Ultrasound contrast agent based on pentaerythritol esters, and preparation method and application thereof
An ultrasonic contrast agent, pentaerythritol ester technology, applied in the field of medicine, can solve the problems of high cost and the like, and achieve the effects of low cost, good degradability, and good contrast enhancement effect
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[0048] Example 1: Preparation of ultrasound contrast agent
[0049] This embodiment provides a pentaerythritol ester-based coated microbubble ultrasound contrast agent, which specifically includes the following steps:
[0050] Step (1): Take 1.77g of pentaerythritol monostearate, 1g of polyethylene glycol monostearate and 1.50g of NaCl, place them in a mortar and grind them thoroughly, disperse them in 50mL PBS, magnetically stir and mix to obtain a suspension The suspension is placed in a 120°C sterilization pot and sterilized under a pressure of 0.2MPa for 12 minutes, and then continuously stirred and cooled to room temperature to obtain a coating solution;
[0051] Step (2): pass perfluoropropane (C) into the coating solution 3 F 8 ) Gas, use the "1 / 2" probe of the Sonicator4000 ultrasonic breaker to continuously sonicate for 3 minutes at the maximum amplitude to obtain a uniform milky white suspension, which is collected in a 250mL separatory funnel and allowed to stand for 35 mi...
Example Embodiment
[0054] Example 2: Preparation of ultrasound contrast agent
[0055] This embodiment provides a pentaerythritol ester-based coated microbubble ultrasound contrast agent, which specifically includes the following steps:
[0056] Step (1): In parts by mass, add 1.77 g of pentaerythritol monostearate, 1 g of polyethylene glycol monostearate and 1.5 g of NaCl to 20 mL of chloroform to completely dissolve, rotary evaporation to remove the chloroform, and then Add 50mL PBS buffer, and ultrasonically disperse in a water bath to obtain a coating solution;
[0057] Step (2): pass SF into the coating solution 6 Use the “1 / 2” probe of the Sonicator4000 ultrasonic breaker to sonicate continuously for 4 minutes at the maximum amplitude to obtain a uniform milky white suspension. After collecting it in a 250mL separatory funnel and letting it stand for 30 minutes, bubbles of different sizes are due to their own The buoyancy effect is divided into three obvious layers. The microbubbles in the middl...
Example Embodiment
[0060] Example 3: Preparation of ultrasound contrast agent
[0061] This embodiment provides a pentaerythritol ester-based coated microbubble ultrasound contrast agent, which specifically includes the following steps:
[0062] Step (1): Take 0.855g of pentaerythritol monopalmitate, 601.5g of surfactant Tween, 1g of macromolecular polymer polyethylene glycol difatty acid ester, and 1.73g of phospholipid compound lecithin, add 50mL of PBS, and mix well , Preparation of coating solution;
[0063] Step (2): pass SF into the coating solution 6 The gas is treated with the "1 / 2" probe of the Sonicator4000 ultrasonic breaker under the maximum amplitude of continuous ultrasonic treatment for 4 minutes to obtain a milky white suspension; after collecting it in a 250mL separatory funnel and standing for 30 minutes, bubbles of different sizes are due to their own buoyancy Divide into three obvious layers, collect microbubbles in the middle layer and rinse with PBS buffer solution repeatedly unt...
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