Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Novel encoding microsphere and preparation method thereof

A coded microsphere, a new type of technology, applied in the field of composite materials, can solve the problems that the signal cannot meet the actual needs, the strength of the fluorescent signal changes, and the capacity of the code is reduced, so as to improve the stability and preparation efficiency, enhance the coating effect, The effect of increasing the encoding capacity

Inactive Publication Date: 2016-06-22
FUZHOU UNIV
View PDF5 Cites 6 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, when several Raman molecules are used together as a code, the overlapping of characteristic peaks will inevitably occur, which will greatly reduce the capacity of the code.
In addition, for fluorescently encoded microspheres, there is also the problem of overlapping emission peaks
For example, fluorescence energy transfer often leads to excitation, emission peak overlap and fluorescence signal intensity changes, so the fluorescent dyes and quantum dots that can be used for actual coding are much less than the theoretical ones, so they can be used to mark the coding signal so far. Still far from meeting actual needs

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel encoding microsphere and preparation method thereof
  • Novel encoding microsphere and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Coded microspheres with a Raman and a fluorescent coded signal

[0025] The specific steps of its preparation are as follows:

[0026] 1) Add 0.2g of melamine and 0.6g of formaldehyde to 100mL of deionized water, adjust the pH of the system to 4 with hydrochloric acid, add fluorescent probe molecules and then add 5mg of fluorescent probe molecules FITC, react for 2h and then stand still to remove the upper layer Emulsion, the obtained product was washed three times with water and ethanol, and the product was separated by centrifugation, and then dried in vacuum at 50°C to obtain monodisperse MF fluorescent microspheres with uniform size. The SEM scan and fluorescence micrographs of the microspheres are shown respectively figure 1 (A) and figure 1 (B);

[0027] 2) Add the MF fluorescent microspheres obtained in step 1) to 20mL containing AgNO 3 Add 2mg of sodium borohydride to 2mg of ethanol solution, then sonicate the mixture for 2min, then place it on a shaker, ...

Embodiment 2

[0032] Example 2: Coded microspheres with one Raman and two fluorescent coding signals

[0033] 1) Add 0.625g of melamine and 0.9g of formaldehyde to 100mL of deionized water, adjust the pH of the system to 4 with hydrochloric acid, add 30mg of fluorescent probe molecules FITC and PHB, respectively, react for 2h, then let it stand and remove the upper emulsion. The product was washed three times with water and ethanol, and the product was separated by centrifugation, and then dried under vacuum at 50°C to obtain monodisperse MF fluorescent microspheres of uniform size;

[0034] 2) Add the MF fluorescent microspheres obtained in step 1) to 20mL containing AgNO 3 To 8mg ethanol solution, add 8mg sodium borohydride, and then ultrasonically treat the mixture for 2min, then place it on a shaker, shake at 50℃ for 60min, centrifuge, wash twice with ethanol, and dry at 50℃ for 2h to obtain MF / Ag-NPs composite microspheres;

[0035] 3) Disperse 10 mg of the MF / Ag-NPs composite microspheres...

Embodiment 3

[0038] Example 3: Dual-encoded microspheres with two kinds of Raman and two kinds of fluorescent coding signals

[0039] The specific steps of its preparation are as follows:

[0040] 1) Add 3g of melamine and 2g of formaldehyde to 100mL of deionized water, adjust the pH of the system to 4 with hydrochloric acid, add 40mg of fluorescent probe molecules FITC and PHB, respectively, react for 2h and let stand to remove the upper emulsion, the resulting product is water Wash three times with ethanol, centrifuge to separate the product, and then vacuum dry at 50°C to obtain MF fluorescent microspheres of uniform size and monodisperse;

[0041] 2) Add the MF fluorescent microspheres obtained in step 1) to 20mL containing AgNO 3 Add 15mg of sodium borohydride to 10mg of ethanol solution, and then ultrasonically treat the mixture for 2min, then place it in a shaker, shake for 60min at 50℃, wash with ethanol 3 times after centrifugation, and vacuum dry at 50℃ for 2h to obtain MF / Ag-NPs co...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a novel encoding microsphere and a preparation method thereof and belongs to the technical field of composites.The preparation method includes: using melamine resin as a base material and using Raman spectrum and fluorescent spectrum as encoding elements, wrapping fluorescent probe molecules in situ into a melamine resin microsphere by means of sol-gel method to obtain an MF fluorescent microsphere, coating the surface of the microsphere with an Ag nanoparticle layer to form an MF / Ag-NPs composite microsphere so as to impart good biocompatibility, and preparing the novel encoding microsphere responsive to both surface enhanced Raman and fluorescence.The obtained encoding microsphere has extended encoding capacity, can greatly reduce encoding signal interference due to spectral overlap so as to enhance the stability of encoding signals, and has a good application prospect in terms of biological detection.

Description

technical field [0001] The invention belongs to the technical field of composite materials, and in particular relates to a novel coded microsphere and a preparation method thereof. Background technique [0002] The rapid and highly sensitive detection of biomolecules such as antibodies, antigens, and DNA is of great significance to the diagnosis and treatment of diseases. However, a large number of screening and analysis of target molecules are usually required during the detection process, therefore, suitable methods are required to cope with the large number of biomarker analysis. The coded microsphere detection technology is to immobilize the target molecule on the surface of the microsphere with a specific code, and then identify the target molecule carried on the surface according to the coded signal of the coded microsphere. corresponding analytes. At present, the encoding methods of microspheres mainly include position graphic encoding and spectral encoding. Variou...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): B01J13/02C09K11/06G01N21/64G01N21/65
CPCB01J13/02C09K11/06C09K2211/1088C09K2211/14G01N21/64G01N21/65
Inventor 游力军汪长春王傲张其清
Owner FUZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com