Process for preparing natural green persimmon dye based on bacillus subtilis fermentation method and application of obtained dye

A technology of Bacillus subtilis and natural dyes, applied in the field of preparation and application of natural dyes, can solve the problems of complicated operation and difficult to remove sugar, and achieves the effects of avoiding damage, saving processes and having good antibacterial effect.

Pending Publication Date: 2016-06-01
CHANGZHOU UNIV
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AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is: to provide a method for extracting natural dyes of green persimmons, avoiding the problems of "complex operation and difficult removal of sugar" in the process of extracting green persimmon dyes in the prior art, and at the same time provide a method for extracting dyes by this process. Application of persimmon dye

Method used

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  • Process for preparing natural green persimmon dye based on bacillus subtilis fermentation method and application of obtained dye
  • Process for preparing natural green persimmon dye based on bacillus subtilis fermentation method and application of obtained dye
  • Process for preparing natural green persimmon dye based on bacillus subtilis fermentation method and application of obtained dye

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Embodiment 1

[0022] Preparation process of nutrient broth:

[0023] Dissolve 10g of peptone, 3g of beef extract powder, and 5g of sodium chloride in 1000mL of distilled water, and after dissolving, sterilize under high pressure at 121°C for 15 minutes, then lower it to room temperature (25°C) to obtain a nutrient broth;

[0024] The preparation process of Bacillus subtilis experimental bacterial liquid is:

[0025] Take a frozen tube of Bacillus subtilis, after it melts at room temperature (25°C), use an inoculation loop to take the bacterial suspension and inoculate it on a nutrient agar medium plate by streaking method, and incubate at 37°C for 22 hours; Take typical colonies and inoculate them into slant test tubes of nutrient agar medium, incubate at 37°C for 22 hours, store the slant test tubes in the refrigerator (5°C) as preserved bacteria; use an inoculation loop to take the preserved bacteria, and inoculate them by streaking Put it on an agar medium plate, and cultivate it at 37°...

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Abstract

The invention provides a process for preparing natural green persimmon dye based on a bacillus subtilis fermentation method and application of the obtained dye, belongs to the field of preparation and application of the natural dye, and particularly relates to the process for preparing the natural green persimmon dye based on a fermentation method and application of the obtained dye. A saccharomycete fermentation mode is adopted, carbohydrate constituents are completely decomposed and converted into ethyl alcohol while the green persimmon dye is extracted, and the phenomenon of wall sticking, caused by the fact that the proportion of sugar in extract is too high in the following drying process, of a drying machine is avoided. The green persimmon extract is obtained in a bacillus subtilis fermentation environment, and a byproduct generated through bacillus subtilis fermentation and the green persimmon extract coordinate on the aspect of preventing and treating anthracnose. The extract achieves bacterium resistance and ultraviolet resistance properties after dyeing fabric.

Description

technical field [0001] The invention belongs to the field of preparation and application of natural dyes, and in particular relates to a process for preparing green persimmon natural dyes by a fermentation method and the application of the obtained dyes. Background technique [0002] Natural dyes are gradually becoming well known to the public for their unique properties such as natural pollution-free, beneficial to human health, and neutral and soft colors. The natural dyes generally used are all taken from Chinese herbal medicines. During the dyeing and finishing process, the dyes and beneficial components are used to dye the fabric together, which makes the fabric not only have a unique and natural color, but also have additional benefits such as skin care, insect repellent, health care, and life optimization. Features. [0003] Persimmon tree is a temperate deciduous fruit tree of Persimmonaceae berry class. It is native to my country and has a planting history of more ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P1/04D06B3/10A01N65/18A01P3/00C12R1/125
CPCC12P1/04A01N65/00A01N65/18D06B3/10A01N2300/00
Inventor 张冒飞彭勇刚汪媛纪俊玲
Owner CHANGZHOU UNIV
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