Bacillus laterosporus wettable powder preparation method

Abstract

The present invention relates to the field of bio-pesticides and microbial fertilizers, and specifically discloses spore-germinator-containing bacillus laterosporus wettable powder and a preparation method thereof, the spore-germinator-containing bacillus laterosporus wettable powder comprises the following components by weight: 40-70 parts of bacillus laterosporus fermentation broth; 20-30 parts of diatomaceous earth as a filler; 1-4 parts of sodium lauryl sulfate as a wetting agent; 0.2-1 part of sodium carboxymethyl cellulose as a dispersant; 0.1-0.5 part of L-alanine as a spore germinator; 0.1-0.5 part of inosine as the spore germinator; 0.5-2 parts of trehalose as an UV protective agent; 0.2-1 part of ascorbic acid as the UV protective agent; 1-4 parts of skimmed milk powder as a drying protective agent and 0.2-1 part of cane sugar as the drying protective agent. After application of the spore-germinator-containing bacillus laterosporus wettable powder into a large field, spore germination speed is faster, germination rate is high, fertilizer effect and plant disease control effect can be significantly improved, the amount of spores can be reduced, and production cost is reduced.

Description

technical field [0001] The invention relates to the technical field of biological pesticides and microbial fertilizers, in particular to a method for preparing a wettable powder of Bacillus lateralis containing a spore germination agent. Background technique [0002] A large number of chemical pesticide preparations are used in our country every year, which can easily cause excessive pesticide residues and bio-enrichment, which not only pollutes the ecological environment, but also affects human health. However, biopesticide has few side effects and is harmless to humans. It is a kind of green pesticide with broad prospects and has increasingly become a trend in the development of global pesticides. [0003] Bacillus is currently the most researched microorganism in biological pesticides and microbial fertilizers. It has broad-spectrum antibacterial activity and strong resistance to stress. Its vegetative cells can reproduce and colonize on the surface of fungal pathogenic m...

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Problems solved by technology

[0010] At the present stage, there is no public report on the spore germination agent of Bacillus lateralis, and there is no report on the research of adding a spore germination agent to the wettable powder of Bacillus lateralis

[0011] In addition, due to the compatibility of different bacillus with wetting agents, dispersants and other additives, the carrier has different adsorption properties to various bacillus, and the existing formula or preparation process of bacillus wettable powder cannot be used Preparation of wettable powder of Bacillus lateralis containing spore germination agent; the adsorption amount of effective bacteria in the wettable powder, the survival rate, the control effect on harmful bacteria and the stability of the wettable powder dose are quite different, and the whole wettable powder The preparation method must fully consider the adsorption capacity of each carrier, the protective effect on the drying process of Bacillus lateralis, the influence of wetting agent and dispersant on the suspension rate of wettable powder, and the wetting time, and comprehensively screen the spore germination agent In order to obtain a wettable powder with short wetting time, high suspension rate, long storage time of spores, rapid germination of spores after use, and good control effect of Bacillus lateralis

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