Total bilirubin detection kit

A detection kit and a technology for total bilirubin, which are applied in the field of biomedicine, can solve the problems of being unsuitable for automatic and rapid measurement, weak anti-interference ability, and low measurement precision, and achieve convenient clinical application, rapid response, and measurement precision. high effect

Inactive Publication Date: 2016-05-18
VISION BIOLOGICAL TECH HEFEI CO LTD
4 Cites 4 Cited by

AI-Extracted Technical Summary

Problems solved by technology

[0007] However, all of the above bilirubin testing methods generally have shortcomings such as low measurement precision, weak anti-interf...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Abstract

The invention relates to a total bilirubin detection kit. A first container and a second container are arranged inside the detection kit. A first reagent is contained inside the first container. A second reagent is contained inside the second container. The volume of the first reagent is five times that of the second reagent. The first reagent is composed of 110-150 mmol/L of a Tris-HCl buffer solution, 3-8 mmol/L of a surface active agent, 2-5 wt% of a corrosion remover, 20-60 KU/L of vitamin C oxidase and 2-5 wt% of Triton-x-100. The second reagent is composed of 50-100 mmol/L of a phosphate buffer solution, 1-10 mmol/L of sodium metavanadate, 2-5 wt% of a corrosion remover, 2-5 wt% of a freeze-drying protective additive, 100-200 mmol/L of NaCl, 0.3-0.6 g/L of sodium azide and 1-1.5 ml/L of sulfuric acid. The detection kit has the advantages of being high in detection precision, high in anti-jamming capability, suitable for automatic and rapid detection, and capable of creating favorable conditions for clinic conventional detection and application.

Application Domain

Technology Topic

Examples

  • Experimental program(3)

Example Embodiment

[0017] Example 1
[0018] The total bilirubin detection kit of this embodiment contains a first container and a second container. The first container contains a first reagent. The second container contains a second reagent. And the volume of the first reagent is five times the volume of the second reagent.
[0019] Wherein, the composition of the first reagent is: Tris-HCl buffer 110mmol/L; surfactant 3mmol/L; preservative 2wt%; vitamin C oxidase 20KU/L; Tritonx-1002wt%.
[0020] The composition of the second reagent is: phosphate buffer 50mmol/L; sodium metavanadate 1mmol/L; preservative 2wt%; freeze-dried protective agent 2wt%; NaCl100mmol/L; sodium azide 0.3g/L; sulfuric acid 1ml /L.
[0021] And, wherein, the preservative is potassium sorbate.

Example Embodiment

[0022] Example 2
[0023] The total bilirubin detection kit of this embodiment contains a first container and a second container. The first container contains a first reagent. The second container contains a second reagent. And the volume of the first reagent is five times the volume of the second reagent.
[0024] Wherein, different from Example 1, in this example, the composition of the first reagent is: Tris-HCl buffer 150mmol/L; surfactant 8mmol/L; preservative 5wt%; vitamin C oxidase 60KU/L L; Tritonx-1005wt%.
[0025] At the same time, the composition of the second reagent is: phosphate buffer 100mmol/L; sodium metavanadate 10mmol/L; preservative 5wt%; freeze-dried protective agent 5wt%; NaCl 200mmol/L; sodium azide 0.6g/L; Sulfuric acid 1.5ml/L.
[0026] And, wherein, the preservative is sodium benzoate.

Example Embodiment

[0027] Example 3
[0028] The total bilirubin detection kit of this embodiment contains a first container and a second container. The first container contains a first reagent. The second container contains a second reagent. And the volume of the first reagent is five times the volume of the second reagent.
[0029] Among them, different from Example 1 and Example 2, in this example, the composition of the first reagent is: Tris-HCl buffer 130mmol/L; surfactant 5mmol/L; preservative 4wt%; vitamin C Oxidase 40KU/L; Tritonx-1004wt%.
[0030] At the same time, the composition of the second reagent is: phosphate buffer 80mmol/L; sodium metavanadate 5mmol/L; preservative 4wt%; freeze-dried protective agent 4wt%; NaCl150mmol/L; sodium azide 0.5g/L; Sulfuric acid 1.3ml/L.
[0031] And, wherein, the preservative is sodium nitrite.
[0032] Using the total bilirubin detection kit of the present invention to test patients, it is found that compared with the existing test reagents, the test speed is faster, the accuracy is higher, the display is clearer, and it is easy to store and has a long shelf life; Moreover, the anti-interference ability is strong and more stable.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

no PUM

Description & Claims & Application Information

We can also present the details of the Description, Claims and Application information to help users get a comprehensive understanding of the technical details of the patent, such as background art, summary of invention, brief description of drawings, description of embodiments, and other original content. On the other hand, users can also determine the specific scope of protection of the technology through the list of claims; as well as understand the changes in the life cycle of the technology with the presentation of the patent timeline. Login to view more.
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Similar technology patents

Thermochromic aluminum silicate glass and preparation method thereof

InactiveCN108558203AGood color renderingFast color change response timeAlkali metalAluminum element
Owner:(CNBM) BENGBU DESIGN & RES INST FOR GLASS IND CO LTD

Semiconductor Illuminating Device

ActiveUS20180033921A1Good color renderingGood color saturationSolid-state devicesLuminescent compositionsColor temperatureRadiation
Owner:OSRAM OLED

Classification and recommendation of technical efficacy words

  • Good color rendering
  • Rapid response

Red double-perovskite fluorescent powder for white-light LEDs and preparation method of red double-perovskite fluorescent powder

ActiveCN102634340AGood color renderingHigh emission intensityGas discharge lamp usageLuminescent compositionsRare-earth elementIon
Owner:武汉视美乐激光显示发展有限公司

Fluorescent material with molybdate and tungstate as matrixes as well as preparation method and application thereof

InactiveCN102199427AHigh emission intensityGood color renderingGas discharge lamp usageLuminescent compositionsYttriumTungstate
Owner:JINAN UNIVERSITY

Analytical aid

ActiveUS20060247555A1Rapid responseQuick eliminationCatheterDiagnostic recording/measuringEngineeringApplication site
Owner:ROCHE DIABETES CARE INC

Image display

InactiveUS20050259068A1Rapid responseSimple and inexpensive constructionStatic indicating devicesPhysicsHigh potential
Owner:BRIDGESTONE CORP

User interface for accessing messages

InactiveUS20130018945A1Rapid responseImprove playback qualityError detection/correctionMultiple digital computer combinationsEmbedded applicationsFacsimile
Owner:CALLWAVE COMM
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products