Super-resolution imaging device and method

Abstract

The invention discloses a super-resolution imaging device and method. The device comprises a blue-light LED light source, a convex lens L1, a filter O1, a spectroscope, an encoder, a filter O2, a convex lens L2, a CCD (charge coupled device) camera and a computer, wherein blue light emitted by the blue-light LED light source passes through the convex lens L1, the filter O1, the spectroscope and the encoder sequentially and is irradiated on a tested sample, and emitted light produced by the tested sample passes through the encoder, the spectroscope, the filter O2 and the convex lens L2 sequentially and is sent into the computer by the CCD camera. The encoder adopts a random nanoparticle encoder. The microscopic device and the method are characterized in that a principle of a traditional optical microscope is adopted, decoding is realized completely through the computer, and the structure is simple; the LED light source is adopted to excite samples, laser irradiation is not needed, and the extent of damage to living cells is low.

Description

technical field [0001] The invention relates to a microscopic imaging device and method, in particular to a device and method for microscopic imaging of living cells by breaking through the diffraction limit of an optical microscope and achieving nanoscale resolution. Background technique [0002] The development of modern biology has put forward higher and higher resolution requirements for microstructure research, hoping to reveal the physical essence of life processes from the molecular level. However, limited by the optical diffraction limit, the lateral resolution of ordinary optical microscopes can only reach 200nm and the vertical resolution can reach 500nm, which makes it impossible to study the subcellular structure and the interaction of its internal substances. Although electron microscopy and atomic force microscopy can achieve nanometer-level resolution, their shortcomings in the observation of non-viable isolated cell samples limit their wide application in the...

AI Technical Summary

Problems solved by technology

Commonly used super-resolution techniques mainly include the following methods: 1) 4Pi microscopy: this technology can achieve nanometer resolution and in vivo measurement, but requires the use of interference methods and the complex structure of dual objective lenses; 2) stimulated emission loss microscopy : This technology can break through the far-field optical microscopy of optical diffraction to obtain super-resolution images, but this technology requires the use of supercontinuum lasers and long-term laser irradiation will destroy living cells; 3) Light-activated localization microscopy technology: this technology can Observe molecular activities at the nanometer level, but need to use specific wavelengths of light, repeated excitation and bleaching of fluorescent molecules, complex structures, and extremely low measurement efficiency

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