Soil ecological restoration agent for solving old ginseng land continuous cropping obstacle and preparation method thereof

A technology for ecological restoration and continuous cropping obstacles, which is applied in the restoration of polluted soil, botanical equipment and methods, biocides, etc. , the effect of promoting growth

Active Publication Date: 2016-05-11
江瀚生物科技(上海)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] At this stage, methods for improving ginseng waste soil have been published. Among them, CN104509245A discloses a soil improvement method for waste ginseng land, which mainly includes the steps of soil disinfection, soil remediation, and phytorem

Method used

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  • Soil ecological restoration agent for solving old ginseng land continuous cropping obstacle and preparation method thereof

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preparation example Construction

[0029] The invention provides a preparation method of a soil ecological restoration agent for solving the obstacles of continuous cropping of old ginseng, which is characterized in that it comprises:

[0030] Step 1: According to 20-40 parts of Bacillus licheniformis, 10-20 parts of Candida utilis, 10-30 parts of Lactobacillus plantarum, 10-20 parts of Trichoderma viride, 5-10 parts of Rhodopseudomonas palustris The weight ratio is mixed to obtain the composite bacteria;

[0031] Step 2: Add the composite bacteria to the composite medium according to the weight ratio of 1:80-120, stir evenly, and cultivate it at 28-32°C until the pH is less than 4.00, and then culture it in a closed manner for 12-15 days to obtain a liquid The described soil ecological restoration agent of form.

[0032] Preferably, where:

[0033] The Bacillus licheniformis (Bacilus subtilis) is a bacterial species whose preservation number is ACCC10146;

[0034] The Candida utilis yeast (Candidautilis) is...

Embodiment 1

[0053] A preparation method of a soil ecological restoration agent for solving continuous cropping obstacles of old ginseng, comprising:

[0054] Step 1: Cultivation of the strains: insert the Bacillus licheniformis strains purchased from the China Agricultural Microorganism Strain Collection and Management Center into an Erlenmeyer flask of nutrient broth liquid medium, and cultivate them with shaking at 220 rpm at 38°C for 24 hours, and wait until Take out after the medium is turbid; put the strain of Candida utilis into the Erlenmeyer flask of potato liquid medium, shake and culture at 30°C and 220rpm for 48 hours, take it out after the medium is turbid; put the strain of Lactobacillus plantarum into MRS In the Erlenmeyer flask of liquid medium, culture at 37°C for 48 hours, take out after the medium is turbid; put the Trichoderma viride strains into the Erlenmeyer flask of potato liquid medium, culture at 28°C, 220rpm shaking for 72 hours, Take out after the culture medium...

Embodiment 2

[0058] A kind of preparation method of the soil ecological remediation agent that solves the continuous cropping obstacle of old ginseng, continues to prepare on the basis of embodiment 1, comprises:

[0059] Step 3: After mixing corn stalks, cow dung, molasses and the soil ecological restoration agent obtained in Example 1 in the mixer according to the weight ratio of 1:4:0.15:0.15, put them into a container with an exhaust valve. sealed in an aluminum foil bag, and completely anaerobically fermented at 28°C for two weeks to obtain the soil ecological restoration agent in solid form;

[0060] Wherein, after the corn is harvested, the stalks are flattened in the field, dried, collected, transported back to the production plant for temporary storage in the greenhouse, kept dry in the greenhouse, the crushed length of the straw is 3 mm, and the straw should be kept dry to obtain the corn stalk;

[0061] Collect fresh cow manure and store it in the dung storage tank for later use...

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Abstract

The invention provides a preparation method of a soil ecological restoration agent for solving old ginseng land continuous cropping obstacle. The preparation method includes the steps: (1) mixing 20-40 parts of bacillus licheniformis, 10-20 parts of candida utilis, 10-30 parts of lactobacillus plantarum, 10-20 parts of trichoderma viride and 5-10 parts of rhodopseudomonas palustris according to the weight ratio to obtain compound bacteria; (2) adding the compound bacteria to a compound culture medium according to the weight ratio of 1:(80-120), stirring evenly, then culturing under a condition of the temperature of 28-32 DEG C until the pH is less than 4.00, then carrying out closed culture for 12-15 days, and thus obtaining the soil ecological restoration agent in a liquid form; and furthermore, making the liquid form into a solid form. Through the production of the microbial inoculant, starting from restoration of a soil ecological system, and with use of the multi-strain combined technology method, beneficial bacteria in soil and beneficial nutritional factors for promoting the growth of ginseng are increased, and harmful bacteria generated by continuous cropping are inhibited.

Description

technical field [0001] The invention relates to the field of soil improvement, in particular to a soil ecological restoration agent, in particular to a soil ecological restoration agent which solves the continuous cropping obstacle of old ginseng and a preparation method thereof. Background technique [0002] Along with the raising of living economy level, people are constantly increasing for the demand of ginseng as top grade nourishing. At present, due to the continuous cropping obstacles in ginseng planting, the continuous development of new ginseng planting areas has become the main way to solve the large supply of ginseng. Among them, as an important ginseng producing area in Northeast my country, a large number of virgin woodlands are cut down every year to be used as ginseng planting areas, thus causing great damage to the ecological environment. In fact, the formation of continuous cropping obstacles is mainly due to the accumulation of harmful microorganisms in the...

Claims

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Application Information

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IPC IPC(8): C09K17/14A01N63/02A01N63/04A01P21/00B09C1/10C09K101/00
CPCA01N63/10A01N63/30B09C1/105B09C2101/00C09K17/14C09K2101/00A01N2300/00
Inventor 江瀚
Owner 江瀚生物科技(上海)有限公司
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