Germanium-rich yeast additive adopting rice bran as medium, and preparation method thereof
A medium and additive technology, applied in animal feed, fungi, animal feed, etc., can solve problems such as threats to livestock and poultry and human health, unreasonable use of additives, ecological environment impact, etc., to improve animal immunity, avoid Drug resistance, the effect of enhancing disease resistance
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Embodiment 1
[0026] A preparation method of a germanium-rich yeast additive using rice bran as a medium, comprising the following steps,
[0027] Weigh 200g of peeled potatoes, cut into pieces, add water and boil for 30min, filter with gauze and collect the filtrate, add 20g of glucose to the filtrate, add water to 1000ml after melting, boil and add 15g of agar in boiling state, and stir until Dissolve completely, make up to 1000ml with distilled water, and carry out high-pressure steam sterilization to obtain the fermentation medium;
[0028] Weigh 200g of peeled potatoes, cut into pieces, add water and boil for 30min, filter with gauze and collect the filtrate, add 20g of glucose to the filtrate, add water to 1000ml after melting, boil and add 15g of agar in boiling state, and stir until Dissolve completely, make up to 1000ml with distilled water, sterilize with high pressure steam to obtain seed medium, then pick a ring of yeast from the inclined storage tube under aseptic conditions, i...
Embodiment 2
[0031] A preparation method of a germanium-rich yeast additive using rice bran as a medium, comprising the following steps,
[0032] Weigh 250g of peeled potatoes, cut into pieces, add water and boil for 40min, filter with gauze and collect the filtrate, add 25g of glucose to the filtrate, add water to 1000ml after melting, boil and add 18g of agar in boiling state, and stir until Dissolve completely, make up to 1000ml with distilled water, and carry out high-pressure steam sterilization to obtain the fermentation medium;
[0033] Weigh 250g of peeled potatoes, cut into pieces, add water and boil for 40min, filter with gauze and collect the filtrate, add 25g of glucose to the filtrate, add water to 1000ml after melting, boil and add 18g of agar in boiling state, and stir until Dissolve completely, make up to 1000ml with distilled water, sterilize with high pressure steam to obtain seed medium, then pick a ring of yeast from the inclined storage tube under aseptic conditions, i...
Embodiment 3
[0036] A preparation method of a germanium-rich yeast additive using rice bran as a medium, comprising the following steps,
[0037] Weigh 150g of peeled potatoes, cut into pieces, add water and boil for 20min, filter with gauze and collect the filtrate, add 22g of glucose to the filtrate, add water to 1000ml after melting, boil and add 14g of agar in boiling state, and stir until Dissolve completely, make up to 1000ml with distilled water, and carry out high-pressure steam sterilization to obtain the fermentation medium;
[0038] Weigh 200g of peeled potatoes, cut into pieces, add water and boil for 30min, filter with gauze and collect the filtrate, add 22g of glucose to the filtrate, add water to 1000ml after melting, boil and add 16g of agar in boiling state, and stir until Dissolve completely, make up to 1000ml with distilled water, sterilize with high pressure steam to obtain seed medium, then pick a ring of yeast from the inclined storage tube under aseptic conditions, i...
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