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Acinetobacter and application of acinetobacter in removal of nitrogen and phosphorus from wastewater

A technology of Acinetobacter, nitrogen and phosphorus, applied in the field of environmental microorganisms, can solve the problems of low SND efficiency, difficulty in achieving high removal efficiency of N and P, etc., and achieve the effect of high removal rate, wide temperature range and great application potential

Inactive Publication Date: 2016-05-18
WENZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to realize the simultaneous removal of N and P in wastewater, ZengR.J. explored the combination of simultaneous nitrification-denitrification (SND) and anaerobic-anoxic enhanced biological phosphorus removal, and found that the removal of phosphorus is mainly through the use of O by PAOs. 2 It is realized as an electron acceptor to oxidize intracellular PHAs, but the increase of oxygen concentration reduces the efficiency of SND, and it is difficult to achieve high removal efficiency of N and P under the same conditions (ZengR.J., etal. Biotechnol. Bioeng., 2003, 84(2):170-178); and other similar research reports on the simultaneous removal of N and P are achieved through anaerobic and aerobic stages (Satoshi Tsuneda, etal. Biochemical Engineering Journal, 2006, 27: 191-196)

Method used

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  • Acinetobacter and application of acinetobacter in removal of nitrogen and phosphorus from wastewater
  • Acinetobacter and application of acinetobacter in removal of nitrogen and phosphorus from wastewater
  • Acinetobacter and application of acinetobacter in removal of nitrogen and phosphorus from wastewater

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1: Isolation and identification of heterotrophic nitrifying-aerobic denitrifying phosphorus accumulating bacteria

[0042] The sample is activated sludge taken from Wenzhou Xipian Sewage Treatment Plant. A certain amount of activated sludge was inoculated into the enrichment medium ((NH 4 ) 2 SO 4 1g, sodium citrate 5g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O0.10g, CaCl 2 2H 2 O0.10g, FeSO 4 0.02g, nutrient solution 1mL, H 2 O1000mL, 1mol / L NaOH aqueous solution and / or 1mol / L HCl to adjust the pH to 7.0; the nutrient solution is an aqueous solution (g / L) containing the following concentration components: EDTA0.35, ZnSO 4 ·7H 2 O0.20, CuSO 4 ·5H 2 O0.10, MnSO 4 ·7H 2 O0.20, Co(NO 3 ) 2 ·6H 2 O0.09, H 3 BO 3 0.10, Na 2 MoO 4 0.10) Carry out enrichment culture at 25°C and 150r / min for 2 to 3 days, and then transfer to a new enrichment medium to continue enrichment culture after bacteria grow, repeating this 4 to 5 times. Then spread the appropriately d...

Embodiment 2

[0056] Example 2: Nitrogen removal characteristics of Acinetobacter WZUF26 with heterotrophic nitrification-aerobic denitrification and phosphorus accumulation

[0057] Inoculate the preserved strain (1.5mL frozen tube thawed bacteria solution) into a 500mL Erlenmeyer flask filled with 100mL activation medium (the formula is the same as that of enrichment medium in Example 1), and incubate at 30°C, 150~160r / min for 24h , centrifuged at 5000r / min for 10min, washed twice with sterile water, and then prepared with sterile water to prepare a bacterial suspension (OD 680 =0.900~1.000); then transfer to 100mL artificial NH at a volume ratio of 5% 4 + -N sewage or artificial NO 3 - -N sewage or artificial NO 2 - -N sewage in a 250mL Erlenmeyer flask, cultivated at a certain temperature and rotating speed for 48h, then centrifuged at 5000r / min for 10min to obtain a supernatant, and carried out relevant determination and analysis on the supernatant.

[0058] The present invention...

Embodiment 3

[0102] Embodiment three: process of removing nitrogen from artificial nitrogen sewage by Acinetobacter WZUF26

[0103] Acinetobacter WZUF26 bacterium suspension is obtained according to the embodiment two method, transfers to be equipped with 200mL artificial NH by 5% volume ratio 4 + -N sewage ((NH 4 ) 2 SO 4 0.94g, sodium citrate dihydrate 8.17g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O0.10g, CaCl 2 2H 2 O0.10g, FeSO 4 0.02g, nutrient solution 1mL, H 2 O1000mL, 1mol / L NaOH aqueous solution and / or 1mol / L HCl to adjust the pH to 7.0; the nutrient solution is an aqueous solution (g / L) containing the following concentration components: EDTA0.35, ZnSO 4 ·7H 2 O0.20, CuSO 4 ·5H 2 O0.10, MnSO 4 ·7H 2 O0.20, Co(NO 3 ) 2 ·6H 2 O0.09, H 3 BO 3 0.10, Na 2 MoO 4 0.10), artificial NO 3 - -N sewage ((KNO 3 0.72g, sodium citrate dihydrate 4.08g, K 2 HPO 4 0.5g, MgSO 4 ·7H 2 O0.10g, CaCl 2 2H 2 O0.10g, FeSO 4 0.02g, nutrient solution 1mL, H 2 O1000mL, 1mol / L NaOH aqu...

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Abstract

The invention discloses acinetobacter with functions of heterotrophic nitrification-aerobic denitrification and phosphorus accumulation and an application of the acinetobacter in removal of nitrogen and phosphorus from wastewater. The strain is acinetobacter sp. WZUF26 and is preserved in CGMCC (China General Microbiological Culture Collection Center) in the register serial number CGMCC NO.9045. The proper temperature at which the strain removes nitrogen and phosphorus in the nitrogen-phosphorus wastewater is 15-30 DEG C, and the strain has a wide temperature adaptation range; under appropriate conditions, the removal rates of the strain for NH4<+>-N, NO3<->-N, NO2<->-N and P in artificial nitrogen-phosphorus sewage are 8.16 mg / (L*h), 6.02 mg / (L*h), 10.76 mg / (L*h) and 0.39 mg / (L*h) respectively, and the strain has a high removal rate. The strain can synchronously remove nitrogen and phosphorus in the same reactor under an aerobic condition and has great application potential in removal of nitrogen and phosphorus in actual wastewater.

Description

technical field [0001] The invention belongs to the technical field of environmental microorganisms, and specifically relates to a heterotrophic nitrification-aerobic denitrification and phosphorus-accumulating Acinetobacter strain and its application potential in removing nitrogen and phosphorus from nitrogen and phosphorus sewage. Background technique [0002] The eutrophication of the water environment and the excessive growth of algae are one of the main problems causing the deterioration of water quality. P and N are important factors leading to eutrophication of the water body. The main sources of pollution are domestic, industrial and agricultural wastewater. Therefore, the main The purpose is to remove P and N. [0003] Traditional biological nitrogen removal is based on the combination of autotrophic nitrification and anaerobic denitrification. Many heterotrophic nitrifying-aerobic denitrifying bacteria such as ZHENGMaosheng, etal.BioresourceTechnology, 2014,162:80...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C12N1/205C12R2001/01
Inventor 周茂洪赵肖为叶海仁
Owner WENZHOU UNIVERSITY
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