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Biological control method for tobacco rot-not nematodes diseases and biocontrol agent thereof

A tobacco root-knot nematode and root-knot nematode technology, applied in nematicides, biocides, botanical equipment and methods, etc., can solve the problem of reducing tobacco quality and eating safety, difficulty in breeding disease-resistant varieties, and prevention and control Problems such as unsatisfactory effect

Inactive Publication Date: 2016-06-15
昆明保腾生化技术有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical pesticide control often causes environmental pollution and reduces tobacco quality and food safety; breeding of disease-resistant varieties is difficult and costly, and most of them can only resist a single physiological race of root-knot nematode disease. The risk of area outbreaks; agricultural control measures are labor-intensive and time-consuming, and the control effect is generally unsatisfactory

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0078] After the emergence of tobacco seedlings, spray 1 compound microbial agent No. 1, compound microbial agent No. 2 and compound microbial agent No. 3 at 0.2 kg or 0.6 kg or 1.0 kg per mu, respectively, every 7 days or 10 days 200 times of liquid or 600 times of liquid or 1000 times of liquid, a total of 3 times, untreated tobacco seedlings as a control, transplanted to the same piece of field where root-knot nematode disease was severe in previous years after becoming seedlings, and managed according to conventional methods. The incidence of root-knot nematode disease in the root system of tobacco plants was investigated at the end of tobacco leaves harvesting and roasting. The survey results showed that after the emergence of tobacco seedlings, the compound microbial agent No. 1, compound microbial agent No. 2 and compound microbial agent No. 3 were sprayed three times respectively. The relative control effect against tobacco root-knot nematode is between 47.64 and 58.72%...

Embodiment 2

[0080] When growing flue-cured tobacco seedlings, mix the composite microbial agent No. 1, the composite microbial agent No. 2 and the composite microbial agent No. 3 into the commercially available floating seedling substrate, and mix 0.5kg or 2.5kg or 5kg of the composite microbial agent per 100kg of the seedling substrate. The untreated tobacco seedlings were used as a control, and transplanted to the same field where the root-knot nematode disease was severe in previous years, and managed according to conventional methods. The incidence of root-knot nematodes in tobacco plants was investigated at the end of tobacco leaf harvesting and roasting. The survey results showed that when the flue-cured tobacco seedlings were raised, the compound microbial agent No. 1, the compound microbial agent No. 2 and the compound microbial agent No. 3 were mixed into the seedling matrix respectively. The relative control effect against tobacco root-knot nematode is between 51.84% and 62.59%. ...

Embodiment 3

[0082] When growing flue-cured tobacco seedlings, mix the composite microbial agent No. 1, the composite microbial agent No. 2 and the composite microbial agent No. 3 into the commercially available floating seedling substrate, and mix 0.5kg or 2.5kg or 5kg of the composite microbial agent into each cubic seedling substrate. After the emergence of tobacco seedlings, spray 0.2kg or 0.6kg or 1.0kg per mu once every 7 days or 10 days, respectively. No. 3 200 times liquid or 600 times liquid or 1000 times liquid, a total of 2 times, untreated tobacco seedlings as a control, transplanted to the same piece of field where root-knot nematode disease was seriously infested in previous years, and managed according to conventional methods . The incidence of root-knot nematode disease in the root system of tobacco plants was investigated at the end of tobacco leaf harvesting and roasting. The survey results showed that: when the flue-cured tobacco was grown, the compound microbial agent N...

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PUM

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Abstract

The invention relates to a biological control method for tobacco rot-not nematodes diseases and a biocontrol agent thereof, pertaining to the field of prevention and control over tobacco diseases. The biocontrol agent method comprises following steps: colonization of antagonistic bacteria for root knot nematodes during tobacco seedling; colonization of antagonistic bacteria for root knot nematodes during transplantation of tobacco seedling land; and colonization of antagonistic bacteria for root knot nematodes during growth of tobaccos. The biocontrol agent is a compound microorganism bacterium agent and an anti-biont organic fertilizer for antagonistic bacteria for tobacco root knot nematodes. The compound microorganism bacterium agent is formed by compounding of two or multiple kinds of five bactericides including bacillus megatherium Ap25, azotobacter chroococcum YKT41, Vietnam burkholderia cenocepacia P418, green trichoderma LT-10, green trichoderma L-11 and trichoderma harzianum T11-W based on the certain proportion. The bio-organic fertilizer is obtained by preparation of 5-25% of the compound microorganism bacterium agent, 10-30% of protein residues, 5-30% of lignite, 10-25% of biochemical fulvic acid, 10-35% of biological charcoal and additional 0.5% -2.5% of calcium carbonate according to the mass ratio of the above mixture. The biological control method for tobacco rot-not nematodes diseases and the biocontrol agent thereof have following beneficial effects: the biocontrol agent helps effectively prevent and control tobacco rot-not nematodes diseases and is eco-friendly and safe; the method is easily operated and has high effect of preventing tobacco rot-not nematodes diseases.

Description

Technical field: [0001] The invention belongs to the field of tobacco disease prevention and control, and in particular relates to a method for biological control of tobacco root-knot nematode disease and a biological control preparation thereof. technical background: [0002] Tobacco root-knot nematode disease is caused by root-knot nematodes that invade the roots of tobacco plants. This nematode belongs to the phylum Nematodes, the class Nematodes, the order of the order, the order of the order, the order of the order, the family of the superfamily, the family of Heterodermis, and the genus of root-knot nematodes. In my country, root-knot nematode (Meloidogynein congnita Chitwood) is the main damage. [0003] Tobacco root-knot nematode disease can occur in both the seedling stage and the field stage, and mostly occurs in the field adult plant stage. Firstly, nodules of different sizes are formed on the roots of the infected tobacco plants, and the primary nodules on the ...

Claims

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Application Information

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IPC IPC(8): A01G13/00A01N63/04A01P5/00C05G3/02C05G3/04C05G3/60C05G3/80
CPCA01G13/00A01N63/30C05D3/02C05G3/60C05G3/80C05F5/00C05F11/02
Inventor 张廷金闫春丽尹忠仁李文荣王建光陈穗云扈进冬李纪顺杨合同李秀军
Owner 昆明保腾生化技术有限公司
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