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Self-assembly polypeptide d-RADA16-RGD and preparation method and application thereof

A d-rada16-rgd, self-assembly technology, applied in the field of biomaterials, can solve the problems of less research on self-assembled short peptides, achieve good tissue compatibility and biological activity, improve osteogenic ability, and be easy to operate

Inactive Publication Date: 2016-06-15
THE FIRST AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the present invention aims to solve the problem that most of the existing self-assembled short peptides are left-handed, and there are few researches on right-handed self-assembled short peptides, and provides a self-assembled polypeptide d-RADA16-RGD and its preparation method and application

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  • Self-assembly polypeptide d-RADA16-RGD and preparation method and application thereof
  • Self-assembly polypeptide d-RADA16-RGD and preparation method and application thereof
  • Self-assembly polypeptide d-RADA16-RGD and preparation method and application thereof

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Embodiment

[0045] 1. Synthesis and purification of self-assembling polypeptide d-RADA16-RGD

[0046] The self-assembling polypeptide d-RADA16-RGD is composed of right-handed amino acids, and the polypeptide sequence is Ac-(Arg-Ala-Asp-Ala-Arg-Ala-Asp-Ala) 2 -Gly-Gly-Arg-Gly-Asp-Ser-CONH. Shanghai Botai Biotechnology Co., Ltd. uses peptide solid-phase synthesis technology (SPPS) to synthesize peptide sequences, uses high-performance liquid chromatography (HPLC) to analyze and purify the synthesized peptides, and confirms the molecular weight by mass spectrometry. The purity of the polypeptide d-RADA16-RGD was 98.58%.

[0047] 1.1.1 Reagents

[0048] Fluorenylmethoxycarbonyl (FMOC)-amino acid is a product of SIAM Company in the United States; PyBOP, rinkamind resin, hexahydropyridine, and lutidine are products of Merck Company; Triketone Soak and Molecular sieve dehydration, and determination of no free amino groups); trifluoroacetic acid (TFA) is a product of GEELBELGIUM; methanol is...

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Abstract

The invention discloses a self-assembly polypeptide d-RADA16-RGD and a preparation method and application thereof. A polypeptide sequence is Ac-(Arg-Ala-Asp-Ala-Arg-Ala-Asp-Ala)2-Gly-Gly-Arg-Gly-Asp-Ser-CONH2. The self-assembly polypeptide d-RADA16-RGD is modified by a functional motif (FM) RGD and is obtained through the synthesis of amino acids, and a formed three dimensional stent has good histocompatibility and biological activity. The self-assembly polypeptide can serve as a carrier for slowly releasing growth factors and medicines, and the functional motif, such as the RGD, is also applied to short peptide modification, and the bone inductivity and osteogenic capability can be significantly improved. The preparation method is simple, convenient to operate and good in bone repair effect, has an important promoting role on bone repair and can be widely applied to clinical bone defect diseases.

Description

technical field [0001] The invention belongs to the technical field of biological materials, and in particular relates to a self-assembled polypeptide d-RADA16-RGD and its preparation method and application. Background technique [0002] Bone defect refers to the lack of bone due to trauma, infection, tumor resection, or congenital disease, which destroys the continuity and integrity of the bone and forms a large gap. With the increase of construction, traffic accidents and population aging, the number of patients with bone defects is increasing, which has an important impact on the quality of life of patients and the investment in social health services. Large bone defects cannot heal on their own, and their repair requires natural or artificial material scaffolds to provide structural support and osteoinduction. How to select or prepare generalizable natural or artificial bone repair materials with both good bioactivity and appropriate biomechanical properties is the prer...

Claims

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Application Information

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IPC IPC(8): C07K19/00C07K1/06C07K1/04A61L27/22A61L27/54
CPCA61L27/22A61L27/54A61L2300/252A61L2300/412A61L2430/02C07K7/08C07K2319/00Y02P20/582
Inventor 蒋电明何彬陈硕周敖赵维康
Owner THE FIRST AFFILIATED HOSPITAL OF CHONGQING MEDICAL UNIVERSITY
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