Method of extracting and detecting L-cysteine in rice seedling tissues

Abstract

The invention discloses a method of extracting and detecting L-cysteine in rice seedling tissues. The method comprises the following steps of S1, sampling including extracting leaf or root tissues ofrice seedlings, adding in liquid nitrogen and grinding the tissues into powder, extraction including acquiring 0.1-0.2 g of the powder prepared in S1, adding in 1.0-5.0 ml of glacial acetic acid solution for grinding for 5-10 min, and sequentially adding 0.5-2.0 ml of trichloroacetic acid solution and 0.5-2.0 ml of ethanol solution for soaking for 1-5 min to obtain a ground solution mixture, and S3, collection including performing centrifugation on the ground solution mixture obtained in S2 to obtain supernatant, namely, an extract solution containing L-cysteine. Hexadecyl trimethyl ammonium bromide and 1-chloro-2, 4-dinitrobenzene are added into the extracting solution containing L-cysteine for thermostatic reaction at 90-110 DEG C, after reaction is completed, cooling is performed, and colorimetric analysis or absorption photometry is implemented to measure the content of L-cysteine.

Description

technical field [0001] The invention relates to the technical field of plant extraction, in particular to an extraction and detection method of L-cysteine ​​in rice seedling tissue. Background technique [0002] L-cysteine ​​is a common amino acid widely found in animals and plants, and it is widely found in many glutathione and proteins. L-cysteine ​​has the function of relieving and repairing the damage caused by radiation to the human body. It is an important nutrient for maintaining the normal function of the liver. It has a good detoxification effect on formaldehyde, acetaldehyde, lead, cadmium and other poisons. Therefore, in the daily diet, it is meaningful for human health to ensure a certain amount of L-cysteine ​​intake every day. Rice is the staple food of 60% of the population in my country. If the content of L-cysteine ​​in rice can be increased, it will be of great significance to improve the national health of our country, especially for rice at the seedling ...

AI Technical Summary

Problems solved by technology

At present, in the field of biology and medicine, L-cysteine ​​is mostly used in derivatized amino acid analyzers or high-performance liquid chromatography, etc., but the sample pretreatment in the detection process is complicated, and the detection equipment is expensive, so it is difficult to use it in the rice research process. On-site and large-sample-volume testing

[0005] Colorimetric detection method is a relatively commonly used rapid detection method. L-cysteine ​​is one of the few sulfur-containing α-amino acids. It is dark red when it meets sodium nitroferricyanide with -SH, but due to The sodium nitroferricyanide solution itself is dark red, which makes the color reaction more disturbed

Images