Ultrasound contrast agent based on pentaerythritol esters, and preparation method and application thereof

An ultrasonic contrast agent, pentaerythritol ester technology, applied in the field of medicine, can solve the problems of high cost and the like, and achieve the effects of low cost, good degradability, and good contrast enhancement effect

Active Publication Date: 2016-05-04
PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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  • Ultrasound contrast agent based on pentaerythritol esters, and preparation method and application thereof
  • Ultrasound contrast agent based on pentaerythritol esters, and preparation method and application thereof
  • Ultrasound contrast agent based on pentaerythritol esters, and preparation method and application thereof

Examples

Experimental program
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Example Embodiment

[0048] Example 1: Preparation of ultrasound contrast agent

[0049] This embodiment provides a pentaerythritol ester-based coated microbubble ultrasound contrast agent, which specifically includes the following steps:

[0050] Step (1): Take 1.77g of pentaerythritol monostearate, 1g of polyethylene glycol monostearate and 1.50g of NaCl, place them in a mortar and grind them thoroughly, disperse them in 50mL PBS, magnetically stir and mix to obtain a suspension The suspension is placed in a 120°C sterilization pot and sterilized under a pressure of 0.2MPa for 12 minutes, and then continuously stirred and cooled to room temperature to obtain a coating solution;

[0051] Step (2): pass perfluoropropane (C) into the coating solution 3 F 8 ) Gas, use the "1 / 2" probe of the Sonicator4000 ultrasonic breaker to continuously sonicate for 3 minutes at the maximum amplitude to obtain a uniform milky white suspension, which is collected in a 250mL separatory funnel and allowed to stand for 35 mi...

Example Embodiment

[0054] Example 2: Preparation of ultrasound contrast agent

[0055] This embodiment provides a pentaerythritol ester-based coated microbubble ultrasound contrast agent, which specifically includes the following steps:

[0056] Step (1): In parts by mass, add 1.77 g of pentaerythritol monostearate, 1 g of polyethylene glycol monostearate and 1.5 g of NaCl to 20 mL of chloroform to completely dissolve, rotary evaporation to remove the chloroform, and then Add 50mL PBS buffer, and ultrasonically disperse in a water bath to obtain a coating solution;

[0057] Step (2): pass SF into the coating solution 6 Use the “1 / 2” probe of the Sonicator4000 ultrasonic breaker to sonicate continuously for 4 minutes at the maximum amplitude to obtain a uniform milky white suspension. After collecting it in a 250mL separatory funnel and letting it stand for 30 minutes, bubbles of different sizes are due to their own The buoyancy effect is divided into three obvious layers. The microbubbles in the middl...

Example Embodiment

[0060] Example 3: Preparation of ultrasound contrast agent

[0061] This embodiment provides a pentaerythritol ester-based coated microbubble ultrasound contrast agent, which specifically includes the following steps:

[0062] Step (1): Take 0.855g of pentaerythritol monopalmitate, 601.5g of surfactant Tween, 1g of macromolecular polymer polyethylene glycol difatty acid ester, and 1.73g of phospholipid compound lecithin, add 50mL of PBS, and mix well , Preparation of coating solution;

[0063] Step (2): pass SF into the coating solution 6 The gas is treated with the "1 / 2" probe of the Sonicator4000 ultrasonic breaker under the maximum amplitude of continuous ultrasonic treatment for 4 minutes to obtain a milky white suspension; after collecting it in a 250mL separatory funnel and standing for 30 minutes, bubbles of different sizes are due to their own buoyancy Divide into three obvious layers, collect microbubbles in the middle layer and rinse with PBS buffer solution repeatedly unt...

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Abstract

The invention relates to the field of medicine and specifically discloses an ultrasound contrast agent based on pentaerythritol esters, and a preparation method and application thereof. An encapsulating material of the ultrasonic contrast agent at least includes one pentaerythritol ester and may further include a surfactant, a high-molecular polymer, a phosphatide compound and other components; and the encapsulating material is filled with gas or liquid to form the ultrasound contrast agent with a micro-bubble structure. An acoustic-vibration cavitation method is employed to prepare the ultrasound contrast agent, and the method is simple and controllable; and the prepared ultrasound contrast agent has good biocompatibility, good acoustic responsiveness, long development action time, low cost, suitability for industrial production and the like, can be used as a medicine and genetic vector and has wide clinical application prospects.

Description

technical field [0001] The invention relates to the field of medicine, and specifically discloses a pentaerythritol ester-based ultrasonic contrast agent as well as a preparation method and application thereof. Background technique [0002] Ultrasound imaging technology refers to the use of differences in the acoustic properties of human tissues and organs to reflect the physiological and pathological information inside the tissue by using the difference in the strength of the reflection and scattering signals of ultrasound in different tissues, so as to achieve the purpose of diagnosis. Compared with other imaging methods, ultrasonography is painless, non-radioactive, and does not require special environmental conditions. The information provided is accurate, rich, and intuitive, and is widely welcomed by patients and doctors. Ultrasound medicine has developed very rapidly, and it has become an indispensable routine inspection method in clinical practice in just a few decad...

Claims

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Application Information

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IPC IPC(8): A61K49/22
CPCA61K49/221A61K49/223
Inventor 戴志飞梁晓龙刘仁发高闯
Owner PEKING UNIV
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