Bacillus laterosporus wettable powder preparation method

A technology of Bacillus lateralis and wettable powder, which is applied in the field of preparation of wettable powder of Bacillus lateralis, and can solve the problems such as spore germination agent, high suspension rate, short wetting time, etc. of Bacillus lateralis that have not been publicly reported, Achieve the effect of increasing the germination rate and germination rate, reducing the probability of germination, and reducing the amount of spores

Inactive Publication Date: 2016-05-25
广西云发生物科技有限公司
View PDF6 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] At the present stage, there is no public report on the spore germination agent of Bacillus lateralis, and there is no report on the research of adding a spore germination agent to the wettable powder of Bacillus lateralis
[0011] In addition, due to the compatibility of different bacillus with wetting agents, dispersants and other additives, the carrier has different adsorption properties to various bacillus, and the existing formula or preparation process of bacillus wettable powder cannot be used Preparation of wettable powder of Bacillus lateralis containing spore germination agent; the adsorption amount of effective bacteria in the wettable powder, th

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Bacillus laterosporus wettable powder preparation method
  • Bacillus laterosporus wettable powder preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] A wettable powder of Bacillus lateralis consists of the following components by weight: 60 parts of fermentation broth of Bacillus lateralis; filler: 25 parts of diatomaceous earth; wetting agent: 2 parts of sodium lauryl sulfate; dispersant: carboxymethyl 0.6 parts of sodium cellulose; spore germination agent: 0.3 parts of L-alanine, 0.3 parts of inosine; UV protection agent: 1.5 parts of trehalose, 0.8 parts of ascorbic acid; dry protection agent: 3 parts of skimmed milk powder, 0.8 parts of sucrose.

[0034] The preparation method of this Bacillus lateralis sporogenes wettable powder consists of the following steps:

[0035] One, the preparation of Bacillus lateralosporus fermented liquid

[0036] Take out the strain preservation tube, scratch the plate with LB solid medium for recovery, and incubate at 37°C for 48 hours. Pick a single colony under the plate and inoculate it on the LB solid medium in the eggplant bottle, spread it all over the eggplant bottle, and c...

Embodiment 2

[0045] The effect of using wettable powder of Bacillus lateralis as microbial fertilizer and pesticide in flue-cured tobacco

[0046] 1. Test site: a farmer's planting site in Shizi Village, Tantou Town, Longhui County, Hunan Province

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The present invention relates to the field of bio-pesticides and microbial fertilizers, and specifically discloses spore-germinator-containing bacillus laterosporus wettable powder and a preparation method thereof, the spore-germinator-containing bacillus laterosporus wettable powder comprises the following components by weight: 40-70 parts of bacillus laterosporus fermentation broth; 20-30 parts of diatomaceous earth as a filler; 1-4 parts of sodium lauryl sulfate as a wetting agent; 0.2-1 part of sodium carboxymethyl cellulose as a dispersant; 0.1-0.5 part of L-alanine as a spore germinator; 0.1-0.5 part of inosine as the spore germinator; 0.5-2 parts of trehalose as an UV protective agent; 0.2-1 part of ascorbic acid as the UV protective agent; 1-4 parts of skimmed milk powder as a drying protective agent and 0.2-1 part of cane sugar as the drying protective agent. After application of the spore-germinator-containing bacillus laterosporus wettable powder into a large field, spore germination speed is faster, germination rate is high, fertilizer effect and plant disease control effect can be significantly improved, the amount of spores can be reduced, and production cost is reduced.

Description

technical field [0001] The invention relates to the technical field of biological pesticides and microbial fertilizers, in particular to a method for preparing a wettable powder of Bacillus lateralis containing a spore germination agent. Background technique [0002] A large number of chemical pesticide preparations are used in our country every year, which can easily cause excessive pesticide residues and bio-enrichment, which not only pollutes the ecological environment, but also affects human health. However, biopesticide has few side effects and is harmless to humans. It is a kind of green pesticide with broad prospects and has increasingly become a trend in the development of global pesticides. [0003] Bacillus is currently the most researched microorganism in biological pesticides and microbial fertilizers. It has broad-spectrum antibacterial activity and strong resistance to stress. Its vegetative cells can reproduce and colonize on the surface of fungal pathogenic m...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01N25/14A01N63/00A01P21/00A01P3/00
CPCA01N25/14A01N63/00
Inventor 胡艳晖
Owner 广西云发生物科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products