Unlock instant, AI-driven research and patent intelligence for your innovation.

Prunella vulgaris homogeneous polysaccharide as well as preparation method and application thereof

A technology of Prunella vulgaris and polysaccharides, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, and plant raw materials, etc. It can solve problems that do not involve polysaccharide molecular weight, purity, and structural analysis, and achieve strong anti-herpes virus activity and high purity , the effect of high polysaccharide content

Active Publication Date: 2016-05-25
INCREASEPHARM HENGQIN INST CO LTD
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the patent application does not involve research on the molecular weight, purity and structural analysis of the polysaccharides

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Prunella vulgaris homogeneous polysaccharide as well as preparation method and application thereof
  • Prunella vulgaris homogeneous polysaccharide as well as preparation method and application thereof
  • Prunella vulgaris homogeneous polysaccharide as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation and analysis of embodiment 1 Prunella vulgaris homogeneous polysaccharide PSP-2B

[0039] 1. Preparation

[0040] 【Experimental Materials】

[0041] Prunella vulgaris, heating mantle, condenser tube, round bottom flask, MilliporeLabscale TFF ultrafiltration system, Lambda35 UV spectrophotometer, PerkinElmer SpectrumOne Fourier transform infrared spectrometer, AKTApurifier100 series protein purification instrument, Agilent1100 series high performance gel permeation chromatography, PolarimeterModel341 polarimeter, WatersHClass ultra-high performance liquid chromatography, phenol, carbazole, barium sulfate, gelatin, sulfuric acid, 1-phenyl-3-methyl-5-pyrazolone, etc.

[0042] 【experimental method】

[0043]Decoct 500g of Prunella vulgaris fruit ears for 3 times, add 20 times of water each time, 0.5h each time, pass the water extract through a 0.45μm water membrane microfiltration, collect the filtrate, concentrate the filtrate under reduced pressure at 50°C, an...

Embodiment 2

[0095] Preparation and analysis of embodiment 2 Prunella vulgaris homogeneous polysaccharide B

[0096] Decoct 500g of Prunella vulgaris fruit ears for 3 times, add 20 times of water each time, 0.5h each time, pass the water extract through a 0.45μm water membrane microfiltration, collect the filtrate, concentrate the filtrate under reduced pressure at 50°C, and dilute the concentrated filtrate in turn Pass through ultrafiltration membranes with a molecular weight cut-off of 30KDa and 100KDa, collect the ultrafiltrate with a molecular weight cut-off greater than 30KDa and less than 100KDa, use the Sevag method to remove free protein 7 times, concentrate to saturation, and take the saturated solution and load it on SephadexG-200 dextran Gel column (1.6×100cm), 1.5ml of each sample loaded, the mobile phase is deionized water, the flow rate is 0.4ml / min, collected in separate tubes, 5ml in each tube, the collected solution is detected by the sulfuric acid-phenol method for polysac...

Embodiment 3

[0100] Preparation and analysis of embodiment 3 Prunella vulgaris homogeneous polysaccharide C

[0101] Decoct 500g of Prunella vulgaris fruit ears for 3 times, add 20 times of water each time, 0.5h each time, pass the water extract through a 0.45μm water membrane microfiltration, collect the filtrate, concentrate the filtrate under reduced pressure at 50°C, and dilute the concentrated filtrate in turn Pass through ultrafiltration membranes with a molecular weight cut-off of 30KDa and 100KDa, collect the ultrafiltrate with a molecular weight cut-off greater than 30KDa and less than 100KDa, use the Sevag method to remove free protein 7 times, concentrate to saturation, and take the saturated solution and load it on SephadexG-200 dextran Gel column (1.6×100cm), 2ml of sample loading each time, the mobile phase is deionized water, the flow rate is 0.4ml / min, collected in separate tubes, 5ml in each tube, the collected solution is detected by the sulfuric acid-phenol method for pol...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
Login to View More

Abstract

The invention discloses prunella vulgaris homogeneous polysaccharide. The total sugar content of the homogeneous polysaccharide is 53-65%; the molecular weight of the homogeneous polysaccharide is 23.4-41.7KDa; and the homogeneous polysaccharide mainly comprises mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose and arabinose. The invention also provides a preparation method and an application of the prunella vulgaris homogeneous polysaccharide. According to the prunella vulgaris homogeneous polysaccharide disclosed by the invention, the polysaccharide content and purity are higher, and the anti-virus activity is stronger; and the preparation technology is also suitable for industrial production.

Description

technical field [0001] The invention relates to the technical field of traditional Chinese medicine extracts, in particular to a plant extract component and its preparation method and application. Background technique [0002] Chinese invention patent application (201410561972.8) discloses a water extract of Prunella vulgaris, the water extract of Prunella vulgaris is prepared by the following method: adding 10 to 22 times the amount of water to the fruit ears of Prunella vulgaris, heating and refluxing for extraction 2 to 4 times, each 0.5~2h, filter, filtrate through 0.45um water membrane microfiltration, collect the filtrate after passing through 0.45um water membrane microfiltration, and then filter the collected filtrate after passing through 0.45um water membrane microfiltration with a molecular weight cut-off of 3~500KDa The ultrafiltration membrane is used to collect the liquid medicine that does not pass through the ultrafiltration membrane, concentrate, and dry. T...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/715A61P31/22A61K36/536
CPCA61K31/715A61K36/536A61K2236/331A61K2236/39A61K2236/51A61K2236/53A61K2236/55
Inventor 徐宏喜周燕马风伟吴蓉孔思远谭红胜
Owner INCREASEPHARM HENGQIN INST CO LTD