Total bilirubin detection kit

A detection kit and a technology for total bilirubin, which are applied in the field of biomedicine, can solve the problems of being unsuitable for automatic and rapid measurement, weak anti-interference ability, and low measurement precision, and achieve convenient clinical application, rapid response, and measurement precision. high effect

Inactive Publication Date: 2016-05-18
VISION BIOLOGICAL TECH HEFEI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, all of the above bilirubin testing methods generally have shortcomings such as low measurement precision, weak anti-interf

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0017] Example 1

[0018] The total bilirubin detection kit of this embodiment contains a first container and a second container. The first container contains a first reagent. The second container contains a second reagent. And the volume of the first reagent is five times the volume of the second reagent.

[0019] Wherein, the composition of the first reagent is: Tris-HCl buffer 110mmol / L; surfactant 3mmol / L; preservative 2wt%; vitamin C oxidase 20KU / L; Tritonx-1002wt%.

[0020] The composition of the second reagent is: phosphate buffer 50mmol / L; sodium metavanadate 1mmol / L; preservative 2wt%; freeze-dried protective agent 2wt%; NaCl100mmol / L; sodium azide 0.3g / L; sulfuric acid 1ml / L.

[0021] And, wherein, the preservative is potassium sorbate.

Example Embodiment

[0022] Example 2

[0023] The total bilirubin detection kit of this embodiment contains a first container and a second container. The first container contains a first reagent. The second container contains a second reagent. And the volume of the first reagent is five times the volume of the second reagent.

[0024] Wherein, different from Example 1, in this example, the composition of the first reagent is: Tris-HCl buffer 150mmol / L; surfactant 8mmol / L; preservative 5wt%; vitamin C oxidase 60KU / L L; Tritonx-1005wt%.

[0025] At the same time, the composition of the second reagent is: phosphate buffer 100mmol / L; sodium metavanadate 10mmol / L; preservative 5wt%; freeze-dried protective agent 5wt%; NaCl 200mmol / L; sodium azide 0.6g / L; Sulfuric acid 1.5ml / L.

[0026] And, wherein, the preservative is sodium benzoate.

Example Embodiment

[0027] Example 3

[0028] The total bilirubin detection kit of this embodiment contains a first container and a second container. The first container contains a first reagent. The second container contains a second reagent. And the volume of the first reagent is five times the volume of the second reagent.

[0029] Among them, different from Example 1 and Example 2, in this example, the composition of the first reagent is: Tris-HCl buffer 130mmol / L; surfactant 5mmol / L; preservative 4wt%; vitamin C Oxidase 40KU / L; Tritonx-1004wt%.

[0030] At the same time, the composition of the second reagent is: phosphate buffer 80mmol / L; sodium metavanadate 5mmol / L; preservative 4wt%; freeze-dried protective agent 4wt%; NaCl150mmol / L; sodium azide 0.5g / L; Sulfuric acid 1.3ml / L.

[0031] And, wherein, the preservative is sodium nitrite.

[0032] Using the total bilirubin detection kit of the present invention to test patients, it is found that compared with the existing test reagents, the test sp...

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PUM

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Abstract

The invention relates to a total bilirubin detection kit. A first container and a second container are arranged inside the detection kit. A first reagent is contained inside the first container. A second reagent is contained inside the second container. The volume of the first reagent is five times that of the second reagent. The first reagent is composed of 110-150 mmol/L of a Tris-HCl buffer solution, 3-8 mmol/L of a surface active agent, 2-5 wt% of a corrosion remover, 20-60 KU/L of vitamin C oxidase and 2-5 wt% of Triton-x-100. The second reagent is composed of 50-100 mmol/L of a phosphate buffer solution, 1-10 mmol/L of sodium metavanadate, 2-5 wt% of a corrosion remover, 2-5 wt% of a freeze-drying protective additive, 100-200 mmol/L of NaCl, 0.3-0.6 g/L of sodium azide and 1-1.5 ml/L of sulfuric acid. The detection kit has the advantages of being high in detection precision, high in anti-jamming capability, suitable for automatic and rapid detection, and capable of creating favorable conditions for clinic conventional detection and application.

Description

Technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a detection kit, in particular to a total bilirubin detection kit. Background technique [0002] Increased bilirubin is seen in acute icteric hepatitis, acute yellow liver necrosis, chronic active hepatitis, liver cirrhosis, hemolytic jaundice, blood group incompatible transfusion reactions, neonatal jaundice, cholelithiasis, liver cancer, pancreatic head cancer, etc. Low bilirubin is seen in iron deficiency anemia, which is an anemia caused by the lack of iron in the body that affects the synthesis of hemoglobin. The symptoms of iron-deficiency anemia are yellowish or pale complexion, but whether or not iron-deficiency anemia needs to be further checked, red blood cell morphology, serum iron, and serum ferritin are checked. If an anorexic person is deficient in zinc, total bilirubin will also be low. [0003] Direct bilirubin determination is one of the commonly used items in clini...

Claims

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Application Information

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IPC IPC(8): C12Q1/26
CPCC12Q1/26
Inventor 程明
Owner VISION BIOLOGICAL TECH HEFEI CO LTD
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