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Ultrasound contrast agent based on pentaerythritol esters, and preparation method and application thereof

An ultrasonic contrast agent, pentaerythritol ester technology, applied in the field of medicine, can solve the problems of high cost and the like, and achieve the effects of low cost, good degradability, and good contrast enhancement effect

Active Publication Date: 2016-05-04
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

And the price of SonoVue is $110 / bottle, which is expensive

Method used

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  • Ultrasound contrast agent based on pentaerythritol esters, and preparation method and application thereof
  • Ultrasound contrast agent based on pentaerythritol esters, and preparation method and application thereof
  • Ultrasound contrast agent based on pentaerythritol esters, and preparation method and application thereof

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Embodiment 1

[0048] Embodiment 1: Preparation of ultrasound contrast agent

[0049] This embodiment provides a pentaerythritol ester-based coated microbubble ultrasound contrast agent, which specifically includes the following steps:

[0050] Step (1): Take 1.77g of pentaerythritol monostearate, 1g of polyethylene glycol monostearate and 1.50g of NaCl, grind them thoroughly in a mortar, disperse them in 50mL of PBS, stir them evenly to obtain a suspension solution; the suspension was placed in a sterilizing pot at 120° C. and sterilized under a pressure of 0.2 MPa for 12 minutes, then continuously stirred, and cooled to room temperature to obtain a coating solution;

[0051] Step (2): Pass into perfluoropropane (C 3 f 8 ) gas, use the “1 / 2” probe of the Sonicator4000 ultrasonic breaker to continuously ultrasonicate for 3 minutes at the maximum amplitude to obtain a uniform milky white suspension, collect it in a 250mL separatory funnel and let it stand for 35 minutes. Bubbles of differen...

Embodiment 2

[0054] Embodiment 2: the preparation of ultrasound contrast agent

[0055] This embodiment provides a pentaerythritol ester-based coated microbubble ultrasound contrast agent, which specifically includes the following steps:

[0056] Step (1): In parts by mass, 1.77g of pentaerythritol monostearate, 1g of polyethylene glycol monostearate and 1.5g of NaCl are added in 20mL of chloroform to dissolve completely, and rotary evaporation is to remove chloroform, then Add 50mL of PBS buffer solution, and ultrasonically disperse in a water bath to obtain a coating solution;

[0057] Step (2): Pass SF into the coating solution 6 For gas, use the "1 / 2" probe of Sonicator4000 ultrasonic breaker to continuously ultrasonicate for 4min at the maximum amplitude to obtain a uniform milky white suspension, which is collected in a 250mL separatory funnel and left to stand for 30min. Bubbles of different sizes are due to their own The buoyancy effect is divided into three distinct layers, and ...

Embodiment 3

[0060] Embodiment 3: the preparation of ultrasound contrast agent

[0061] This embodiment provides a pentaerythritol ester-based coated microbubble ultrasound contrast agent, which specifically includes the following steps:

[0062] Step (1): Take 0.855g of pentaerythritol monopalmitate, 601.5g of Tween surfactant, 1g of macromolecular polymer polyethylene glycol difatty acid ester, and 1.73g of phospholipid compound lecithin, add PBS50mL, and mix well , preparing the coating solution;

[0063] Step (2): Pass SF into the coating solution 6 For gas, use the "1 / 2" probe of Sonicator4000 ultrasonic breaker to continuously ultrasonicate for 4 minutes at the maximum amplitude to obtain a milky white suspension; collect it in a 250mL separatory funnel and let it stand for 30 minutes. Divided into three distinct layers, collecting microbubbles in the middle layer and rinsing repeatedly with PBS buffer solution until the solution in the lower layer is clear to obtain an ultrasound ...

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Abstract

The invention relates to the field of medicine and specifically discloses an ultrasound contrast agent based on pentaerythritol esters, and a preparation method and application thereof. An encapsulating material of the ultrasonic contrast agent at least includes one pentaerythritol ester and may further include a surfactant, a high-molecular polymer, a phosphatide compound and other components; and the encapsulating material is filled with gas or liquid to form the ultrasound contrast agent with a micro-bubble structure. An acoustic-vibration cavitation method is employed to prepare the ultrasound contrast agent, and the method is simple and controllable; and the prepared ultrasound contrast agent has good biocompatibility, good acoustic responsiveness, long development action time, low cost, suitability for industrial production and the like, can be used as a medicine and genetic vector and has wide clinical application prospects.

Description

technical field [0001] The invention relates to the field of medicine, and specifically discloses a pentaerythritol ester-based ultrasonic contrast agent as well as a preparation method and application thereof. Background technique [0002] Ultrasound imaging technology refers to the use of differences in the acoustic properties of human tissues and organs to reflect the physiological and pathological information inside the tissue by using the difference in the strength of the reflection and scattering signals of ultrasound in different tissues, so as to achieve the purpose of diagnosis. Compared with other imaging methods, ultrasonography is painless, non-radioactive, and does not require special environmental conditions. The information provided is accurate, rich, and intuitive, and is widely welcomed by patients and doctors. Ultrasound medicine has developed very rapidly, and it has become an indispensable routine inspection method in clinical practice in just a few decad...

Claims

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Application Information

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IPC IPC(8): A61K49/22
CPCA61K49/221A61K49/223
Inventor 戴志飞梁晓龙刘仁发高闯
Owner PEKING UNIV
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