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Preparation method of composite polyphosphoester polydopamine biosensor

A technology of biosensor and polydopamine, which is applied in the direction of instruments, scientific instruments, measuring devices, etc., can solve the problems of inability to adhere to cells, poor compatibility between biological devices and biocompatibility, and achieve good biocompatibility

Inactive Publication Date: 2016-06-01
CHANGZHOU YAHUAN ENVIRONMENTAL PROTECTION TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention: Aiming at the problem that a large amount of solvent is needed in the preparation process of the biosensor, and the prepared biological device has poor biocompatibility and cannot effectively adhere to the cells, a biosensor through the shell is provided. Polysaccharide biomaterials are used as the base layer, and horseradish peroxidase is compounded and fixed. Under alkaline conditions, polydopamine can quickly form a film on the surface of various materials, which contains a large number of hydrophilic hydroxyl and amino functional groups, improving the surface of the material. Hydrophilicity and chemical versatility, effectively improving the compatibility of biosensors with the human body

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0016] First, in parts by weight, 5 parts of chitosan powder, 30 parts of acetic acid solution with a mass fraction of 20%, 40 parts of epoxy soybean oil and 25 parts of polysorbate were stirred and mixed for 10 minutes, and then mixed with 0.8mol / L NaOH solution to adjust the pH to 8, heat up to 60°C and react for 2 hours to prepare a chitosan solution; after the heating reaction is completed, nitrogen gas is introduced at 300r / min for 10min, then continue stirring and add the total mass of the chitosan solution 1% dopamine hydrochloride, stirred and reacted for 25 minutes, placed it in 200W ultrasonic vibration treatment for 10 minutes, then continued to stir and reacted for 20 hours; Min under centrifugal treatment 10min, collect mixture and wash 3 times with distilled water, and according to solid-liquid mass ratio 1:5, redisperse in distilled water, prepare chitosan polydopamine suspension; Press chitosan polydopamine suspension and spicy Root peroxidase mass ratio is 8:1...

example 2

[0018] First, in parts by weight, 20 parts of chitosan powder, 20 parts of acetic acid solution with a mass fraction of 20%, 35 parts of epoxy soybean oil and 25 parts of polysorbate were stirred and mixed for 12 minutes, and then mixed with 0.8mol / L NaOH solution to adjust the pH to 9, heat up to 60°C and react for 2.5 hours to prepare a chitosan solution; after the heating reaction is completed, nitrogen gas is introduced at 400r / min for 12min, then continue stirring and add the total amount of chitosan solution 1% dopamine hydrochloride by mass, stirred for 27 minutes, placed it in 250W ultrasonic vibration treatment for 10 minutes, then continued to stir and reacted for 22 hours; after the reaction was completed, stop heating and let it cool naturally to 25°C, then place it in Centrifugal processing under / min for 12min, the mixture was collected and washed 4 times with distilled water, and redispersed in distilled water at a solid-to-liquid mass ratio of 1:5 to prepare chi...

example 3

[0020]First, in parts by weight, 35 parts of chitosan powder, 15 parts of acetic acid solution with a mass fraction of 20%, 30 parts of epoxy soybean oil and 20 parts of polysorbate were stirred and mixed for 15 minutes, and then mixed with 0.8mol / L NaOH solution to adjust the pH to 10, heat up to 60°C and react for 3 hours to prepare a chitosan solution; after the heating reaction is completed, nitrogen gas is introduced at 500r / min for 15min, then continue stirring and add the total mass of the chitosan solution 1% dopamine hydrochloride, stirred and reacted for 30 minutes, placed it in 300W ultrasonic vibration treatment for 10 minutes, then continued to stir and reacted for 24 hours; Min under centrifugal treatment 15min, collect the mixture and wash 5 times with distilled water, and according to solid-liquid mass ratio 1:5, redisperse in distilled water, prepare chitosan polydopamine suspension; Press chitosan polydopamine suspension and spicy Root peroxidase mass ratio i...

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Abstract

The invention relates to a preparation method of a composite polyphosphoester polydopamine biosensor and belongs to the field of biosensor preparation. The preparation method aims at solving the problem that a large amount of solvents are needed in the biosensor preparation process, and prepared biosensors have poor biocompatibility and cannot be effectively adhered to cells. Horse radish peroxidase is compounded and fixed by using a chitosan biomaterial as a basic layer, and then polydopamine can rapidly form films on the surfaces of various materials under the alkaline condition, contains a large amount of hydrophilic hydroxyls and amino functional groups and improves the hydrophilia and chemical multi-functionality of the material surfaces. The composite polyphosphoester polydopamine biosensor prepared by means of the preparation method has better biocompatibility and does not produce obvious rejection reaction after being implanted. The biosensor is prepared by utilizing chitosan and the polydopamine and accordingly is green, friendly to environment and is free of pollution.

Description

technical field [0001] The invention relates to a method for preparing a polyphosphate composite polydopamine biosensor, belonging to the field of biosensor preparation. Background technique [0002] With the development of information technology and bioengineering technology, biosensors have developed extremely rapidly. Today's developed countries have listed biosensors as a key technology in the 21st century and attached great importance to them. Biosensors are not only widely used in the field of traditional medicine to promote the development of medicine, but also widely used in the fields of space life science, food industry, environmental monitoring and military affairs. There are as many as dozens of enzyme sensors developed internationally, such as glucose, cholesterol, lactic acid and amino acid sensors. The early glucose biosensors used bovine serum albumin and glutaraldehyde to immobilize glucose oxidase into a membrane, cover it on the oxygen permeable membrane ...

Claims

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Application Information

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IPC IPC(8): G01N27/327
CPCG01N27/327
Inventor 高玉梅高力群
Owner CHANGZHOU YAHUAN ENVIRONMENTAL PROTECTION TECH