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Method for promoting Hirsutella sinensis to produce conidiophores by mushroom dreg culture

A technology of Chinese hairy spores and conidia, applied in the field of microorganisms, to achieve the effect of increasing the number, strong vitality, and good stability

Active Publication Date: 2016-06-22
YICHANG SHANCHENGSHUIDU CORDYCEPS
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

For example, the document "Research on the production of conidia by different factors on the fungus Cordyceps sinensis" (Li Yuling, "Edible Fungus" 2002 No. 5), which discusses the production of conidia by Chinese hairy sporophytes in different media, different light and different temperatures. The research of spore; Another example is the literature "The impact of different trace elements and growth factors on the yield of Cordyceps sinensis conidia" (Li Lingling, Luo Hechun, Hu Min, "Journal of Chongqing Industry and Trade Vocational and Technical College" 2014 No. 2 period), which was passed in Adding trace elements and amino acid growth factors to the basic culture and formula, studied the effect of different trace elements and growth factors on the yield of Mortierella chinensis, but the number of conidia obtained by using the method in the above literature is not higher than 10 5 Magnitude

Method used

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  • Method for promoting Hirsutella sinensis to produce conidiophores by mushroom dreg culture
  • Method for promoting Hirsutella sinensis to produce conidiophores by mushroom dreg culture

Examples

Experimental program
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Effect test

Embodiment 1

[0028] Medium formula (mass percentage): silkworm chrysalis powder 0.5%, peptone 1%, yeast powder 2%, glucose 2%, anhydrous magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.1%, zinc sulfate heptahydrate 0.0013%, aspartate Acid 0.1%, vitamin C 0.1%, pure milk 8%.

[0029] Collect wild fresh Cordyceps sinensis ascospores, pick out monoascospores through a stereoscope and culture, after purification and culture, obtain the test tube slope of the Chinese Trichosporium monoasospore colony; And pick a few pieces of 0.5cm 2 Bacteria of about size are inoculated into a medium that can obtain a large amount of mycelium, and the clumps are evenly broken with a sterile homogenizer, and cultured in a constant temperature shaker at 120 rpm and 20 °C for 10 days, which is recorded as a first-class shaker. , expand the first-level shake flask, cultivate it under the same culture conditions for 5 days, record it as the second-level shaker, and so on. That is, bacterial residue; div...

Embodiment 2

[0032] Medium formula (mass percentage): silkworm chrysalis powder 0.5%, peptone 1.5%, yeast powder 1%, glucose 3%, anhydrous magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.1%, zinc sulfate heptahydrate 0.0013%, aspartate Acid 0.1%, vitamin C 0.1%, pure milk 8%.

[0033]Collect wild fresh Cordyceps sinensis ascospores, pick out monoascospores through a stereoscope and culture, after purification and culture, obtain the test tube slope of the Chinese Trichosporium monoasospore colony; And pick a few pieces of 0.5cm 2 Bacteria of about size are inoculated into a medium that can obtain a large amount of mycelium, and the clumps are evenly broken with a sterile homogenizer, and cultured in a constant temperature shaker at 120 rpm and 20 °C for 10 days, which is recorded as a first-class shaker. , expand the first-level shake flask, cultivate it under the same culture conditions for 5 days, record it as the second-level shaker, and so on. That is, bacterial residue; di...

Embodiment 3

[0036] Medium formula (mass percentage): silkworm chrysalis powder 0.5%, peptone 1%, yeast powder 2%, glucose 2%, anhydrous magnesium sulfate 0.05%, potassium dihydrogen phosphate 0.1%, zinc sulfate heptahydrate 0.0013%, aspartate Acid 0.1%, vitamin C 0.1%, pure milk 8%.

[0037] Collect wild fresh Cordyceps sinensis ascospores, pick out monoascospores through a stereoscope and culture, after purification and culture, obtain the test tube slope of the Chinese Trichosporium monoasospore colony; And pick a few pieces of 0.5cm 2 Bacteria of about size are inoculated into a medium that can obtain a large amount of mycelium, and the clumps are evenly broken with a sterile homogenizer, and cultured in a constant temperature shaker at 120 rpm and 20 °C for 10 days, which is recorded as a first-class shaker. , expand the first-level shake flask, cultivate it under the same culture conditions for 5 days, record it as the second-level shaker, and so on. That is, bacterial residue; div...

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Abstract

The invention discloses a method for promoting Hirsutella sinensis to produce conidiophores by mushroom dreg culture. The method comprises the four steps of strain selection, mushroom dreg acquisition, mushroom dreg growth and conidiophore induction. According to the method, high-stability high-activity aweto single ascospores capable of fermenting a large amount of mycelia are used as a strain; and the hyphae are subjected to multistage propagation and subjected to photoperiod stimulated culture by using different light sources in the conidiophore induction stage, thereby obtaining the high-yield Hirsutella sinensis conidiophores (1011-1012) within a short time. Thus, the method has important meanings for industrialized development of aweto artificial culture.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a method for promoting the production of conidia from Trichospora sinensis by culturing the slag. technical background [0002] Cordyceps sinensis is a complex of the seed (fruiting body) and the larvae that grow on the larvae of bat moth insects infected and parasitized by Cordyceps sinensis. It is an edible fungus and is a traditional and precious nourishing Chinese medicinal material It has various effects such as regulating immune system function, anti-tumor and anti-fatigue. [0003] Wild Cordyceps sinensis only grows in alpine meadows, plateau meadows and other alpine vegetation on the Qinghai-Tibet Plateau at an altitude of 3,000-5,000 meters. Due to over-excavation in recent years, Cordyceps resources are on the verge of extinction. On the other hand, with the improvement of people's living standards, the demand for Cordyceps sinensis is getting higher and higher, and artif...

Claims

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Application Information

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IPC IPC(8): C12N3/00C12R1/645
CPCC12N3/00
Inventor 李文佳张宗耀李全平吕延华何俊
Owner YICHANG SHANCHENGSHUIDU CORDYCEPS
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