A preparation method of magnetic-fluorescent functional nano-biocomposite with mycotoxin targeting effect

A mycotoxin and functional nanotechnology, applied in the fields of spectrochemistry, materials science, and nanobiosensing, can solve the problems of difficult modification, poor stability, poor biocompatibility, etc., and achieve the effect of improving stability and reliable detection tools.

Inactive Publication Date: 2018-04-24
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Solve the problems of poor stability, difficult modification, poor biocompatibility, and lack of targeted recognition of mycotoxins in existing multifunctional probes

Method used

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  • A preparation method of magnetic-fluorescent functional nano-biocomposite with mycotoxin targeting effect
  • A preparation method of magnetic-fluorescent functional nano-biocomposite with mycotoxin targeting effect
  • A preparation method of magnetic-fluorescent functional nano-biocomposite with mycotoxin targeting effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Preparation of green fluorescent CdTe QDs aqueous solution:

[0032] Prepare the green fluorescent CdTe QDs aqueous solution according to the existing method. The details are as follows: Weigh 0.0638g tellurium powder and 0.0449g sodium borohydride into a 10mL colorimetric tube, add 4mL water, and pass N 2 After deoxygenation for 15 minutes, it was placed in a refrigerator at 4°C for reaction to obtain a sodium telluride hydride (NaHTe) solution. 0.1142g CdCl 2 ·2.5H 2 Add O and 75μL mercaptopropionic acid to a three-necked flask containing 50mL of secondary water, stir magnetically for 5min under nitrogen protection, adjust the pH of the solution to 8.5 with 2M NaOH, quickly add 2mL of the NaHTe solution prepared above, and continue stirring for 30min at 100℃ After refluxing for 0.5h, green fluorescent CdTe QDs can be prepared. Add 100mL ethanol and stir and then stand for 15min. After centrifugation, dry naturally in the dark, and redisperse the product to obtain 10mg...

Embodiment 2

[0041] (1) Preparation of CdTe QDs aqueous solution emitting yellow fluorescence:

[0042] Prepare the green fluorescent CdTe QDs aqueous solution according to the existing method, and the details are as follows: Weigh 0.0638g tellurium powder and 0.0449g sodium borohydride into a 10mL colorimetric tube, add 4mL ultrapure water, pass N 2 After deoxygenation for 15 minutes, it was placed in a refrigerator at 4°C for reaction to obtain a sodium telluride hydride (NaHTe) solution. 0.1142g CdCl 2 ·2.5H 2 O and 75μL mercaptopropionic acid were added to a three-necked flask containing 50mL of secondary water, magnetically stirred for 5min under nitrogen protection, adjusted the pH of the solution to 8.5 with 2M NaOH, quickly added 2mL of the NaHTe solution prepared above, and continued stirring for 30min, 100℃ Under reflux for 4h, CdTeQDs emitting yellow fluorescence can be prepared. Stop the reaction and return to room temperature, add 100 mL of ethanol and stir and then let it stand ...

Embodiment 3

[0050] (1) Preparation of CdTe QDs aqueous solution emitting orange fluorescence:

[0051] Prepare the green fluorescent CdTe QDs aqueous solution according to the existing method, and the details are as follows: Weigh 0.0638g tellurium powder and 0.0449g sodium borohydride into a 10mL colorimetric tube, add 4mL ultrapure water, pass N 2 After deoxygenation for 15 minutes, it was placed in a refrigerator at 4°C for reaction to obtain a sodium telluride hydride (NaHTe) solution. 0.1142g CdCl 2 ·2.5H 2 O and 75μL mercaptopropionic acid were added to a three-necked flask containing 50mL of secondary water, magnetically stirred for 5min under nitrogen protection, adjusted the pH of the solution to 8.5 with 2M NaOH, quickly added 2mL of the NaHTe solution prepared above, and continued stirring for 30min, 100℃ Under reflux for 8h, CdTeQDs with yellow fluorescence can be prepared. Stop the reaction and return to room temperature, add 100 mL of ethanol and stir and then let it stand for ...

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Abstract

The invention provides a preparation method of a magnetic-fluorescent functional nano bio-compound with mycotoxin targeting effect. The preparation method includes following steps: preparing a water-soluble CdTe quantum dot (CdTe QDs for short) water solution; preparing Fe3O4 magnetic nanosphere dispersion liquid; preparing SiO2-coated Fe3O4 (Fe3O4@SiO2 for short) magnetic nanosphere dispersion liquid; preparing CdTe QDs-coated Fe3O4@SiO2 difunctional nanosphere (Fe3O4@SiO2@CdTe QDs for short) dispersion liquid; preparing Fe3O4@SiO2@CdTe QDs-marked nano bio-compound dispersion liquid with a mycotoxin targeting function. SiO2 shell layer coating is performed on the surfaces of Fe3O4 magnetic nanospheres to prepare Fe3O4@SiO2 magnetic composite spheres, so that stability, chemical modification performance and biocompatibility of a magnetic control carrier are improved effectively.

Description

Technical field [0001] In the fields of materials science, spectrochemistry, nano-biological sensing, etc., the present invention specifically refers to a method for preparing a magnetic-fluorescence functional nano-biological composite with mycotoxin targeting effect. Background technique [0002] Agricultural products are prone to breed or come into contact with toxin-producing molds during growth, harvesting, storage, and processing, which leads to mycotoxins entering the food chain. Ingestion of agricultural products that are heavily contaminated by mycotoxins and foods processed by them can produce a variety of acute poisoning symptoms, such as acute vomiting, hemorrhagic disease, polyneuritis, Estrogen Hyperactivity, etc., the mortality rate is extremely high. It should be pointed out that, unlike foods contaminated with bacterial toxins that darken or emit unpleasant odors, agricultural products contaminated with mycotoxins often have no obvious difference in appearance an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C09K11/88B82Y40/00H01F1/44G01N21/64
CPCB82Y40/00C09K11/883G01N21/6486H01F1/0054H01F1/445
Inventor 王成全钱静黄星奕王坤安克奇华梦娟刘倩郝楠
Owner JIANGSU UNIV
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