[0006] The purpose of the present invention is to provide a method for detecting veterinary drug residues in animal tissues in order to solve the problems of the existing technology in the analysis of veterinary drug residues in animal tissues that the influence of the matrix effect is too large and the traces of veterinary drugs cannot be effectively enriched. pre-processing method

[0007] In the method of the present invention, microwave assistance or infrared are combined with nanomaterials, which can not only solve the problems of too long time and cumbersome process, but also use the advantages of nanomaterials as adsorbents, which not only have the super large specific surface area of ​​mesoporous molecular sieves, but also have an orderly The size of the pores and the unique modification group allow them to enter the pores and combine with the hydrophobic force between the veterinary drug residue and the alkyl group, while the macromolecular protein is excluded from the pores, so that it can treat the pesticide residues in complex biological samples Drugs can be effectively enriched, thereby simplifying the analysis difficulties of trace veterinary drug residues, and providing a new way for the application of composite nanomaterials

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