A kind of Prunella vulgaris homogeneous polysaccharide and its preparation method and application
A technology of Prunella vulgaris and polysaccharide, which is applied in pharmaceutical formulations, medical preparations containing active ingredients, and plant/algae/fungus/moss components, etc., can solve problems such as molecular weight, purity and structural analysis of polysaccharides that are not involved, and achieve anti-herpes. The effect of strong viral activity, high purity and high polysaccharide content
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Embodiment 1
[0038] Preparation and analysis of embodiment 1 Prunella vulgaris homogeneous polysaccharide PSP-2B
[0039] 1. Preparation
[0040] 【Experimental Materials】
[0041] Prunella vulgaris, heating mantle, condenser, round bottom flask, Millipore Labscale TFF ultrafiltration system, Lambda35 UV spectrophotometer, Perkin Elmer Spectrum One Fourier transform infrared spectrometer, AKTA purifier100 series protein purifier, Agilent 1100 series high performance gel permeation chromatography Instrument, Polarimeter Model 341 polarimeter, Waters H Class ultra-high performance liquid chromatography, phenol, carbazole, barium sulfate, gelatin, sulfuric acid, 1-phenyl-3-methyl-5-pyrazolone, etc.
[0042] 【experimental method】
[0043]Decoct 500g of Prunella vulgaris fruit ears for 3 times, add 20 times of water each time, 0.5h each time, pass the water extract through a 0.45μm water membrane microfiltration, collect the filtrate, concentrate the filtrate under reduced pressure at 50°C, an...
Embodiment 2
[0095] Preparation and analysis of embodiment 2 Prunella vulgaris homogeneous polysaccharide B
[0096] Decoct 500g of Prunella vulgaris fruit ears for 3 times, add 20 times of water each time, 0.5h each time, pass the water extract through a 0.45μm water membrane microfiltration, collect the filtrate, concentrate the filtrate under reduced pressure at 50°C, and dilute the concentrated filtrate in turn Pass through ultrafiltration membranes with a molecular weight cut-off of 30KDa and 100KDa, collect the ultrafiltrate with a molecular weight cut-off greater than 30KDa and less than 100KDa, remove free protein 7 times by the Sevag method, concentrate to saturation, and load the saturated solution on Sephadex G-200 Sephadex Glucose gel column (1.6×100cm), 1.5ml of each sample loaded, the mobile phase is deionized water, the flow rate is 0.4ml / min, collected in separate tubes, 5ml in each tube, the collected solution is detected by the sulfuric acid-phenol method for polysaccharid...
Embodiment 3
[0100] Preparation and analysis of embodiment 3 Prunella vulgaris homogeneous polysaccharide C
[0101] Decoct 500g of Prunella vulgaris fruit ears for 3 times, add 20 times of water each time, 0.5h each time, pass the water extract through a 0.45μm water membrane microfiltration, collect the filtrate, concentrate the filtrate under reduced pressure at 50°C, and dilute the concentrated filtrate in turn Pass through ultrafiltration membranes with a molecular weight cut-off of 30KDa and 100KDa, collect the ultrafiltrate with a molecular weight cut-off greater than 30KDa and less than 100KDa, remove free protein 7 times by the Sevag method, concentrate to saturation, and load the saturated solution on Sephadex G-200 Sephadex Glucose gel column (1.6×100cm), 2ml of sample loading each time, the mobile phase is deionized water, the flow rate is 0.4ml / min, collected in separate tubes, 5ml in each tube, the collected solution is detected by the sulfuric acid-phenol method for polysacch...
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