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Non-target quick screening and detecting method for PDE-5 inhibitor banned drugs in health foods

A PDE-5, health food technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve problems such as incomplete detection and analysis, and achieve low detection limit, small matrix effect, and good linearity

Inactive Publication Date: 2016-05-11
INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, existing literature studies have found that more than 80 kinds of PDE-5 inhibitors are added to health food, and the conventional chromatography-mass spectrometry method cannot effectively detect and analyze these prohibited drug ingredients.

Method used

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  • Non-target quick screening and detecting method for PDE-5 inhibitor banned drugs in health foods
  • Non-target quick screening and detecting method for PDE-5 inhibitor banned drugs in health foods
  • Non-target quick screening and detecting method for PDE-5 inhibitor banned drugs in health foods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] 1. Preparation of standard solutions of 13 kinds of PDE-5 inhibitor drugs: Weigh 2.0mg of each standard PDE-5 inhibitor drug, put it in a 10mL volumetric flask, dilute to the mark with methanol, and prepare a standard stock solution of 200μg / mL . Dilute with methanol to prepare a 10 μg / mL mixed standard solution according to the needs of the experiment, dilute to prepare a series of standard working solutions, and store at 4 °C.

[0026] 2. QuEChERS technology and ultrasonic-assisted extraction pretreatment method: use the response surface experimental design method, use the Box-Behnken experimental design scheme, the four factors are, ACN:H2O ratio (, %v / v), MgSO4&NaCl dosage (, mg ), adsorbent C18 (, mg), ultrasonic time (, min), and the quadratic regression equation model predicts and evaluates the recovery rate of 13 PDE-5 inhibitors, so as to optimize the four sample pretreatment extraction factors. The table header of Box-Behnken experimental design is as follows...

Embodiment 2

[0049] Take 1mL of certain tonic wine, add 15mL of acetonitrile and water mixed solution (the volume ratio of acetonitrile and water is 7:3), mix well, add 1g of magnesium sulfate and 0.25g of sodium chloride, fully vortex on a micro mixer for 1min , sonicate for 10 minutes, centrifuge at 6000r / min for 5 minutes, transfer the supernatant to a 25mL graduated tube, and pass 1mL of the solution through a 0.45μm organic microporous nylon filter membrane for IDA-MS detection and analysis. See attached table 5 for the screening data table of PDE-5 inhibitor prohibited drugs. According to the high-resolution mass spectrometry data information, it can quickly screen and analyze the prohibited drugs in health tonic wine (attached figure 4). A sample of tonic wine contains demethylated Sildenafil is a prohibited drug ingredient.

[0050] Table 5 is the Masterviews database of 86 PDE-5 inhibitor banned drugs

[0051]

[0052]

[0053]

[0054]

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PUM

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Abstract

The invention belongs to the technical field of detection of unknown components in foods and particularly discloses a non-target quick screening and detecting method for illegally-added male aphrodisiac PDE-5 inhibitor western drugs in health foods. The combination of QuEChERS (quick, easy, cheap, effective, rugged, and safe) technology with ultrasound-assisted extraction technology is used for the first time in extracting PDE-5 inhibitor western drugs in a sample, IDA-MS high resolution mass spectrum technology is used to quickly screen and analyze the illegally-added male aphrodisiac PDE-5 inhibitor western drugs according to accurate parent ion and fragment molecular weights of primary mass spectrum and secondary mass spectrum as well as isotopic peak data, unknown PDE-5 inhibitor western drug ingredients are quantitatively identified without standards, and the multi-flux detection problem due to the quantity of targets is solved.

Description

technical field [0001] The invention relates to the technical field of detection of unknown ingredients in food, in particular to a method for rapid screening and detection of non-target substances of PDE-5 inhibitor prohibited drugs in health food. Background technique [0002] Health food is a common name in our country's traditional habits, and it is called functional food academically. People's diet has evolved from the traditional concept of nutrition that generally solves hunger and food and clothing to a new concept of nutrition that is beneficial to health and health, which promotes the birth and development of functional foods. The concept of functional food was first proposed by Japan in the early 1980s. Functional food has been developed for more than 30 years. The definition of health (functional) food by the European Union; health food means that if it can be well confirmed that a food can not only provide the usual nutritional effect, but also beneficially aff...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 王志元丁博陈文锐谢建军曾广丰吴俊辉
Owner INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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