Bacterium for effectively degrading glyphosate

A technology of glyphosate, bacteria, applied in the field of environmental biology

Inactive Publication Date: 2016-05-25
WUHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although there is no research report on the carcinogenicity of glyphosate in my country, its potential toxic

Method used

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  • Bacterium for effectively degrading glyphosate
  • Bacterium for effectively degrading glyphosate
  • Bacterium for effectively degrading glyphosate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] [Example 1] Screening of efficient glyphosate pesticide degrading bacteria

[0024] The experimental soil and water samples were collected from the rhizosphere and non-rhizosphere soil samples around the new plant of Qinong Chemical Co., Ltd., Qizhou Town, Hubei Province, and the water samples were also collected from 5 drainage outlets of the plant. After the water samples were collected, they were immediately stored in the laboratory at -20°C to kill insects, plant tissues and some viruses in the soil. After 10 hours of treatment, it was thawed at 4°C, and the excess soil samples were stored in a 4°C refrigerator. Take 10g soil sample or 10mL water sample, add 100mL sterilized liquid enrichment medium (containing sterile glass beads) (enrichment medium composition: peptone 10g / L, NaCl1.0g / L, KH 2 PO 3 1.0g / L, pH=7), shaking at 150rpm for 7 days. After culturing for 1 week, take 10 mL of the culture solution in the enriched medium, add them to the enriched medium co...

Embodiment 2

[0025] [Example 2] identification of bacterial strains

[0026] Inoculate the purified strain on solid LB medium (ingredients: tryptone 10g / L, yeast extract 1g / L, NaCl 1g / L, agar 20g / L, pH=7) and culture for 5 days to observe the colony of the strain on the solid plate Morphological characteristics: the colony is round, opaque, the diameter of the mature colony is about 0.8cm, light yellow to yellow, the colony is moist and sticky and easy to pick (such as figure 1 shown).

[0027] The physical and chemical properties of the strains were identified mainly by reference to the Physiological and Biochemical Tests of Berger Bacteria Identification. Record the results in a form.

[0028] Table 1: Physiological and biochemical detection of bacteria YU1-1

[0029]

[0030] Note: "+" indicates a positive result after 5 days of culture, and "-" indicates a negative result

[0031] Bacterial 16SrDNA sequencing included strain DNA extraction, 16SrDNA in vitro amplification (using ...

Embodiment 3

[0032] [Example 3] Degradation rate analysis of bacterial strains to glyphosate

[0033] 1. Construction of degradation kinetic model

[0034] Liquid MSM medium was used, and the final concentration after adding glyphosate pesticide was set at 100mg / L. Before the determination, inoculate the strain in LB liquid medium for activation, activate and cultivate at 180rpm at 37°C for 24 hours, then take 2mL of the activated culture medium and measure the OD 600 Value, then add 2ml of sterile distilled water to make a uniform bacterial suspension, and add it to the medium at a volume ratio of 10%. All the cultures were shaken at 180rpm at 28°C for 24 hours, and the glyphosate content and strain OD in the supernatant were measured every 2 hours. 600 value, the supernatant was diluted, nitrosated to constant volume, filtered, and the residual amount of glyphosate was determined by the reversed-phase high-performance liquid chromatography analysis method. According to the change of th...

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Abstract

The invention discloses a bacterium for effectively degrading glyphosate. The bacterium is called Aquamicrobium defluvii and is marked as YU 1-1, a strain is conserved in the China center for type culture collection in Wuhan University, and the conservation number is CCTCC NO.M2015816. The invention further discloses the glyphosate degradation characteristic and the biodegradation mechanism of the efficient pesticide degrading bacterium. By means of degradation product analysis, glyphosate molecules are first degraded into an intermediate product aminomethyl phosphonic acid (AMPA) and then further degraded into phosphate molecules and methylamine molecules to provide a phosphorus source and a carbon source for microbial growth. Metabolite of glyphosate degraded by the stain is nontoxic and high in environment affinity. The stain has high application value in treatment on water body environment polluted by glyphosate pesticide, treatment on soil environment and comprehensive treatment on current industrial sitepesticide pollution.

Description

technical field [0001] The invention belongs to the technical field of environmental biology, and in particular relates to a bacterium capable of efficiently degrading glyphosate. Background technique [0002] The widespread use of pesticides, in addition to bringing rapid and efficient crop yields to agricultural production, has also caused serious pollution to water bodies, soil and the atmospheric environment, and has also increased product safety issues for agricultural products. At present, the commonly used pesticide remediation methods generally adopt methods such as kiln incineration, thermal desorption and chemical treatment, but there are problems such as high cost, difficult remediation, and easy to cause secondary pollution. The bioremediation method established by using organisms (mainly microorganisms) in water and soil that can use organic pesticides as energy substances and degrade them into non-toxic or low-toxic compounds is recognized as an efficient, safe...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34A62D3/02B09C1/10C12R1/01C02F101/38A62D101/04A62D101/26
CPCA62D3/02A62D2101/04A62D2101/26B09C1/10C02F3/34C02F2101/38C12N1/205C12R2001/01
Inventor 陈兰洲田江余臣磊汪静赵瑞雪赵剑
Owner WUHAN UNIV
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