A compound microbial agent for efficient degradation of food waste and its preparation method and application
A technology for compounding microbial inoculants and kitchen waste is applied in recycling technology, solid waste removal, furniture waste recycling and other directions, which can solve the problems of secondary pollution of life, hazards, and inability to meet the calorific value requirements of waste incineration power generation, etc. To achieve the effect of fast degradation and remarkable effect
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Embodiment 1
[0023] A preparation method for efficiently degrading a composite microbial bacterial agent from food waste, comprising the following steps:
[0024] (1) Cultivate bacterial strains and prepare composite bacterial cells.
[0025] ① Firstly, add the lyophilized powder of Issaccharomyces orientalis to 5ml of activated medium for shaking-table culture, and gradually enlarge it to 100L fermenter functional medium for cultivation. Cultivate in a fermenter at 32-34°C for 12-24 hours, then collect the bacteria by centrifugation, and freeze-dry to prepare dormant microorganism dry powder. The composition of the activation medium and the fermentation medium are: glucose 50-150g, yeast powder 3g, beef extract 6g, urea 0.3g, dipotassium hydrogen phosphate 0.1g, distilled water to 1L, pH value 5.5, extinguish at 115°C bacteria for 15 minutes.
[0026] ② First, add the freeze-dried powder of Bacillus subtilis to 5ml of activated medium for shaking-table culture, and gradually enlarge it ...
Embodiment 2
[0042] A preparation method for efficiently degrading a composite microbial bacterial agent from food waste, comprising the following steps:
[0043] (1) Cultivate bacterial strain, prepare composite thalline, this step is identical with embodiment 1;
[0044] (2) mixing the Issaccharomyces orientalis, Bacillus subtilis, Saccharomyces aberrantensis, Trichoderma niger and actinomycetes obtained by centrifugation according to the ratio of 10:18:8:15:18 to obtain a composite thalline;
[0045] (3) mixing soybean meal, bran, rice husk powder and shavings in a weight ratio of 2:4:6:8 to make a carrier;
[0046] (4) Mix the composite bacterium obtained in step (2) with the carrier obtained in step (3) to prepare a composite microbial inoculum, and the composite microbial inoculum accounts for 9% by mass of the composite microbial inoculum.
[0047]The degradation rate test of a kind of food waste efficient degradation composite microbial bacterial agent in the embodiment 2 of the p...
Embodiment 3
[0056] A preparation method for efficiently degrading a composite microbial bacterial agent from food waste, comprising the following steps:
[0057] (1) Cultivate bacterial strain, prepare composite thalline, this step is identical with embodiment 1;
[0058] (2) mixing the Issaccharomyces orientalis, Bacillus subtilis, Saccharomyces anomaly, Trichoderma niger and actinomycetes obtained by centrifugation according to the ratio of 15:25:10:20:20 to obtain a composite thalline;
[0059] (3) mixing soybean meal, bran, rice husk powder and shavings in a weight ratio of 3:6:7:9 to make a carrier;
[0060] (4) Mix the composite thallus obtained in step (2) with the carrier obtained in step (3) to prepare a composite microbial inoculum, and the composite microbial inoculum accounts for 12% by mass of the composite microbial inoculum.
[0061] The degradation rate test of a kind of food waste efficient degradation composite microbial bacterial agent in the embodiment of the present ...
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