Enzymolysis device, enzymolysis system comprising same, and method for enzymolysis of raw materials containing cellulose

A cellulose and enzymatic hydrolysis technology, applied in biochemical equipment and methods, enzymology/microbiology equipment, biochemical cleaning equipment, etc., can solve the problems of low enzyme utilization efficiency, low sugar production efficiency, poor enzymatic hydrolysis effect, etc. question

Active Publication Date: 2016-05-11
COFCO NUTRITION & HEALTH RES INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to overcome the defects of low enzyme utilization efficiency, poor enzymolysis effect and low sugar production efficiency in the enzymolysis method of cellulose-containing raw materials in the prior art, and to provide an enzymolysis device and the An enzymolysis system of an enzymolysis device and a method for enzymolysis of cellulose-containing raw materials, the method of enzymatic hydrolysis of cellulose-containing raw materials by using the enzymolysis system including the enzymolysis device can effectively improve the utilization rate of enzymes and improve the efficiency of enzymatic hydrolysis. Efficiency and Monosaccharide Yield

Method used

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  • Enzymolysis device, enzymolysis system comprising same, and method for enzymolysis of raw materials containing cellulose
  • Enzymolysis device, enzymolysis system comprising same, and method for enzymolysis of raw materials containing cellulose
  • Enzymolysis device, enzymolysis system comprising same, and method for enzymolysis of raw materials containing cellulose

Examples

Experimental program
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Effect test

Embodiment 1

[0089] This example is used to illustrate the enzymatic hydrolysis method of the cellulose-containing raw material of the present invention.

[0090] (1) Pretreatment of cellulose-containing raw materials

[0091] Cut 1,333 kg of impurity-free corn stalks (with a water content of 10% by weight) into small sections not exceeding 1.2 cm × 0.5 cm × 1.0 cm, maintain a pressure of 1.0 MPa at 180 ° C for 5 minutes, and then release the pressure to complete steam explosion . A total of 4000 kg of steam explosion waste (moisture content 70% by weight) was obtained.

[0092] Determination of the total weight of cellulose and the total weight of hemicellulose in the resulting solid steam explosion product:

[0093] Take 10 grams of the above-mentioned steam explosion product and dry it at 45° C. to a constant weight of 5 grams, weigh 300.0 mg of the dried steam explosion product, and place it in a 100-milliliter dry Erlenmeyer flask weighing 80 grams. Add 3.00 ml of sulfuric acid sol...

Embodiment 2

[0104] This example is used to illustrate the enzymatic hydrolysis method of the cellulose-containing raw material of the present invention.

[0105] Carry out the enzymolysis of cellulose-containing raw material according to the method for embodiment 1, difference is:

[0106] In the enzymolysis step of step (2), the temperature in the main enzymolysis device 2 is 40°C, the addition of the steam explosion product is 320 kg / hour, and the pH value is adjusted to 6. In terms of per gram of cellulose, the fiber The consumption of prime enzyme is 20 enzyme activity units, and in described continuous enzymolysis stage, the addition of enzyme is 70% of the total consumption of enzymolysis process enzyme (the total enzyme consumption of main enzymolysis stage is 5.88 * 10 6enzyme activity unit), therefore, the addition of the cellulase that continuously adds in the main enzymolysis device 2 is 4.7 * 10 5 Enzyme activity unit / hour, the added amount of hemicellulase is 10% of the tota...

Embodiment 3

[0109] This example is used to illustrate the enzymatic hydrolysis method of the cellulose-containing raw material of the present invention.

[0110] Carry out the enzymolysis of cellulose-containing material according to the method of Example 1, the difference is that in the enzymolysis step of step (2), in the main enzymolysis stage, all cellulase and all hemicellulase are extracted from The enzyme inlet 27 at the top of the tank 21 is continuously introduced into the tank 21 . From the enzymolysis product of the main enzymolysis stage, the monosaccharide content is 82g / L, of which the glucose content is 45g / L, the xylose content is 27g / L, and the total content of arabinose, galactose and mannose is 10g / L; Based on the final degradation to glucose content, the soluble glucan content is 43g / L; based on the final degradation to xylose content, the soluble xylan content is 28g / L; the viscosity is 300cp, and the average particle size is 30μm.

[0111] In the intermittent enzyma...

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Abstract

The invention discloses an enzymolysis device. The enzymolysis device comprises a tank body (21), a jacket arranged on the periphery of the tank body (21) in a covering mode and provided with an inlet and an outlet, a stirring shaft (22) arranged in the tank body (21), and partition boards (23), wherein the tank body (21) is divided into a plurality of enzymolysis spaces in the height direction by the partition boards (23), through holes allowing every two adjacent enzymolysis spaces obtained through division of the partition boards (23) to be communicated are formed in the partition boards (23), and a stirrer is arranged on the portion, where the corresponding stirring shaft (22) is arranged, in each enzymolysis space obtained through division of the partition boards (23) in the length direction. The enzymolysis device further comprises a cellulosic material inlet (26), an enzyme inlet (27) and a material outlet (28) which are communicated with the tank body (21). The invention further discloses an enzymolysis system and a method for enzymolysis of raw materials containing cellulose by means of the enzymolysis system. By the adoption of the method, the utilization rate of enzyme can be effectively increased, and enzymolysis efficiency and monosaccharide yield can be increased.

Description

technical field [0001] The invention relates to an enzymolysis device, an enzymolysis system comprising the enzymolysis device and an enzymolysis method for cellulose-containing raw materials. Background technique [0002] The enzymatic hydrolysis process of cellulose-containing raw materials in the prior art usually adopts a stirring batch operation. In the presence of water, the cellulose-containing raw material and enzyme are added to the enzymolysis reactor at the same time, and the mixed reaction is adjusted to an appropriate pH value. , to maintain the reaction conditions for enzymatic hydrolysis to the end. Before enzymatic hydrolysis, the cellulose-containing raw material needs to be pretreated to expose the cellulose in the cellulose-containing raw material, so as to increase the contact area between the enzyme and the cellulose. [0003] The pretreatment of cellulose-containing raw materials by the method of the prior art will bring a series of problems to the sub...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/02C12M1/00C12P19/14
Inventor 武国庆林鑫熊强林海龙李凡沈乃东许克家高尚袁敬伟李春玲刘文信
Owner COFCO NUTRITION & HEALTH RES INST
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