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A kind of jatrorrhizine derived from Phellodendron cortex processed product and its separation preparation method and application

A technology of jatrorrhizine and processed products, applied in the directions of drug combination, organic chemistry, allergic diseases, etc., can solve problems such as differences in pharmacological effects and changes in drug properties, and achieve the effects of simple operation, good phagocytosis, and high efficiency.

Active Publication Date: 2017-05-17
LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the influence of temperature, the chemical composition of Cortex Phellodendri will change to a certain extent during the processing process, and its medicinal properties will also change due to the addition of different excipients, which will eventually lead to differences in pharmacological effects.

Method used

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  • A kind of jatrorrhizine derived from Phellodendron cortex processed product and its separation preparation method and application
  • A kind of jatrorrhizine derived from Phellodendron cortex processed product and its separation preparation method and application
  • A kind of jatrorrhizine derived from Phellodendron cortex processed product and its separation preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Instruments and materials.

[0022] 1.1 Instruments: semi-preparative liquid chromatograph (Shimadzu LC-10AT); METTLER AE240 1 / 100,000 analytical balance (Switzerland METTLER); FA1004B electronic balance (Shanghai Precision Scientific Instrument Co., Ltd.); YP5102 electronic balance ( Shanghai Guangzheng Medical Instrument Co., Ltd.); KQ-250DB CNC ultrasonic cleaner (Kunshan Ultrasonic Instrument Co., Ltd.); nitrogen blowing instrument (Tianjin Hengao Technology Development Co., Ltd.); 101 electric blast drying oven (Beijing Yong Guangming Medical Instrument Factory); CHRIST Vacuum Freeze Dryer (Germany); CAMAG Linomat-5 Semi-automatic Spotting Apparatus (Switzerland).

[0023] 1.2 Reagents: Phellodendron Phellodendri was purchased from Sichuan Medicinal Materials Company; jatrorrhizine hydrochloride reference substance was purchased from Sichuan Weikeqi Biotechnology Co., Ltd. (batch number 130218); Determination, purity > 98%; thin-layer chromatography silica gel ...

Embodiment 2

[0059] This embodiment provides a method for the separation and preparation of jatorrhizine derived from cortex cortex processed products, including the following steps.

[0060] Step 1. Simulate processing of the jatrorrhizine reference substance, which is an index component in Cortex Phellodendri, accurately weigh 5 mg of jatrorrhizine hydrochloride reference substance, put it in an evaporating dish, bake it at 160°C for 15 minutes, take it out and let it cool, and add chromatographic grade Methanol 5mL was filtered through a microporous membrane (0.45 μm) to obtain the baked jatrorrhizine reference solution for the preparation of new components.

[0061] Step 2. Separation and preparation of new components of baked products of jatrorrhizine monomer.

[0062] Preparative liquid chromatography conditions: mobile phase A is water, of which 100ml water contains 0.4ml diethylamine, B is methanol; mobile phase flow rate: the mobile phase ratio of A and B is (70:30); 18 The silic...

Embodiment 3

[0065] This embodiment provides a method for the separation and preparation of jatorrhizine derived from cortex cortex processed products, including the following steps.

[0066] Step 1. Simulate the preparation of the jatrorrhizine reference substance, an index component in Cortex Phellodendri, accurately weigh 5 mg of jatrorrhizine hydrochloride reference substance, put it in an evaporating dish, bake it at 180°C for 25 minutes, take it out and let it cool, and add chromatographic grade Methanol 5mL was filtered through a microporous membrane (0.45 μm) to obtain the baked jatrorrhizine reference solution for the preparation of new components.

[0067] Step 2. Separation and preparation of new components of baked products of jatrorrhizine monomer.

[0068] Preparative liquid chromatography conditions: mobile phase A is water, of which 100ml water contains 0.4ml diethylamine, B is methanol; mobile phase flow rate: the mobile phase ratio of A and B is (90:10); 18 The silica ge...

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Abstract

The invention provides medicine root berberrubine derived from an amur cork-tree processed product and a separation preparation method and application thereof. The method includes the steps that 1, jatrorrhizine hydrochloride reference substances are precisely weighed, placed in an evaporation dish to be baked, taken out and cooled, chromatographic grade methyl alcohol is added, millipore filter filtering is conducted, and a baked jatrorrhizine reference substance solution is obtained; 2, the baked jatrorrhizine reference substance solution is separated through a preparative liquid phase, eluant of a target chromatographic peak is collected and purified through a sephadex column, the purified eluant is subjected to nitrogen blowing and freeze drying, and pink powder, namely, medicine root berberrubine crystals prepared through separation are obtained. The prepared medicine root berberrubine is a new compound found in amur cork-tree processed decoction pieces, the separation preparation method has the advantages of being high in efficiency, stable in process, easy and convenient to operate and low in cost, high-purity separation preparation of a large number of medicine root berberrubine monomers can be achieved, and the medicine root berberrubine compound has good macrophage phagocytosis and high immunization.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine, in particular to a jatorrhizine derived from cortex cortex processed products and its separation and preparation method and application. Background technique [0002] Phellodendron cortex was first recorded in "Shen Nong's Materia Medica", and it is a plant of Rutaceae. Phellodendron chinenseSchneid. or Phellodendron Phellodendron The dry bark of amurense Rupr. has a medicinal history of more than 2,000 years. It is a traditional Chinese medicine for clearing away heat and purging fire. Physicians of all dynasties attached great importance to the processing of Cortex Phellodendri in clinical application. Its processed products were first seen in Jin Dynasty, Ge Hong's "Elbow Reserve Emergency Prescription". The traditional processing methods of Phellodendron include 16 processing methods such as net processing, cutting, honey processing, wine processing, salt processing, c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D455/03A61K31/4375A61P37/02
CPCC07D455/03
Inventor 张凡刘蓬蓬贾天柱徐珊高慧史辑鞠成国林桂梅单国顺
Owner LIAONING UNIV OF TRADITIONAL CHINESE MEDICINE
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