Unlock instant, AI-driven research and patent intelligence for your innovation.
RT-rpa detection kit for rapid detection of highly pathogenic porcine reproductive and respiratory syndrome virus and its use
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A respiratory syndrome, highly pathogenic technology, applied in the directions of microorganism-based methods, microbial determination/examination, biochemical equipment and methods, etc., to achieve the effect of good specificity, increased specificity, and simple method
Active Publication Date: 2018-12-07
LANZHOU INST OF VETERINARY SCI CHINESE ACAD OF AGRI SCI
View PDF4 Cites 2 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
At present, there is no RT-RPA test established at home and abroad to detect HP-PRRSV
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment 1
[0037] The establishment of the RT-RPA detection kit and detection method of embodiment 1 rapid detection HP-PRRSV
[0038] 1. Design and synthesis of primers and probe sequences
[0039] Studies have shown that compared with C-PRRSV, H-PRRSV lacks about 30 amino acids in the NSP2 region, so the present invention selects highly pathogenic porcine reproductive and respiratory syndrome virus NSP2 deletion region to design specific primers and probes, and The homologous sequences of NSP2 genes from EF112445, EF517962, EF635006, EF641008, EU109503, EU144079, EU187484, EU200961, EU880431 and EU880435 in GenBank were compared in order to detect as many HP-PRRSV as possible. Both probes and probes were synthesized by Sangon Biotech (Shanghai, China).
[0041] All strains used in this study are preserved by our laboratory: HP-PRRSV / SD0907 (GenBank: KF562320.1), HP-PRRSV / JS0912 (GenBank: KF562318.1), classical PRRSV strain CH-1R (GenBank: EU807840...
Embodiment 2
[0064] The purposes of the RT-RPA detection kit of embodiment 2HP-PRRSV in field detection
[0065] 1. Sample
[0066] Sixty-eight field tissue samples were collected from eight pig farms suspected to have HP-PRRS in Shandong Province. Twelve serum samples were collected from healthy pigs. Viral genome extraction is the same as in Example 1.
[0067] 2. Detection method
[0068] (1) real-time RT-PRA method
[0069] The experimental system was as follows: 13.75 μL hydrolysis buffer, 1.05 μL upstream primer (10 μM), 1.05 μL downstream primer (10 μM), 0.075 μL RPA exo probe (10 μM), 2 μL RNA template, 4.825 μL ddHO and 1.25 μL Magnesium acetate (280 mM).
[0070] The sequences of the primers and probes are as follows:
[0071] Upstream primer: 5'-AGCTGATGACACCTTTGAGTGGGTCGGCACCAGTT-3' (shown in SEQ ID NO.1);
[0072] Downstream primer: 5'-CGTCTGTGAGGACGCAGACAAATCCAGAGGCTCAT-3' (shown in SEQ ID NO.2);
[0073] Probe: 5'-GTCGGCACCAGTTCCTGCACCGCGTAGAAC
[0074] -(FAM-dT)-(T...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
technical field [0001] The invention relates to a kit for detecting highly pathogenic porcine reproductive and respiratory syndrome virus and its application, in particular to an RT-RPA detection kit for rapidly detecting highly pathogenic porcine reproductive and respiratory syndrome virus and its application. purposes, the invention belongs to the field of preventive veterinary medicine inspection. Background technique [0002] In the laboratories of developing countries, due to the limitation of basic equipment and technology required for PCR implementation, most developing countries still focus on using traditional test methods, such as serological methods, microscopy techniques, or culturing and identifying infectivity and non-communicable diseases. In most cases, despite the lack of experimental equipment to use these methods, and the lack of routine practice of comprehensive management of these diseases. As a result, these countries have long been plagued by disease...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to View More 
Login to View More 
