Total bilirubin detection kit

Abstract

The invention relates to a total bilirubin detection kit. A first container and a second container are arranged inside the detection kit. A first reagent is contained inside the first container. A second reagent is contained inside the second container. The volume of the first reagent is five times that of the second reagent. The first reagent is composed of 110-150 mmol / L of a Tris-HCl buffer solution, 3-8 mmol / L of a surface active agent, 2-5 wt% of a corrosion remover, 20-60 KU / L of vitamin C oxidase and 2-5 wt% of Triton-x-100. The second reagent is composed of 50-100 mmol / L of a phosphate buffer solution, 1-10 mmol / L of sodium metavanadate, 2-5 wt% of a corrosion remover, 2-5 wt% of a freeze-drying protective additive, 100-200 mmol / L of NaCl, 0.3-0.6 g / L of sodium azide and 1-1.5 ml / L of sulfuric acid. The detection kit has the advantages of being high in detection precision, high in anti-jamming capability, suitable for automatic and rapid detection, and capable of creating favorable conditions for clinic conventional detection and application.

Description

Technical field [0001] The invention belongs to the technical field of biomedicine, and relates to a detection kit, in particular to a total bilirubin detection kit. Background technique [0002] Increased bilirubin is seen in acute icteric hepatitis, acute yellow liver necrosis, chronic active hepatitis, liver cirrhosis, hemolytic jaundice, blood group incompatible transfusion reactions, neonatal jaundice, cholelithiasis, liver cancer, pancreatic head cancer, etc. Low bilirubin is seen in iron deficiency anemia, which is an anemia caused by the lack of iron in the body that affects the synthesis of hemoglobin. The symptoms of iron-deficiency anemia are yellowish or pale complexion, but whether or not iron-deficiency anemia needs to be further checked, red blood cell morphology, serum iron, and serum ferritin are checked. If an anorexic person is deficient in zinc, total bilirubin will also be low. [0003] Direct bilirubin determination is one of the commonly used items in clini...

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Problems solved by technology

[0007] However, all of the above bilirubin testing methods generally have shortcomings such as low measurement precision, weak anti-interference ability, and unsuitability for automatic rapid measurement, which is not conducive to the application of clinical routine detection.