Chicken embryo peptide as well as preparation method and application thereof in preparation of anti-inflammation, acne removal and black hair generation products
A chicken embryo peptide and product technology, which is applied in the biological field to achieve the effects of promoting regeneration, improving extraction efficiency and being easy to operate.
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Embodiment 1
[0062] (1) Hatching chicken embryos: Take SPF grade fertilized eggs and put them into the incubator for incubation. The incubation temperature is controlled at 37.5°C, the relative humidity is 60%, and the eggs are automatically turned every 2 hours. After hatching to 16 days, take out the fertilized eggs , put it in a freezer at -20°C, stop the incubation and save it for later use.
[0063] (2) Homogenization and cell crushing: surface disinfection of the hatched eggs, removal of egg shells, taking out the chicken embryos, homogenizing the chicken embryos with a tissue homogenizer at 4°C, pouring them into a sterilized sealed box, Put it in a freezer at -30°C for 72 hours, and after thawing, perform cell ultrasonic disruption in an environment of 4°C, add an equal amount of water for injection, and place it in a freezer at 4°C for use.
[0064] (3) Enzymatic hydrolysis and centrifugation: Take the refrigerated solution after cell crushing, heat it in a water bath to 50°C, adj...
Embodiment 2
[0067] (1) Hatching chicken embryos: Take SPF grade fertilized eggs and put them into the incubator for incubation. The incubation temperature is controlled at 37.5°C, the relative humidity is 60%, and the eggs are automatically turned every 2 hours. After hatching to 14 days, take out the fertilized eggs , put it in a freezer at -20°C, stop the incubation and save it for later use.
[0068] (2) Homogenization and cell crushing: surface sterilize the hatched eggs, remove the eggshells and take out the chicken embryos, homogenize the chicken embryos with a tissue homogenizer at 8°C, and pour them into a sterilized sealed box. Put it in a freezer at -20°C for 80 hours, and after thawing, perform cell ultrasonic disruption in an environment of 4°C, add an equal amount of water for injection, and place it in a freezer at 4°C for use.
[0069] (3) Enzymatic hydrolysis and centrifugation: Take the refrigerated liquid after cell crushing, heat it in a water bath to 55°C, adjust the p...
Embodiment 3
[0072] (1) Hatching chicken embryos: Take SPF grade fertilized eggs and put them in the incubator for incubation. The incubation temperature is controlled at 37.5°C and the relative humidity is 60%. The eggs are automatically turned every 2 hours. After hatching to 19 days, take out the fertilized eggs , put it in a freezer at -20°C, stop the incubation and save it for later use.
[0073] (2) Homogenization and cell crushing: surface disinfection of hatched eggs, removal of egg shells, taking out chicken embryos, homogenizing chicken embryos with a tissue homogenizer at 0°C, pouring them into a sterilized sealed box, Put it in a freezer at -40°C for 60 hours, and after thawing, perform cell ultrasonic disruption in an environment of 0°C, add an equal amount of water for injection, and place it in a freezer at 0°C for use.
[0074] (3) Enzymatic hydrolysis and centrifugation: take the frozen frozen solution after cell crushing, heat it in a water bath to 45°C, adjust the pH to ...
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